Resin acids and Rosin acids, barium salts

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Resin acids and Rosin acids, barium salts

EC Number:

269-142-0

EC Name:

Resin acids and Rosin acids, barium salts

Cas Number:

68188-14-7

Molecular formula:

N/A

IUPAC Name:

Resin acids and Rosin acids, barium salts

Test material form:

solid

Details on test material:

Specific details on test material used for the study
SOURCE OF TEST MATERIAL
- Batch number of test material: EXP NR.3757-01
- Expiration date of the lot/batch: 31 Dec 2029
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: refrigerator
- Stability under storage conditions: The stability of the test substance under storage conditions is
guaranteed until 31 Dec 2029 as indicated by the sponsor, and the sponsor holds this responsibility.
- Stability under test conditions: no data

Specific details on test material used for the study:

Test substance No.: 19/0421-1
Batch identification: EXP NR.3757-01

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal waste water treatment plant Mannheim, Germany
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

20 mg/L

Based on:

TOC

Initial test substance concentration 2

Initial conc.:

34 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 2 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no

SAMPLING
- Sampling frequency: every other day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control

Reference substance

Reference substance:

aniline

Remarks:

20 mg/L TOC

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

73

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Degree of biodegradation of the reference substance
after 14 days:
83% CO2/ThCO2
Degree of biodegradation in the inhibition control
after 14 days:
69% CO2/ThCO2

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Resin acids and Rosin acids, barium salts - EXP NR.3757-01
was readily biodegradable under the conditions of this test
over the 28-day exposure to activated sludge according to
OECD guideline 301B.
The required pass level for ready biodegradability i.e., at
least 60% of the theoretical CO2 production within the 28-day
period of the test was reached.
Because the test substance is a UVCB, the criterion of the
ten-day window for the assessment of the ready
biodegradability was not applied.