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EC number: 951-963-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30/04/2020-16/07/2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- Constituent
- Type:
- Constituent
- Test material form:
- other: wet sticky solid
- Specific details on test material used for the study:
- Designation in Test Facility: 19101604G
Date of Receipt: 16. Oct. 2019
Condition at Receipt Ambient temperature, in proper conditions
5.1.1 Specification
The following information concerning identity and composition of the test item was provid-ed by the sponsor.
Name zinc chlorohydroxy sulphate and copper sulphide, precipitation
Batch no. 20190601
CAS no. unknown
Composition 2.9 % Cu; 18.0 % Zn; 0.5 % Fe; 0.1 % Pb; 0.7 % Cd; 0.4 % As; 0.3 % Sb; 0.1 % Sn; 0.0 % Bi; 0.0 % Br; 2.7 % Cl; 1.1 % F
Storage room temperature (20 ± 5 °C)
Expiry date 31. Dec. 2025
Stability stable under storage conditions
Appearance blackish-green, wet sticky solid
Purity 100 % (UVCB)
Homogeneity homogeneous
Production date not stated
EC no. unknown
Molecular formula not stated
Molecular weight not stated
Vapour pressure not stated
Solubility in solvents H2O: not stated; EtOH: not stated; acetone: not stated; CH3CN: not stated; DMSO: not stated
Stability in solvents H2O: not stated; EtOH: not stated; acetone: not stated; CH3CN: not stated; DMSO: not stated
A certificate of analysis is provided by the sponsor and attached (in copy) in chapter 16. This analytical report is only for informative purpose. The composition might differ in re-gards of percentage values from batch to batch
5.1.2 Storage
The test item was stored in the test facility in a closed vessel at room temperature (20 ± 5 °C).
5.1.3 Preparation
Since the test item is a poorly soluble UVCB the water-accommodated fraction (WAF) was prepared for the test. This was done by mixing 10.2 mg/L with the corresponding amount of algal medium (demineralised water enriched with minerals but without algae) and shak-ing vigorously for 23.5 hours. After a settling time of 1 hour the resulting solution was fil-trated through 0.45 µm nylon filters.
The lower treatments (0.1 / 0.32 / 1 / 3.2 mg/L) were prepared by dilution of this WAF with nutrient medium. This procedure is in agreement with the OECD guidance document no. 23 for the testing of mixtures which are toxic at very low loading rates. In a non GLP pre-test at the loading rate 1 mg/L clear inhibition of algal growth was observed.
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Selection of the test system was made following the proposal of the guidelines.
5.3.1 Specification
Unicellular freshwater green alga.
Genus Desmodesmus
Species subspicatus
SAG Strain Number 86.81
Taxonomic position Chlorophyta - Chlorophyceae
5.3.2 Origin and Culture
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sci-encebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was pre-pared
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Details on test conditions:
- Date: 19. – 22. May 2020
Treatments tested: 0.1 / 0.32 / 1 / 3.2 / 10 mg/L nominal concentration
The concentrations to be tested are based on non GLP pre-tests
Vessels: glass flasks total volume 65 mL
Duration: 72 hours
Lighting: within the specified range (4440 – 8880 Lux)
Temperature: 20.7 – 22.3 °C
Replicates
Treatments: 3 vessels, filled with 45 ±1 mL test solution and algae
1 vessels, filled with 45 ±1 mL test solution without algae for analytical determination
Blank Control: 6 vessels, each completely filled with algal medium and algae
1 vessel completely filled with algal medium without algae for analytical determination - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.03 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.75 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- At the start and at the end of the test, the content of Zn as part of the test item in the test solutions was determined using ICP-analysis.
With the help of this determination method it could be proven that the test item was pre-sent in the test solutions. Since the test item is poorly soluble, it is not possible to calculate back from the Zn concentration to the test item concentration. Therefore, the nominal concentrations are used for statistical evaluation.
For statistical evaluation, the lowest concentration is not used because it is not necessary. The next two higher concentrations also show a stimulation of algae growth.
Calculation of results was performed with the help of validated software (Microsoft Ex-cel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.3.0. The details of calculation are stated in chapter 18.
The following values were determined.
Table 9 a Biological Results of the Test Item
Parameter Value 95 % confidence interval
NOEL (Growth Rate) 72 h 1.0 mg/L --
NOEL (Yield) 72 h 1.0 mg/L --
LOEL (Growth Rate) 72 h 3.2 mg/L --
LOEL (Yield) 72 h 3.2 mg/L --
72h ErL10 3.37 mg/L 3.10 – 3.63 mg/L
72h EyL10 1.94 mg/L 1.81 – 2.05 mg/L
72h ErL50 8.03 mg/L 7.77 – 8.29 mg/L
72h EyL50 3.75 mg/L 3.66 – 3.85 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 0.1 to 10 mg/L (nominal). Incubation time (test systemDesmodesmus subspicatus)was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield[1]were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 10 mg/L (nominal) for yield and growth rate
At the start and at the end of the test, the content of Zn as part of the test item in the test solutions was determined using ICP-analysis.
With the help of this determination method it could be proven that the test item was present in the test solutions. Since the test item is a poorly soluble UVCB, it is not possible to calculate back from the Zn concentration to the test item concentration. Therefore, the nominal concentrations are used for statistical evaluation.
The 72h-EC50values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.65 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test itemzinc chlorohydroxy sulphate and copper sulphide, precipitationwere determined:
Table 3‑a Results of the test item
Endpoint
NOEL
LOEL
EL10
EL50
Growth Rate
1 mg/L
3.2 mg/L
3.37 mg/L
8.03 mg/L
Yield
1 mg/L
3.2 mg/L
1.94 mg/L
3.75 mg/L
[1]Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 7.2 Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. Algal biomass is defined as the dry weight per volume, e.g. mg algae/L test solution. However, dry weight is difficult to measure and therefore surrogate parameters are used. Of these surrogates, cell counts are most often used.
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