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EC number: 834-791-6 | CAS number: 1310681-27-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- other information
- Study period:
- 2019-03-25 to 2019-04-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [2,2,6,6-tetramethyl-4-(trimethylazaniumyl)piperidin-1-yl]oxidanyl chloride
- EC Number:
- 814-375-0
- Cas Number:
- 67036-09-3
- Molecular formula:
- C12H26N2OCl
- IUPAC Name:
- [2,2,6,6-tetramethyl-4-(trimethylazaniumyl)piperidin-1-yl]oxidanyl chloride
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 50.0, 25.0, 12.5, 6.25 mg/L and control
- Sampling method: Samples (1mL) from all test substance concentrations and control were taken at test start and test end (48 h).
- Sample storage conditions before analysis: deep frozen. All solutions were processed at room temperature allowing enough time to equilibrate.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 100 mg/L was prepared by weighing the test substance on a weighing scoop and transfering to a flask. Test medium (Elendt M4) was added up to a volume of 1000 mL and the solution was homogenised by shaking.
Lower test concentrations were prepared by dillution of the appropriate solution.
- Controls: Test medium without test substance
- Evidence of undissolved material: The stock solution (100 mg/L) was clear and slightly orange, lower concentrations were clear and transparent.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna, water flea
- Strain/clone: Clone V
- Source: Federal Evnironment Agency, Berlin/Germany
- Age at study initiation (mean and range, SD): less than 24 h
- Stage and instar at study initiation: freshly hatched daphnids less than 24 h were used for the test
- Method of breeding: continiously bred in the laboratory.
-Feeding during test: no
ACCLIMATION
- Acclimation conditions: same
- Type and amount of food: green algae (Desmodesmus subspicatus)
- Feeding frequency: at least three times per week, ad libitum
- Method for preparation of early instars: A pipette was used to separate the young daphnids from the adults.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 140 - 250 mg/L (as CaC03), corresponding to 7.8 - 14°dH
- Test temperature:
- 18.9-20.5°C
- pH:
- 7.34.-7.95
- Dissolved oxygen:
- >= 8.3 mg/L
- Nominal and measured concentrations:
- nominal concentrations: 100, 50.0, 25.0, 12.5, 6.25 mg/L and control. Measured concentration were in the range of 99 to 107% in new medium and in the range of 109 to 115 in the aged medium. Therefore, the nominal concentrations were used.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vessl (100 mL volume)
- Type: covered with glass plate
- Material, size, headspace, fill volume: Glass vessel (100 mL volume), filled up with >= 50 mL test medium.
- Volume of solution: 50 mL
- Aeration: no
- Renewal rate of test solution: static test, no renewal
- No. of organisms per vessel: 5 per vessel
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
- Biomass loading rate: ~10 mL for each daphnid
- 1 additional replicate per test substance concentration without organisms for physico-chemical analysis
TEST MEDIUM / WATER PARAMETERS
Elendt M4 medium according to guideline
OTHER TEST CONDITIONS
- Adjustment of pH: not during study period, at test start the pH-value of the control was 7.34.
- Photoperiod: 16 h lgiht /8 h darkness daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation, behavioural changes at 24 h and 48 h. Test temperature and pH-values, oxygen concentration at 0 h, 24 h, 48 h
RANGE-FINDING STUDY
- Test concentrations: 100, 10, 1.0, and 0.1 mg/L
- Results used to determine the conditions for the definitive study: The result of the pretest were used to derive the test substance concentrations of the main study. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Substance specific analytic showed stable test substance concentration in aged medium. Therefore, the effect levels are based on the nominal concentration.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Substance specific analytic showed stable test substance concentration in aged medium. Therefore, the effect levels are based on the nominal concentration.
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 24 and 48 hours of exposure no immobilisation was observed in the control and up to and including the highest test item concentration of 100 mg/L. No behavioural changes could be observed during the course of the study.
The measured content of test substance was between 99 % and 107 % of nominal in the fresh samples with a mean initial concentration of 104 % of nominal. In the aged samples the measured content was between 109 % and 115 % of nominal with a mean measured concentration of 112 % of nominal. Therefore, toxicological endpoints were evaluated using the nominal concentrations. - Results with reference substance (positive control):
- EC50 (24 h) between 0.6 to 2.1 mg/L
- Reported statistics and error estimates:
- All toxicological endpoints were evaluated using nominal concentrations. The values for EC50 could not be determined statistically. The NOEC has been based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation (≤ 10 % immobilisation).
Any other information on results incl. tables
Results of the test, 48 h values
|
Nominal test item concentration [mg/L] |
|||||
|
Control |
6.25 |
12.5 |
25.0 |
50.0 |
100 |
|
Immobilised daphnids after 48 h |
|||||
Replicate 1 |
0 |
0 |
0 |
0 |
0 |
0 |
Replicate 2 |
0 |
0 |
0 |
0 |
0 |
0 |
Replicate 3 |
0 |
0 |
0 |
0 |
0 |
0 |
Replicate 4 |
0 |
0 |
0 |
0 |
0 |
0 |
Σ |
0 |
0 |
0 |
0 |
0 |
0 |
% |
0 |
0 |
0 |
0 |
0 |
0 |
Determined concentration of TMA-TEMPO
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- In this study the control immobilisation was 0 %. The dissolved oxygen concentration at the end of the test was ≥ 8.3 mg/L.
- Conclusions:
- After 24 and 48 hours of exposure no immobilisation was observed in the control and up to and including the highest test item concentration of 100 mg/L. The EC50 (48 h) was supposed to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
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