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EC number: 445-090-6 | CAS number: 5614-37-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 November 2002 - 18 January 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD and EC test guidelines, and in compliance with GLP; on this basis the data is considered reliable without restrictions.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Analytical purity: 99.9%
- Lot/batch No.: 020618 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10, 22, 46, 100, and 220 mg/L (nominal concentrations)
Test media were prepared daily. Samples were taken from the first and last batches of media, both at the time of preparation (0 hours and 72 hours), and from the expired medium (24 hours and 96 hours). - Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: The test substance was directly spiked into diluent water.
Preliminary investigations into the solubility and stability indicated that the test substance was volatile and readily lost from aqueous mixtures. Therefore, each aspirator was sealed with a tightly fitting lid top prevent loss of material. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Length at study initiation (length definition, mean, range and SD): mean = 5.7 cm; S.D. = 0.6 cm
- Weight at study initiation (mean and range, SD): mean = 3.11 g; S.D. = 0.92 g.
- Feeding during test: No
ACCLIMATION
- Acclimation period: From 12 September 2002 (n.b. preliminary test commenced 19 November 2002; main test commenced 13 January 2003)
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: fed to repletion with commercial fry pellets
- Feeding frequency: daily except for the 24 hour period before the test
- Health during acclimation (any mortality observed): Fewer than 1% in the 7 days before the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- 157 mg CaCO3/L
- Test temperature:
- 11 to 15°C
- pH:
- 7.1 to 7.3
- Dissolved oxygen:
- 7.3 - 7.5 mg O2/L
- Nominal and measured concentrations:
- Nominal: 10, 22, 46, 100, and 220 mg/L
Measured (mean of 4 samples analysed): 11, 23, 49, 103, 220 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass Duran aspirators
- Type (delete if not applicable): Sealed with a tightly fitting lid, and test vessels were completely filled to minimise headspace (to prevent loss of the volatile test material)
- Aeration: No supplementary aeration after the start of the test.
- Renewal rate of test solution (frequency/flow rate): Daily (every 24 hours)
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.95 g/L
OTHER TEST CONDITIONS
- Adjustment of pH: Not required
- Photoperiod: 16 h light / 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Absence of respiratory movement, and absence of response to physical stimulation of the caudal peduncle.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Approximately 2.2
- Range finding study
- Test concentrations:10, 100, and 220 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- Sublethal effects including hyperventilation, increased pigmentation, resting on the base of the vessel, swimming on the top of the vessel, and loss of equilibrium were seen across all test material concentrations including the control level. As these effects were seen in the control level as well as the treated levels, it is thought that they were related to the test conditions employed (specifically the use of sealed vessels) rather than the result of treatment with the test material.
- Mortality of control: One mortality in the control group was observed, however this was within the acceptability criteria for the test guideline.
By the end of the 96-hour exposure period, three fish died in the 46 mg/L level, and one fish died in the 100 mg/L level; as no deaths were recorded in the 220 mg/L level, the deaths were not seen to follow a dose relationship, and the deaths were not considered to be due to treatment with the test material. - Conclusions:
- The 96 hour LC50 for NZE to rainbow trout was greater than 220 mg/L.
The "no-observed effect concentration" (NOEC) for NZE in Rainbow trout was 220 mg/L.
Reference
Description of key information
Oncorhynchus mykiss, Rainbow trout, LC50 (96 hours) > 220 mg/L. NOEC = 220 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 220 mg/L
Additional information
A determination of the acute toxicity to fish of the substance was conducted (Huntingdon Life Sciences, 2003). The study was performed in accordance with EC method C1 and OECD test guideline 203, and in compliance with GLP. Groups of 7 rainbow trout (Oncorhynchus mykiss) were exposed to 0 (control group), 10, 22, 46, 100, or 220 mg/L the substance in dechlorinated water for 96 hours.
A number of fish died in two of the treated groups, however this was not attributed to the test material as no deaths were observed in the 220 mg/L level. Sublethal effects were also seen, but these were seen in the control group as well as the treated groups and so the effects were not considered to be related to treatment with the test material.
It was concluded that the 96 -hour LC50 in Rainbow trout was greater than 220 mg/L. The no-observed effect concentration was concluded to be 220 mg/L.
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