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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Avena Sativa leaf/stem extract
- IUPAC Name:
- Avena Sativa leaf/stem extract
Constituent 1
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Source: The algae were supplied by the SAG: Collection of Algal Cultures, Inst. Plant Physiology, and University of Göttingen, GERMANY. Cultured under standardised conditions (see OECD 201) in the Ecotoxicological Laboratory of Citoxlab Hungary Ltd.
Justification of species: The species of Pseudokirchneriella subcapitata used, being a fast-growing species, is convenient for culturing and testing and is a recommended species by relevant guidelines.
Breeding conditions: Stock cultures are small algal colonies that are inoculated onto agar regularly. These are transferred to fresh agar medium at least once every two months and are maintained under standardised conditions according to the test guidelines.
The pre-culture is intended to give a quantity of algae suitable for the inoculation of test cultures. The pre-culture was prepared with the OECD algal growth medium, incubated under the same conditions as the test and used when still growing exponentially, after an incubation period of three days. When the algal cultures contain deformed or abnormal cells, they were discarded.
Study design
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- The exposure time was 72 hours. The test was started (0 hours) by inoculation of a biomass of approximately 104 algal cells per mL test medium.
Test conditions
- Test temperature:
- Culture temperature was checked at the beginning of the experiment and each day thereafter in a flask filled with water, in the climatic chamber. In addition, water temperature was continuously measured (with a min/max thermometer) within the climate chamber. The temperature was in the range of 22.4 – 22.7 °C measured in the flask and between 22.1 and 23.1 °C measured within the climate chamber.
- pH:
- The pH was checked at the beginning and at the end of the test in each test vessels. The pH of the control medium was not increased by more than 1.5 units during the test. The range of the pH was 7.28 – 8.80 during the experiment.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- Based on the nominal loading rate (WAF):
The 72h EbL50 value (biomass): > 100 mg/L nominal loading rate WAF
The 72h ErL50 value (growth rate): > 100 mg/L nominal loading rate WAF
The 72h EyL50 value (yield): > 100 mg/L nominal loading rate WAF
The 72h No Observed Effect Loading Rate (NOELR):
100 mg/L nominal loading rate WAF
The 72h Lowest Observed Effect Loading Rate (LOELR):
> 100 mg/L nominal loading rate WAF
Based on the measured test item concentration:
The 72h EbC50 value (biomass): > 100 mg/L (measured)
The 72h ErC50 value (growth rate): > 100 mg/L (measured)
The 72h EyC50 value (yield): > 100 mg/L (measured)
The 72h No-Observed Effect Concentration (NOEC): 100 mg/L (measured)
The 72h Lowest Observed Effect Concentration (LOEC): > 100 mg/L (measured)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of EXT. AQ. CONC. PLANTULE AVOINE was assessed on algal growth using the unicellular green alga Pseudokirchneriella subcapitata (formerly known as Selenastrum capricornutum), over an exposure period of 72 hours.
Under the conditions of this algal growth inhibition test the calculated endpoints for the effect of EXT. AQ. CONC. PLANTULE AVOINE were the following:
Based on the nominal loading rate (WAF):
The 72h EbL50 value (biomass): > 100 mg/L nominal loading rate WAF
The 72h ErL50 value (growth rate): > 100 mg/L nominal loading rate WAF
The 72h EyL50 value (yield): > 100 mg/L nominal loading rate WAF
The 72h No Observed Effect Loading Rate (NOELR):
100 mg/L nominal loading rate WAF
The 72h Lowest Observed Effect Loading Rate (LOELR):
> 100 mg/L nominal loading rate WAF
Based on the measured test item concentration:
The 72h EbC50 value (biomass): > 100 mg/L (measured)
The 72h ErC50 value (growth rate): > 100 mg/L (measured)
The 72h EyC50 value (yield): > 100 mg/L (measured)
The 72h No-Observed Effect Concentration (NOEC): 100 mg/L (measured)
The 72h Lowest Observed Effect Concentration (LOEC): > 100 mg/L (measured)
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