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Diss Factsheets
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EC number: 205-758-8 | CAS number: 150-38-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- GLP compliance:
- no
- Remarks:
- (Published) Study not performed for REACH purposes. Therefore, GLP-compliance was not an issue.
- Analytical monitoring:
- not specified
- Details on sampling:
- no data
- Vehicle:
- no
- Details on preparation and application of test substrate:
- EDTA was applied in deionized water solution into artificial soil (35 % dry weight moisture content).
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- no data available
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- no data available
- Test temperature:
- 20 °C
- pH:
- no data available
- Moisture:
- 35 % of maximum holding capacity
- Details on test conditions:
- Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.
- Nominal and measured concentrations:
- 0, 0.1, 1.0, 10, 100, 1000 and 10000 mg/kg dry weight soil.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 156.46 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 136.01 - 179.98 mg/kg soil dw
- Results with reference substance (positive control):
- no data available
- Reported statistics and error estimates:
- Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests. - Validity criteria fulfilled:
- not specified
- Conclusions:
- The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156.46 mg/kg dry weight soil.
- Executive summary:
The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156.46 mg/kg dry weight soil.
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to the Analogue Approach Justification document provided in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 156.46 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 136.01 - 179.98 mg/kg soil dw
Referenceopen allclose all
Description of key information
LC50 (14 d) of 156.46 mg/kg soil dw (OECD 207)
Key value for chemical safety assessment
Additional information
Since no studies investigating the toxicity trisodium hydrogen EDTA (CAS 150-38-9) to soil macroorganisms are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to structurally related EDTA species was conducted. This read-across is justified in detail in the overall summary (IUCLID Section 6.3) and within the analogue justification in IUCLID Section 13.
For the assessment of toxicity to terrestrial organisms data from the source substance edetic acid (CAS 60-00-4) were used.
The available study was performed according to OECD 207 (Edwards et al., 2009). Eisenia fetida was exposed to EDTA in an artificial soil. Ten species were added to each test vessel and four replicates were prepared for each concentration. Concentrations of 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/kg dw were tested including a control. After 14 d the mortality at the different test vessels was recorded and a LC50 (14 d) of 156.46 mg/kg soil dw resulted.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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