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EC number: 294-955-2 | CAS number: 91771-62-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Cyperus scariosus, Cyperaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07.02.2018-18.05.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyperus scariosus, ext.
- EC Number:
- 294-955-2
- EC Name:
- Cyperus scariosus, ext.
- Cas Number:
- 91771-62-9
- IUPAC Name:
- Cyperus scariosus, ext.
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Batch manufactured and supplied by : Jagat Aroma Oils DistIllery
Kannauj-209725
Uttar Pradesh, India
Test item : Caprilo (Cyperus Scariosus ext. Oil)
CAS No. : 91771-62-9/68916-60-9
Physical appearance : Amber coloured or Light Brown Liquid
Purity as per certificate of analysis : 100% Naturally pure
Batch No. : Lot # 11/CYP/2016
Manufactured date : November 2016
Expiry date : October 2018
Recommended storage condition : Ambient (+15 to +25 °C)
Notes: a) Date of receipt of test item at test facility: 05 December 2017
b) Test Item code by test facility: J09-01
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- not specififed
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The medium used for the test was reconstituted water, as described in the OECD test guideline 202 (OECD, 2004). The medium was prepared as per the details given in Appendix 2.
All chemicals used in the study were of either analytical reagent (AR) or better grade reagent.
Chemical Batch No. Manufacturer
Magnesium sulphate. 7H2O H14A/1813/1707/31 s. d. fine-chem Limited
Mumbai-400030
India
E.D.T.A-disodium salt F10Z/1710/1705/31
Methyl orange indicator D16Z/1112/2402/74
Ammonium chloride D14A/2314/2103/31
Patton & Meders indicator F11Z/0311/1102/31
Ammonium hydroxide L11A/0711/2202/31
Calcium chloride, 2H2O D14A/1314/0804/31
Sodium hydroxide D16A/0415/2112/31
Hydroxyl ammonium chloride 1504/17101-1 Qualigens Fine Chemicals
Navi Mumbai – 400 710
India
Potassium chloride QJ3P631025 Merck Specialities Private Limited
Mumbai-400018
India
Sodium carbonate QC2Q620547
Erichrome Black T AL0A601067
Sodium chloride QL4Q642763
Phenolphthalein Indicator 0000245703 Himedia laboratories Pvt. Ltd.
Mumbai – 400086, India
Sodium bicarbonate 0000230345
Hydrochloric acid SHBG4416V Sigma Aldrich, 3050 Spruce street, St. Louis, USA
Test item stock of 1 mg/mL was prepared by mixing 100 mg of test item in test medium and making up the final volume to 100 mL using test medium.
To prepare test concentrations of 0.4 (G2), 0.88 (G3), 1.94 (G4), 4.27 (G5), 9.39 (G6), 20.66 (G7) and 45.46 (G8) mg/L, 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, and 45.46 mL of test item stock was added and volume was made up to 1 L in a volumetric flask using test medium separately.
To prepare test concentrations of 100 mg/L, 100 mg of test item was mixed and made up to 1 L in a volumetric flask using test medium.
1 L of test medium will be used as negative control (G1).
A stock solution of reference substance (Potassium dichromate- K2Cr2O7) of 1 mg/mL was prepared by mixing 10 mg in 10 mL of test medium.
G10 (Reference substance 0.32 mg/L): 0.32 mL of stock of reference substance was mixed and made up to 1 L in a volumetric flask using test medium.
150 mL each of negative control, test item concentrations and reference substance was dispensed separately into 4 test containers.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna was used as test system as it is a recommended species in the guideline.
6.1.2.1 Source
Ecotoxicology
Eurofins Advinus Limited
Bengaluru
India
6.1.2.2 Maintenance of Daphnia
Daphnia were maintained in the test medium. The test medium in which the daphnia were maintained was changed at least twice a week and they were fed with the unicellular green alga, Chlorella vulgaris.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- not specififed
- Test temperature:
- 20 ± 1 oC
- pH:
- ca. 7.8
- Dissolved oxygen:
- not specififed
- Salinity:
- not specififed
- Conductivity:
- not specififed
- Nominal and measured concentrations:
- 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L
- Details on test conditions:
- 6.2.1 Outline of the Method
Neonates of daphnia aged less than 24 hour old were exposed to a series of concentrations of the test item, Caprilo (Cyperus Scariosus ext. Oil) for 48 hours during range finding test and definitive test. Certain concentrations result in certain percentage of daphnia no longer being capable of swimming at 48 hours. The effects on the organisms were assessed at 24 and 48 hours interval and the EC50 value was calculated.
6.2.2 Neonates of Daphnia
One day prior to the start of the treatment, about 100 daphnia during range finding test and definitive test were incubated in approximately 2 L of test medium. The following day, the young ones hatched which were aged less than 24 hour old were collected and used in the test.
6.2.3 Exposure to Treatment
Daphnia were exposed to the treatment under the following conditions during range finding and the definitive test.
Parameters Range finding test Definitive test
Method of exposure Static Static
Duration of the test 48 ± 1 hours 48 ± 1 hours
Test containers 250 mL glass beakers 250 mL glass beakers
No. of daphnia Ten daphnia per group divided into two replicates of five daphnia each Twenty daphnia per group divided into four replicates of five daphnia each
Loading About 15 mL of test medium to each daphnia About 30 mL of test medium to each daphnia
Light Complete darkness Complete darkness
Temperature of growth chamber (C) 20.4 to 20.5 20.3 to 20.5
Dissolved oxygen concentration of test concentration (mg/L) 7.2 to 7.4 7.2 to 7.4
pH of test concentration 7.73 to 7.83 7.73 to 7.83
Aeration The test containers were not bubble aerated The test containers were not bubble aerated
Feeding None None
6.2.4 Test Item Solubility
One gram (100 mg) of the test item was mixed and made up to 10 mL using test medium in a volumetric flask.
6.2.4.1 Range Finding Test
Neonates of daphnia were tested for immobility in test concentrations of Caprilo (Cyperus Scariosus ext. Oil) at 0.0001, 0.001, 0.01, 0.1, 1, 10 and 100 mg/L along with a negative control.
6.2.5 Definitive Test
Based on the results of the range finding test, Caprilo (Cyperus Scariosus ext. Oil) was tested at the concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L (factor of 2.2) were tested in the definitive test. Neonates of daphnia were tested for immobility in these test concentrations along with a negative control and a reference substance.
6.2.5.1 Preparation of Test Concentrations
Test item stock of 1 mg/mL was prepared by mixing 100 mg of test item in test medium and making up the final volume to 100 mL using test medium.
To prepare test concentrations of 0.4 (G2), 0.88 (G3), 1.94 (G4), 4.27 (G5), 9.39 (G6), 20.66 (G7) and 45.46 (G8) mg/L, 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, and 45.46 mL of test item stock was added and volume was made up to 1 L in a volumetric flask using test medium separately.
To prepare test concentrations of 100 mg/L, 100 mg of test item was mixed and made up to 1 L in a volumetric flask using test medium.
1 L of test medium will be used as negative control (G1).
A stock solution of reference substance (Potassium dichromate- K2Cr2O7) of 1 mg/mL was prepared by mixing 10 mg in 10 mL of test medium.
G10 (Reference substance 0.32 mg/L): 0.32 mL of stock of reference substance was mixed and made up to 1 L in a volumetric flask using test medium.
150 mL each of negative control, test item concentrations and reference substance was dispensed separately into 4 test containers.
6.2.6 Observations
Following parameters were observed and recorded during both range finding test and definitive test.
pH and dissolved oxygen concentration of test concentrations at the start and end of the treatment period were recorded.
Immobility of daphnia at 24 ± 1 and 48 ± 1 hour after the start of the treatment was recorded.
The temperature of the incubator/growth chamber was recorded once daily. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 5.655 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value for Caprilo (Cyperus Scariosus ext. Oil) at 48 hours was 5.655 mg/L based on nominal concentrations with fiducial limits at 95 % being 3.8905 and 8.2185 mg/L.
- Executive summary:
1.1.1 Outline of the Method
Neonates of daphnia aged less than 24 hour old were exposed to a series of concentrations of the test item,Caprilo (Cyperus Scariosus ext. Oil)for 48 hours during range finding test and definitive test. Certain concentrations result in certain percentage of daphniano longer being capable of swimming at 48 hours. The effects on the organisms were assessed at 24 and 48 hours interval and the EC50value was calculated.
1.1.2 NeonatesofDaphnia
One day prior to the start of the treatment, about 100 daphnia during range finding test and definitive test were incubated in approximately 2 L of test medium. The following day, the young ones hatched which were aged less than 24 hour old were collected and used in the test.
1.1.3 Exposure to Treatment
Daphnia were exposed to the treatment under the following conditions during range finding and the definitive test.
Parameters
Range finding test
Definitive test
Method of exposure
Static
Static
Duration of the test
48 ± 1 hours
48 ± 1 hours
Test containers
250 mL glass beakers
250 mL glass beakers
No. of daphnia
Ten daphniaper group divided into two replicates of five daphnia each
Twenty daphniaper group divided into four replicates of five daphnia each
Loading
About 15 mL of test medium to each daphnia
About 30 mL of test medium to each daphnia
Light
Complete darkness
Complete darkness
Temperature of growth chamber (°C)
20.4 to 20.5
20.3 to 20.5
Dissolved oxygen concentration of test concentration (mg/L)
7.2 to 7.4
7.2 to 7.4
pH of test concentration
7.73 to 7.83
7.73 to 7.83
Aeration
The test containers were not bubble aerated
The test containers were not bubble aerated
Feeding
None
None
1.1.4 Test Item Solubility
One gram (100 mg) of the test item was mixed and made up to 10 mL using test medium in a volumetric flask.
1.1.4.1 Range Finding Test
Neonates of daphnia were tested for immobility in test concentrations of Caprilo (Cyperus Scariosus ext. Oil) at 0.0001, 0.001, 0.01, 0.1, 1, 10 and 100 mg/L along with a negative control.
1.1.5 Definitive Test
Based on the results of the range finding test, Caprilo (Cyperus Scariosus ext. Oil) was tested at the concentrations of 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, 45.46 and 100 mg/L (factor of 2.2) were tested in the definitive test. Neonates of daphnia were tested for immobility in these test concentrations along with a negative control and a reference substance.
1.1.5.1 Preparation ofTestConcentrations
Test item stock of 1 mg/mL was prepared by mixing 100 mg of test item in test medium and making up the final volume to 100 mL using test medium.
To prepare test concentrations of 0.4 (G2), 0.88 (G3), 1.94 (G4), 4.27 (G5), 9.39 (G6), 20.66 (G7) and 45.46 (G8) mg/L, 0.4, 0.88, 1.94, 4.27, 9.39, 20.66, and 45.46 mL of test item stock was added and volume was made up to 1 L in a volumetric flask using test medium separately.
To prepare test concentrations of 100 mg/L, 100 mg of test item was mixed and made up to 1 L in a volumetric flask using test medium.
1 L of test medium will be used as negative control (G1).
A stock solution of reference substance (Potassium dichromate- K2Cr2O7) of 1 mg/mL was prepared by mixing 10 mg in 10 mL of test medium.
G10 (Reference substance 0.32 mg/L): 0.32 mL of stock of reference substance was mixed and made up to 1 L in a volumetric flask using test medium.
150 mL each of negative control, test item concentrations and reference substance was dispensed separately into 4 test containers.
1.1.6 Observations
Following parameters were observed and recorded during both range finding test and definitive test.
pH and dissolved oxygen concentration of test concentrations at the start and end of the treatment period were recorded.
Immobility of daphnia at 24 ± 1 and 48 ± 1 hour after the start of the treatment was recorded.
The temperature of the incubator/growth chamber was recorded once daily.
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