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EC number: 603-101-3 | CAS number: 125962-78-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-11-08 to 2018-01-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Based on the results of the preliminary test it could concluded that the heterotrophic respiration as well as the nitrification are not inhibited. Therefore, the definitive study was carried out with the concentration of 1000 mg/L in 3 replicates (limit test to determine the NOEC). The concentration was weighed out directly in the Erlenmeyer flasks. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Activated sludge, microorganisms from a domestic waste water treatment plant
- Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant of Hildesheim, Germany
- Pretreatment: The Sludge was washed twice with chlorine free tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 10 %. The Sludge was used within 24 h after sampling. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20°C
- pH:
- pH of activated sludge: 7.49
pH of synthetic waste water: 7.27 - Dissolved oxygen:
- Control samples : 6.99 - 7.80 mg/O2/L;
Test item sample: 5.72 – 5.74 mg O2/L - Nominal and measured concentrations:
- Nominal conc.: 1000 mg/L;
Measured conc.: not determined - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flasks
- Type: open
- Aeration: Permanent, to keep the dissolved oxygen concentration above 60 - 70 % Saturation and to maintain the Sludge flakes in Suspension.
- No. of vessels per concentration (replicates): main study: 3
- No. of vessels of positive control (replicates): 3
- No. of vessels of control (replicates): 6
- Sludge concentration (weight of dry solids per volume): 2.80 g/L (corresponding to 1.40 g/L suspended solids in the test vessels)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
EFFECT PARAMETERS MEASURED: The oxygen uptake rates of all samples will be determined following an incubation period of 3 h.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- Copper-(II)-sulphate pentahydrate
EC50 = 104 mg/L - The mean specific oxygen uptake rate of the control replicates was 20.5 mg O2/g.h (validity criterion: should be > 20 mg O2/g.h).
- The coefficient of variation of the oxygen uptake rates in the control replicates was 2.9 % (validity criterion: < 30 %).
- The EC50 of the reference item was 104 mg/L and therefore in the range of 53 - 155 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item is ≥ 1000 mg/L. The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L.
- Executive summary:
The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions in a study according to OECD TG 209. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of activated sludge was measured in the presence of various concentrations of the test item after an incubation period of 3hours. The test was carried out under static conditions with the limit test item concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates was -1%. In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 104 mg/L. The validity criteria of the test guideline were fulfilled. The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L. Thus, the NOEC of the test item is determined to ≥ 1000 mg/L.
Reference
Validity Criteria
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration
Table 1: Oxygen Uptake Rates, Inhibition of the Respiration of Control Replicates
| Replicate | Oxygen Concentration* [mg O2/L] | Oxygen Uptake Rate R [mg O2/(L*h)] | Specific Respiration Rate Rs [mg O2/g*h] |
Control | 1 | 7.67 | 28.0 | 20.0 |
2 | 7.62 | 27.3 | 19.5 | |
3 | 7.56 | 28.6 | 20.4 | |
4 | 7.09 | 29.8 | 21.3 | |
5 | 6.99 | 29.3 | 20.9 | |
6 | 7.80 | 29.0 | 20.7 | |
| Mean value | 28.7 | 20.5 | |
Standard deviation | 0.83 |
| ||
CV [%] | 2.9 |
|
Table 2: Oxygen Uptake Rates and Inhibition of the Respiration of the Test Item Concentration
Test Item Concentration [mg/L] | Replicate | Oxygen Concentration* [mg O2/L] | Oxygen Uptake Rate R [mg O2/(Lxh)] | Inhibition [%] | Mean Inhibition [%] |
1000 | 1 | 5.74 | 29.2 | -2 |
|
2 | 5.81 | 28.9 | -1 | -1 | |
3 | 5.72 | 28.9 | -1 |
|
*) determined at Start of the measurement
CV = Coefficient of Variation
Table 3: NOEC of the test item
| Test item Concentration [mg/L] |
NOEC* | > 1000 |
Description of key information
The NOEC of the test item is ≥ 1000 mg/L. The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L (reference 6.1.7-1).
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions in a study according to OECD TG 209. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of activated sludge was measured in the presence of various concentrations of the test item after an incubation period of 3hours. The test was carried out under static conditions with the limit test item concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates was -1%. In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 104 mg/L. The validity criteria of the test guideline were fulfilled. The test item caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L. Thus, the NOEC of the test item is determined to ≥ 1000 mg/L.
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