Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 236-695-4 | CAS number: 13465-41-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20. October - 22. October 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Instead of standard M4 medium, water treated with salts (specification given below) was used. This change is in line with the OECD guideline for testing metals. As no detrimental effects were observed, the deviations can be considered uncritical.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on test solutions:
- For testing the following specification was used (instead of standard M4 medium):
293.8 mg/L CaCl2*2H2O, 123.3 mg/L MgSO4*7H2O, 64.8 mg/L NaHCO3 and 5.8 mg/L KCl
Deviations from the nominal weighted loads were less than 5 % during preparation. After preparation, the dilution water was aerated and the pH was measured. The pH was 7.6. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Strauss Berlin
- Justification for species other than prescribed by test guideline: -
- Age at study initiation (mean and range, SD): between 0 and 24 hours
- Method of breeding: parthenogenesis
- Source: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD): -
- Feeding during test
- Food type: -
- Amount: -
- Frequency: -
ACCLIMATION
- Acclimation period: no
- Acclimation conditions (same as test or not): -
- Type and amount of food: -
- Feeding frequency: -
- Health during acclimation (any mortality observed): no
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.3 – 20.7 °C
- pH:
- 7.8 - 7.9
- Dissolved oxygen:
- 8.3 - 8.9
- Salinity:
- -
- Nominal and measured concentrations:
- nominal: 0.46 / 1 / 2.2 / 4.6 / 10 mg/L (related to test item)
geometric measured mean: 0.016 / 0.027 / 0.071 / 0.144 / 0.276 mg/L (related to permanganic acid) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, ca. 30 mL headspace, 20 ± 5 mL fill volume
- Volume of solution:
- Aeration: -
- Type of flow-through (e.g. peristaltic or proportional diluter): -
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): -
- Biomass loading rate: -
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demin. water; additional information under 'details on test solutions'
- Total organic carbon: -
- Particulate matter: -
- Metals: -
- Pesticides: -
- Chlorine: -
- Alkalinity: -
- Ca/mg ratio: approx 3:1
- Conductivity: -
- Salinity: -
- Culture medium different from test medium: yes (standard M4 medium for culture)
- Intervals of water quality measurement: -
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours, using LED’s
- Light intensity: not given
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation (24 / 48 h)
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY (non-GLP range finder was performed)
- Test concentrations: not stated
- Results used to determine the conditions for the definitive study: not stated - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a cur-rent reference study to assure that the test conditions are reliable.
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.039 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.044 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.027 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.071 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: -
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: -
- Immobilisation of control: no
- Abnormal responses: -
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No undissolved particles were visible in all test solutions throughout the test
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- not stated
- Reported statistics and error estimates:
- Statistics were calculated with ToxRat Professional (V.3.3.0). Confidence interval was not calculated.
- Validity criteria fulfilled:
- yes
- Remarks:
- no immobilisation in controls was observed; the lowest concentration of dissolved oxygen at the end of the test was 8.3 mg/L (limit 3 mg/L).
- Conclusions:
- The dose-response relationship was extremely steep. An effect concentration EC50 (48 h) of 0.044 mg/L was determined in this study.
- Executive summary:
The study was performed using 5 concentrations ranging from 0.46 to 10 mg/L nominal concentration. For each test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
The dose-response relationship was extremely steep. The three highest concentrations showed toxicity of 100 % immobilisation. None of the animals was immobilised in the blank control and the lower concentrations.
Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of Manganese (Mn) as component of the test item in the test solutions was determined using ICP-OES-determination. The concentrations determined at the start of the test lay between 99 % and 112 % of the nominal concentrations and between 55 % and 99 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the geometric mean of the measured concentrations (see OECD 202 (§ 23)).
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
The result of the test is considered valid.
Reference
Nominal Concentration in mg/L |
Geometric Mean Concentration in mg/L
|
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
||||||||
Blank control |
-- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.46 |
0.016 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0.027 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2.2 |
0.071 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
4.6 |
0.144 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
10 |
0.276 |
5 |
5 |
5 |
5 |
100 |
5 |
5 |
5 |
5 |
100 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.044 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.