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EC number: 601-485-7 | CAS number: 117570-93-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07-nov-2008 to 21-nov-2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD and/or EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[2-(carboxymethyl)phenoxy]-3,4-dimethylbenzoic acid
- EC Number:
- 601-485-7
- Cas Number:
- 117570-93-1
- Molecular formula:
- C17 H16 O5
- IUPAC Name:
- 2-[2-(carboxymethyl)phenoxy]-3,4-dimethylbenzoic acid
- Details on test material:
- - Name of test material (as cited in study report): ASA404 C7
- Substance type: Off white powder
- Physical state: Solid
- Analytical purity: 99.9%
- Lot/batch No.: 072301
- Expiration date of the lot/batch: 18 March 2009
- Stability under test conditions: Stable
- Storage condition of test material: At room temperature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Method- Since ASA404 C7 was hardly soluble in water test concentrations were prepared separately in Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA). One litre test bottles were filled with 250 ml of test substance mixtures with initial loading rates twice as high as the final test concentration. These mixtures were stirred in closed bottles for 25-27 hours. Subsequently, 16 ml synthetic sewage feed, 200 ml sludge and Milli-RO water up to 500 ml were added resulting in the final loading rates (see also section 6.4). Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring.
- Controls: blank and reference substance
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.8 and 4.4 g/l of sludge, as used for experiment 1 and 2, respectively). The pH was 7.8 on the day of testing in both experiments. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- Oxygen consumption was measured and recorded for approximately 10 minutes after the 3 hours treatment period.
Test conditions
- Test temperature:
- first study: 19.1 - 19.6°C
second study: 19.0 - 20.6°C - pH:
- first study: 6.3 - 8.1
second study: 6.1 - 8.0 - Dissolved oxygen:
- Oxygen consumption
first study: 14 - 17 mg O2/l/h
second study: 11 - 54 mg O2/l/h
- Nominal and measured concentrations:
- First study: nominal concentration: 1000 mg/l
Second study: nominal concentrations: 100, 180, 320, 560 and 1000 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: All glass, approximately 300 ml oxygen bottles (measuring) and 1 litre test bottles (exposure)
- Aeration: during exposure with clean, oil-free air
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: 1.8 g/l suspended solids
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO)
EFFECT PARAMETERS MEASURED :
-The respiration rate from each vessel, in mg O2/l/hr, was calculated from the linear part of the respiration curve, which was generally between 2.5 and 6.5 mg O2/l.
TEST CONCENTRATIONS
- First study: Test concentrations: 1000 mg/l. Results used to determine the conditions for the definitive study: yes
- Second study: Spacing factor for test concentrations: geometric progression with a factor 1.8 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In the first experiment 62 and 69% inhibition of the respiration rate of the sludge was recorded at a loading rate of 1000 mg ASA404 C7 per litre.
In the second experiment no significant inhibition of the respiration rate of the sludge was recorded up to a loading rate of 180 mg/l (based on nominal concentrations). At a loading rate of 320 and 560 mg/l very slight inhibition (13%) of respiration rate of the sludge was recorded. Whereas, 80% inhibition of the respiration rate of the sludge was recorded at a loading rate of 1000 mg/l.
The EC50 was estimated to be at a loading rate of 770 mg/l (based on nominal concentrations). - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The EC50 of 3,5-dichlorophenol was 7.6 mg/l - Reported statistics and error estimates:
- The EC50, EC20 and EC80 for the test/reference substance, was determined using linear regression analysis.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- It is concluded that this test is valid and that significant inhibition of respiration rate of the sludge was recorded at and above a loading rate of 320 mg/l (based on nominal concentrations).
The EC50 was estimated to be at a loading rate of 770 mg/l (based on nominal concentrations).
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