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EC number: 949-986-2 | CAS number: 49720-39-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-09-30 to 1992-05-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guidline study whit acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Arginine
- EC Number:
- 200-811-1
- EC Name:
- Arginine
- Cas Number:
- 74-79-3
- Molecular formula:
- C6H14N4O2
- IUPAC Name:
- arginine
- Test material form:
- not specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was weighed out and each portion was dissolved in 1l dilution water.
- Eluate: the dilution water used was DSWL, prepared from groundwater.
- Controls: DSWL (negative control).
- Evidence of undissolved material (e.g. precipitate, surface film, etc) visually.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Common name: zebrafish
- Source: M.B. Ruysbroek B.V., Noordvliet 159, NL-Maassluis
- Length at initiation (length definition, mean, range and SD): 2.2 +/- 0.1 cm (SD)
- Weight at study initiation (mean and range, SD): 0.09 +/- 0.02 g (SD)
- Feeding during test: no
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Hardness, expressed as CaCO3: 204 mg/l
- Test temperature:
- 24.2 to 24.6 °C
- pH:
- Control medium: Begining of test: 7.8 to 8.0 End of test 7.6 to 7.9
1 g/l test substance: Begining of test: 9.3 to 9.5 End of test 8.6 to 8.8
1.8 g/l test substance: Begining of test: 9.5 to 9.7 End of test 8.8 to 9.0
3.2 g/l test substance: Begining of test: 9.7 to 9.9 End of test 9.1 to 9.2
5.6 g/l test substance: Begining of test: 9.9. End of test 9.5
10 g/l test substance: Begining of test: 10.1 End of test 9.7 - Dissolved oxygen:
- Control medium: Begining of test: 7.8 to 8.4 mg/l. End of test 7.2 to 8.3 mg/l.
1 g/l test substance: Begining of test: 7.8 to 8.4 mg/l. End of test 7.2 to 9.1 mg/l.
1.8 g/l test substance: Begining of test: 7.9 to 8.3 mg/l. End of test 7.3 to 8.2 mg/l.
3.2 g/l test substance: Begining of test: 7.9 to 8.3 mg/l. End of test 7.2 to 8.2 mg/l.
5.6 g/l test substance: Begining of test: 8.1 mg/l. End of test 8.3 mg/l.
10 g/l test substance: Begining of test: 8.3 mg/l. End of test 8.4 mg/l. - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations of testsubstance: 1.0, 1.8, 3.2, 5.6, 10 g/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: all-glass beakers
- Type: open
- Material, size, headspace, fill volume: glass, 2000 ml
- Aeration: slightly aerated
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Groundwater from Linschoten (the Netherlands). The medium is prepared by adding several salts.
- Total organic carbon: 1.5 mg/l
- Metals: << 1 mg/l
- Chlorine: 2.71 mml/l
- Na+: 1.19 mmol/l
- K+: 0.22 mmol/l
- Ca 2+: 1.30 mmol/l
- Mg2+. 0.74 mmol/l
- SO42-: 0.69 mmol/l
- Ca/Mmg ratio: 1.76
- Culture medium different from test medium: yes
- Intervals of water quality measurement: Once prior to beginning for the above paramters
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light - 8 h dark, with transition periods of 30 minutes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50, LC 100, NOEC (mortality = 96h LC0), NOEC (condition after 96 h).
Condition: Swimming behaviour, colour, respiratory function, any other morphological or visual behavioural criterion after 24 h, 48 h, 72 h and 96 h.
TEST CONCENTRATIONS
- Test concentrations: 1.0, 1.8, 3.2, 5.6, 10 g/l - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks:
- : Swimming behaviour, colour, respiratory function, any other morphological or visual behavioural criterion
- Remarks on result:
- other: NOEC (condition)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.8 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- (= 96h LC0)
- Remarks on result:
- other: One animal was dead at 1.8 g/l, but because mortality (in the control) is allowed according to OECD 203, this concentration is recorded as the 96h NOEC, with regard to mortality as criterion.
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 5.6 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.8 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval: 2.3 - 3.3 g/l
- Details on results:
- It is likely that the results of the test are seriously affected by the high pH value.
- Behavioural abnormalities: At concentrations of 1.8 g/l and 3.2 g/l L-arginine slow swimming fishes, fishes with disturbed equilibrium, fishes swimming near the surface of the test vessel, fishes with a darker colour and fishes making nervous movements were observed.
- Observations on body length and weight: none
- Other biological observations: none
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test substance appeared to be completely dissolved at all concentrations tested at the beginning of the test (visually assesed). After 24 h undissolved material was observed on the bottom of the vessel at all concentrations tested; the amount of undissolved material increased with the concentration.
- Effect concentrations exceeding solubility of substance in test medium: see above
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of L-arginine to fish was determined according to OECD guideline 203.
The test results were: LC50 = 2.8 g/l; LC 100 = 5.6 g/l; NOEC (mortality) = 1.8 g/l; NOEC (condition) = 1.0 g/l.
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