Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 271-676-4 | CAS number: 68603-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication which meets basic scientific principles Data from ciliate growth inhibition tests, preferably with the species Tetrahymena are also relevant for the risk assessment for STPs.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- TETRATOX assay: Tetrahymena pyriformis population growth impairment
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Nonanoic acid is one of the main constituents of the target UVCB substance
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- - Test organism: Tetrahymena pyriformis (strain GL-C)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 40 h
- Test temperature:
- 27 ± 1 °C
- Nominal and measured concentrations:
- five concentration levels
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 mL Erlenmeyer flasks, 50 mL semi-defined medium
- No. of organisms per vessel: ca. 2,500 cells/mL in log-growth phase
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2 + 1 blank
- No. of tests per substance: 3
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- growth impairment: population density is measured spectrophotometrically
TEST CONCENTRATIONS
- Range finding study performed
STATISTICAL EVALUATION
- 50 % inhibitory growth concentration in mg/L (IGC50)
- IGC50: Probit analysis using the percent control-normalized absorbance as the dependent variable and the toxicant concentration in mg/L as the independent variable - Duration:
- 40 h
- Dose descriptor:
- IC50
- Effect conc.:
- 71 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: original value reported: -log (IGC50) = 0.3509
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- Growth inhibition of Tetrahymena pyriformis: IGC50 (40 h): 71 mg/L
Nonanoic acid is one of the main constituents of the target UVCB substance. - Executive summary:
The growth inhibiting effect of nonanoic acid on the ciliate Tetrahymena pyriformis was determined using the TETRATOX assay. The resulting IGC50 (40 h) of 71 mg/L indicates a low toxicity of nonanoic acid to microorganisms (ciliates).
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1979
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- abstract
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
Bacillus subtilis was tested with different substances and the optical densitiy measured after 1 h to calculate the inhibition.- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Inoculum was placed into plastic tubes or Erlenmeyer flasks which already contained the test substance
- Controls: 2 with solvent, 2 with distilled water
- Chemical name of vehicle: Ethanol, dimethylsulfoxide, sodium hydroxide or distilled water
Test organisms- Test organisms (species):
- Bacillus subtilis
- Details on inoculum:
- Pretreatment: Plated with tryptose blood agar base (33 g/L) were inoculated from single colonies of Bacillus subtilis and incubated for 7 h. Afterwards the cells were supended and diluted at different low titers into three flasks containing the MCV medium and shaken at room temperature overnight. If the cultures had not grown overnight beyond an optical density at 600 nm (OD600) of 1.0 it was not used. The cultures reaching 1.0 were utilised for the test.- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 60 h
- Hardness:
- Not specified
- Test temperature:
- Not specified
- pH:
7.2, adjusted with KOH- Dissolved oxygen:
- Not specified
- Salinity:
- Not added
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Not specified
- Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic tubes or Erlenmeyer flasks
- Aeration: Tubes were aerated.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MCV medium contains 1.4% K2HPO4 , 0.6% KH2PO4, 0.2% MgSO4 * 7 H2O, 0.1% sodium citrate, 25 µg/mL L-tryptophan, 10 µg/mL L-methionine and 1 % vitamin-free casein hydrolysate
OTHER TEST CONDITIONS
- Adjustment of pH: with KOH to 7.2
EFFECT PARAMETERS MEASURED: optical density at 600 nm after 60 min in the plastic tubes and after 15 or 20 min in the Erlenmeyer flasks- Reference substance (positive control):
- no
- Duration:
- 60 min
- Dose descriptor:
- EC50
- Effect conc.:
- 1.88 mmol/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Growth inhibition of Bacillus subtilis: EC50 (60 h): 1.88 mmol/l
Octanoic acid is one of the main constituents of the target UVCB substance. - Executive summary:
The growth inhibiting effect of octanoic acid on Bacillus Subtilis was determined. The resulting EC50 (60 h) of 1.88 mmol/L indicates a low toxicity of octanoic acid to microorganisms
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not specified
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- abstract
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Six different bacteria were anaerobically incubated and the turbitidy measured to determine inhibition on growth.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution was prepared by dissolving measured quantities of the test substance in absolute ethanol. First a 250 mM stock solution was prepared and then further diluted with ethanol to 200, 175, 150, 125, 100, 75, 50, 25, 10, 5 and 2.5 mM.
- Controls: Since the test compund produces culture turbidity, a dilution series with medium and test compound without inoculum were prepared.- Test organisms (species):
- other: Bifidobacterium bifidum type a, Bifidobacterium bifidum type b, Bifidobacterium infantis sp infantis type a, Bifidobacterium infantis sp infantis type b, Escherichia coli, Salmonella typhimurium
- Details on inoculum:
- Preparation of inoculum for exposure: The PYG medium was dispensed into a 500 mL screw-capped bottle and sterilised at 121 °C for 15 min. While cooling, 3.0 mL were delivered into metal capped culture tubes which were inoculated with 0.03 mL of the substance stock solutions (2.5 - 20 mM). The culture tubes were placed in an anaerobic cabinet and maintained at 37°C over night. Oxygen free gas (20% nitrogen in hydrogen) was supplied.
Study design- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 29 h
- Hardness:
- Not specified
- Test temperature:
- 37 °C
- pH:
4.5 - 6.6, the pH was measured in the highest and lowest substance concentrations.- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 200, 175, 150, 125, 100, 75, 50, 25, 10, 5 and 2.5 mM
- Details on test conditions:
TEST SYSTEM
- Test vessel: culture tubes
- No. of vessels per control (replicates): one series (2.5 - 200 mM test substance) with test substance without inoculum to determine turbidity
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The PYG media (Petone-yeast extract glucose) with 1% glucose, was prepared and dispersed anaerobically and sterilised. Anaerobic culture counts were performed on PYG-agar roll tubes.
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: turbidity after 29 h- Reference substance (positive control):
- no
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 80 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other:
- Remarks:
- B. Bifidum type a
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other:
- Remarks:
- B. Infantis sp. Infantis type a
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other:
- Remarks:
- B. Infantis sp. Infantis type b
- Duration:
- 29 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0 other: nmol
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- S. typhimurium
- Details on results:
- No 50 % inhibition on bacterial growth was achieved on E. coli and B. bifidum type b.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- No 50 % inhibition on bacterial growth was achieved on E. coli and B. bifidum type b.
- Endpoint:
- activated sludge respiration inhibition testing
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant
- Justification for type of information:
- In consideration of the results obtained from the study according to OECD 301B (substance ready biodegradable) no toxicity is expected on aquatic microorganisms at the expected concentration
Referenceopen allclose all
Description of key information
Disturbances in sewage treatment plants are not to be expected when the target UVCB substance is released in appropriate amounts. The substance is readily biodegradable.
Also the available data on the substance main constituents are in agreement with this conclusion.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 32 mg/L
Additional information
The results of the OECD 301B study (Tosin, 2018) indicate that
a) Carboxylic acid, C5-9 is readily biodegradable,
b) disturbances in sewage treatment plants are not to be expected when Carboxylic acid, C5-9 is released in appropriate amounts, and
c) inhibitory effects of Carboxylic acid, C5-9 to sewage sludge bacteria was not observed at a concentration of 32 mg/L of Carboxylic Acids, C5 -9
In addition, the growth inhibiting effect of nonanoic acid on the ciliate Tetrahymena pyriformis was determined: IGC50 (40 h) = 71 mg/L (Schultz, 2006).
Supportive information is withdrawn from short abstracts of two publications. In the first study (Freese, 1979) conducted with Bacillus subtilis, a EC50 of 0.25 mmol/L was obtained after 60 min. In the second study (Powell and May, 1981) with six different bacterial strains incubated anaerobically, different sensitivity of the various organisms to decanoic acid were observed. However, due to limited documentation and consequently limited information, these two literature studies were rated as not assignable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.