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EC number: 812-958-4 | CAS number: 1078712-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 10, 2017 to July 31, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 001
- Cas Number:
- 1078712-76-1
- Molecular formula:
- C23-27H49-59N (Substance is a UVCB)
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the test solutions were collected at approximately 0 and 72 hours for analytical
verification of the test concentrations. At test initiation (0 hour), samples were collected for each
treatment and control group prior to distribution into test chambers. At test termination (72 hours), the
biological replicates from each respective treatment and control group were pooled and then sampled. At
each sampling interval 5.0 mL of test solution was transferred into glass scintillation vials containing
5.0 mL of 0.2% formic acid in methanol.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Individual WAF solutions were prepared by mixing a calculated amount of test substance in
freshwater AAP medium at nominal loading rates of 0.26, 0.64, 1.6, 4.0, 10, and 25 mg/L. Loading rates
were not corrected for percent active ingredient in the test substance. The negative control solution
consisted of one aspirator bottle containing 4000 mL of freshwater AAP medium without test substance
added. The negative control bottles were subjected to identical mixing procedures as the Amines,
(2-ethylhexyl)(hydrogenated tallow alkyl)methyl test solutions.
Glass aspirator bottles were filled with 4000 mL of freshwater AAP medium measured with a
2000mL glass volumetric flask. Required mass of test substance for each solution was weighed into tared
30 mL glass beakers using an analytical balance and rinsed into 4000 mL glass aspirator bottles with a
portion of the freshwater AAP medium previously removed from each bottle. The actual mass of test
substance weighed for the nominal 0.26 and 0.64 mg/L treatment groups resulted in actual concentrations
of 0.25 and 0.65 mg/L. The discrepancy was caused by the precision of the analytical balance and the
differences between the actual and nominal values were considered to be negligible (0.01 mg/L). Water
accommodated fractions were stirred with a Teflon-coated stirbar and magnetic stir plate for twenty-three
hours and were allowed to settle for one hour prior to use. While stirring, a minimal vortex depth was
maintained. Precursory work conducted indicated that lower energy mixing produced better analytical
recoveries. After settling, the test solutions were decanted from mid-depth via tubing on each aspirator
bottle. Glass wool was inserted into the tubing on each bottle to avoid transferring any undissolved test
substance or particulates into the test solutions. After mixing, all test solutions appeared clear and
colorless; however, test substance was visible on the surface of the 0.26, 0.64, 1.6, 4.0, 10, and 25 mg/L
test solutions at the end of the settling period.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 +/-2 C
- pH:
- 7.5 +/- 0.1
- Nominal and measured concentrations:
- Nominal WAF Loading Rate
Negative Control
0.26 mg/L
0.64 mg/L
1.6 mg/L
4.0 mg/L
10 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 0.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 0.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
Results of analyses to measure concentrations of Amines, (2-ethylhexyl)(hydrogenated tallow
alkyl)methyl in test solution samples collected during the study are presented in Table 2. Measured
concentrations of Amines, (2-ethylhexyl)(hydrogenated tallow alkyl)methyl in samples collected from the
negative control at 0 and 72 hours of exposure were <LOQ. Measured concentrations of Amines,
(2-ethylhexyl)(hydrogenated tallow alkyl)methyl in samples collected from all treatment groups at 0 and
72 hours of exposure were <LOQ.
The results of the study are based on nominal WAF loading rates. Water accommodated fractions
are used for poorly soluble materials and the high masses of test substance added are not fully dissolved
in the test medium as evidence by the particulates visible in all treatment groups at the end of the mixing
period. Nominal WAF loading rates were considered to be more applicable based on the concept of WAF
studies.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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