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EC number: 701-365-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 16. to 23 October 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0, 1, 10, 100, 500 mg/L
- Vehicle:
- yes
- Details on test solutions:
- TS was diluted in water by ultra sonification and distributed evenly in the tank by means of a glass stirrer.
- Test organisms (species):
- Leuciscus idus melanotus
- Details on test organisms:
- TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus melanotus
- Source: Paul Eggers, Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 5.5 to 6.5 cm; mean: 6 cm; SD: 0.37
- Weight at study initiation (mean and range, SD): 1.7 to 2.5 g; mean: 2.1 g; SD: 0.27; n=10
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions:
- Dilution water (ISO/DIS 7346/1)
- 20 °C
- pH 8.32 to 8.4
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice/day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- 96 hours
- Hardness:
- total: 9.5 °d
carbonate: 6.4 °d - Test temperature:
- 19 to 21 °C
- pH:
- 7.9 to 8.4
- Dissolved oxygen:
- 8.2 to 8.9 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- nominal: 0, 1, 10, 100, 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Glass; 40x25x30 cm³; 20 liter water
- Aeration: 100 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): at least one
- No. of vessels per control (replicates): at least one
- No. of vessels per vehicle control (replicates): NA
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized tap water (Christ-Ministil Ion-Exchanger cartridge) conductance value < 5 µS/cm enriched with:
192 mg/L NaHCO3
120 mg/L CaSO4 x 2H2O
120 mg/L MgSO4
8 mg/L KCl
- Conductivity: < 5 µS/cm
- Intervals of water quality measurement: directly prior TS, after 2, 24, 48, 72, 96 hours
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h
- Light intensity: ca. 700 Lux
EFFECT PARAMETERS MEASURED
Mortality, behavior, macroscopic abnormities - Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 100 - 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: 500 mg/L deaths between 23 and 95 hours; swimming at water surface; supine position; no effects at 1 to 100 mg/L
- Necropsy:dead animals: blue discoloration of fins and mouth; brown-green discoloration of bile
100 mg/L: test substance deposition in GI-tract; white crystals in gall-bladder
no effects at 1 to 10 mg/L
- Mortality: 48 h: 1/10 at 500 mg/L; 96 h: 10/10 at 500 mg/L
- Mortality of control: no effects
- Other adverse effects control: no effects
- Abnormal responses: no effects
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
- Other: due to the staining nature of the TS, the water was dark-blue discolored - Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- NA
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC0 and LC100 after 48 and 96 hours is between 100 and 500 mg/L. Hence, its concluded that 96 h LC50 of the test substance to fish is >100 mg/l.
- Executive summary:
In an 96 h acute fish toxicity test, the test substance was assessed by following method comparable to today’s standard testing procedure published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17thJuly 1992. The fish were exposed to the test substance at 0, 1, 10, 100, 500 mg/L concentrations and observed till 96 h for mortality.
The LC0 and LC100 after 48 and 96 hours is between 100 and 500 mg/L. Hence, its concluded that 96 h LC50 of the test substance to fish is >100 mg/l.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18. to 25 April 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0, 1, 10, 100, 500 mg/L
- Vehicle:
- yes
- Details on test solutions:
- The test substance was diluted in water by ultra sonification and distributed evenly in the tank by means of a glass stirrer.
- Test organisms (species):
- Leuciscus idus melanotus
- Details on test organisms:
- TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus melanotus
- Source: Paul Eggers, Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 5.4 to 6.3 cm; mean: 5.9 cm; SD: 0.35
- Weight at study initiation (mean and range, SD): 1.4 to 2.6 g; mean: 1.8 g; SD: 0.37; n=10
- Type of food: Tetra Min, Tetra Werke, Melle
ACCLIMATION
- Acclimation period: at least 14 days at 20 °C
- Type of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice/day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- NA
- Hardness:
- total: 9.5 °d
carbonate: 6.4 °d (1.14 mmol/L) - Test temperature:
- 19 to 21 °C
- pH:
- 8.2
- Dissolved oxygen:
- above 7 mg/L
- Nominal and measured concentrations:
- nominal: 0, 1, 10, 100, 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Glass; 40x25x30 cm³; 20 liter water
- Aeration: 100 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): at least one
- No. of vessels per control (replicates): at least one
- No. of vessels per vehicle control (replicates): NA
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized tap water (Christ-Ministil Ion-Exchanger cartridge) conductance value < 5 µS/cm enriched with:
192 mg/L NaHCO3
120 mg/L CaSO4 x 2H2O
120 mg/L MgSO4
8 mg/L KCl
- pH: 8.0 to 8.4
- Oxygen: 7.6 to 8.3 mg/L
- Conductivity: <5 µS/cm
- Intervals of water quality measurement: directly prior TS, after 2, 24, 48, 72, 96 hours
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h
- Light intensity: ca. 700 Lux
EFFECT PARAMETERS MEASURED
Mortality, behavior, macroscopic abnormities - Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 100 - 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 100 - 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: 500 mg/L deaths at 46 hours; swimming at water surface; reduced gill movements; no effects at 1 to 100 mg/L
- Necropsy:500 mg/L: blue discoloration of fins and gills and the upper part of the vitreous body
no effects at 1 to 100 mg/L
- Mortality: 48 h: 10/10 at 500 mg/L; 96 h: 10/10 at 500 mg/L
- Mortality of control: no effects
- Other adverse effects control: no effects
- Abnormal responses: no effects
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
- Other: due to the staining nature of the TS, the water was dark-blue discolored - Validity criteria fulfilled:
- yes
- Conclusions:
- The LC0 and LC100 after 48 and 96 hours is between 100 and 500 mg/L. Hence, its concluded that 96 h LC50 of the test substance to fish is >100 mg/l.
- Executive summary:
In an 96 h acute fish toxicity test, the test substance was assessed by following method comparable to today’s standard testing procedure published by the OECD within Guideline for Testing of Chemicals 203: Fish, Acute Toxicity Test, adopted by the Council on 17th July 1992. The fish were exposed to the test substance at 0, 1, 10, 100, 500 mg/L concentrations and observed till 96 h for mortality.
The LC0 and LC100 after 48 and 96 hours is between 100 and 500 mg/L. Hence, its concluded that 96 h LC50 of the test substance to fish is >100 mg/l.
Referenceopen allclose all
Description of key information
The LC50 of the test substance to freshwater fish is >100 mg/l. Hence, the substance is not toxic to aquatic vertebrates.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
In a publication from Ministry of the Environment Government of Japan, 96 h acute toxicity test with test substance was assessed according to OECD Guideline 203 and in compliance with GLP. Fish, Oryzias latipes, were exposed to single concentration of the test substance at 100 mg/L and observed for 96h. No mortality was observed. The LC50 of the test item to fish after 96 hours is determined to be >100 mg/L.
Two other supporting studies which were performed following OECD Guideline 203 showed LC50 value of >500 mg/L.
Reactive Black 5 rapidly degrades to its oxy-form in water. It is therefore reasonable to test this main metabolite in aquatic toxicity tests. Data from a read-across substance generated by following a OECD 203 guideline in compliance to GLP is taken into consideration here to analyze the toxicity of the test substance. The LC50 of the read-across substance in freshwater fish after 96 hours was also noted to be above 500 mg/L
Taking data from above mentioned studies into account and also data from the read across substance, the LC50 of the test substance to freshwater fish is >100 mg/l. Hence, the substance is not toxic to aquatic vertebrates.
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