Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 701-266-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Radiolabelling:
- no
- Details on study design: HPLC method:
- EQUIPMENT/MOBILE PHASES
Instrument: Acquity UPLC system (Waters, Milford, MA, USA)
Detector: Acquity UPLC TUV detector (Waters) and ZQ 2000 mass spectrometer (Waters)
Column*: Hypersil BDS-CN, 100 mm 2.1 mm i.d., dp = 3 µm (Thermo Fisher Scientific, Waltham, MA, USA)
Column temperature: 35°C
Mobile phase: 0.1% formic acid in 55/45 (v/v) methanol/water
Flow: 0.2 ml/min
Injection volume: 10 µl
UV detection: 210 nm
MS detection:
- Ionisation source: ESI+
- Cone voltage: 28 V
- Acquisition: Full scan m/z 50 - 250 amu; MRM m/z 233 amu (test substance); MRM m/z 147 amu (structural homologue)
* Analyses were also performed without column in the analytical system (autosampler was connected to the detectors via a by-pass).
REFERENCE SUBSTANCES
2,4-DDT (97.2%, Supelco, Bellefonte, PA, USA);
TETA, structural homologue of the test substance:
- Name: Triethylenetetramine (TETA)
- Molecular formula: C6H18N4
- Molecular weight: 146.23
- Substance type: Clear colourless liquid
- Physical state: liquid
- Analytical purity: 96.2%
- Lot/batch No.: P0632
- Expiration date of the lot/batch: 14 August 2010
- Stability under test conditions: Stable
- Storage condition of test material: At room temperature in the dark under nitrogen
Log Koc structural homologue: 3.5
Log Koc 2,4-DDT: 5.63
DETERMINATION OF RETENTION TIMES
Reference substance solution: A 1.05 g/l stock solution of 2,4-DDT in methanol was used. The stock solution was diluted to obtain an end solution of 0.1% formic acid in 55/45 (v/v) methanol/water.
Structural homologue solution: A 1148 mg/l stock solution of the structural homologue was prepared in water. The stock solution was diluted with 0.1% formic acid in 55/45 (v/v) methanol/water. The final concentration of the structural homologue solution was 100 mg/l.
Test substance solution: A 1188 mg/l stock solution of the test substance was prepared in water. The stock solution was diluted with 0.1% formic acid in 55/45 (v/v) methanol/water. The final concentration of the test substance solution was 100 mg/l.
REPETITIONS
- Number of determinations: 2
EVALUATION
Retention time of the test substance was compared with the retention time of its structural homologue. - Type:
- log Koc
- Value:
- > 3.5 dimensionless
- Temp.:
- 35 °C
- Details on results (HPLC method):
- The retention time of 2,4-DDT (log Koc = 5.63) was 5.1 minutes.
In the chromatogram of the first injection of the structural homologue no peak could be detected. Based upon its log Koc value of 3.5 (Koc = 3162) the substance was expected to elute before 2,4-DDT. In the chromatogram of the second injection and of the first injection of the corresponding blank, broad peaks were detected which were due to the previous injections. This indicates that the structural homologue eluted from the column with a retention time of approximately 19 minutes.
In the chromatograms of the test substance solution, no peaks were detected. In the chromatograms of the blank solutions injected immediately after the test substance solutions, no test substance peak could be detected either.
To demonstrate that the test substance was applied, the solutions were also analysed in the by-pass mode (i.e. without the analytical column).The structural homologue and the test substance eluted in a similar way through the analytical system (peak maximum at a retention time of 1.1 minutes), whereas 2,4-DDT eluted unretained (peak maximum at 0.2 minutes). Based on this information, the Koc of the test substance should be compared with the Koc of the structural homologue, and not with 2,4-DDT.
Because the test substance was not eluted from the column within 60 minutes after the first injection, it can be safely concluded that the log Koc of the test substance is higher than the log Koc of the structural homologue. - Validity criteria fulfilled:
- yes
- Conclusions:
- The log Koc value of the substance was determined to be > 3.5.
Reference
Description of key information
The substance is adsorptive (Log Koc > 3.5)
Key value for chemical safety assessment
- Koc at 20 °C:
- 3 162
Additional information
The log Koc of the substance was estimated in a guideline study according to OECD 121. The log Koc was estimated by HPLC. The retention time of the test substance was compared with the retention time of the structural homologue substance Triethylenetetramine. The log Koc of Triethylenetetramine was determined according to OECD guideline 106 (log Koc: 3.5). A log Koc of >3.5 for the test substance was assumed due to the longer retention time of the test substance compared to the structural analogue.
Based on the results, the substance is considered adsorptive.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.