Phosphoric acid, chromium(3+) salt (1:2.5)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30.05.2018-17.09.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

LAUS GmbH

Test material

Test material information

Constituent 1

Reference substance name:

Phosphoric acid, chromium(3+) salt (1:2.5)

EC Number:

946-354-8

Molecular formula:

not applicable for UVCB

IUPAC Name:

Phosphoric acid, chromium(3+) salt (1:2.5)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Berlin
- Justification for species other than prescribed by test guideline:
- Source: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD):
- Feeding during test: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.502 mmol/L

Test temperature:

20+/-2 °C

pH:

6.2-7.9

Dissolved oxygen:

8.0-9.0 mg/L

Nominal and measured concentrations:

8.4 / 18 / 40 / 84 / 182 mg/L (nominal)
4.6+/-1 /10 / 22 / 46 / 100 mg/L (active ingeredient, without water content of 55%)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Type: open
- Material, size, headspace, fill volume: 50mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates):4

TEST MEDIUM / WATER PARAMETERS
Medium M4-Medium (recipe of ELENDT)
Dilution water (Daphnia medium) with the following specification was used (stated in SOP 11800201)
Parameter Concentration in mg/L
CaCl2*2H2O 293.80
MgSO4*7H2O 123.30
NaHCO3 64.80
KCl 5.80
Resulting hardness in mmol/L: 2.502
Resulting hardness in mg CaCO3/L: 250


RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 161.51 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

measured concentration based on measured Cr content in unfiltered solution

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 88.83 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Remarks:

measured concentration based on measured Cr content in unfiltered solution, corrected for water content

Basis for effect:

mobility

Details on results:

In filtered solution 8experiment 1 and 2) the concentrations were lower: Exp. 1 EC50 (48h) >10.68 mg/L test mat. and Exp. 2 EC50 (48h) >9.59 mg/L test mat.

Results with reference substance (positive control):

Potassium dichromate K2Cr2O7 was used as positive control in a current reference study. The 24h-EC50 value was determined as 1.9 mg/L, lying within the demanded range of 0.6 – 2.1 mg/L.

Reported statistics and error estimates:

Results was calculated with the help of validated software (Microsoft Excel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC 50 (48 h) for Daphnia magna was >161.51 mg/L (>88.83 mg/L active ingredient).

Executive summary:

The toxicity of the test substance to Daphnia magna was investigated in a test conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test; 2004), EU Method C.2 (Acute Toxicity for Daphnia; 2008). At a test substance concentration of 161.51 mg/L measured (= 88.83 mg/L active ingr.) 20% immobilisation to daphnids could be detected (48 h EC50> 161.51mg/L measured or 88.83 mg/L active ingr. 100 mg/L).