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EC number: 902-537-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water
- IUPAC Name:
- Reaction mass of tetrahydroxysilane and choline chloride and calcium chloride and water
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Lot 08E20
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- Actisil was dissolved in deionised water to produce solutions that would produce the equivalent of 2 L/ha and 10 L/ha when added to additional deionised water and soil. For both the carbon and the nitrogen transformation test, the moisture content of the soil was adjusted to approximately 40% of the maximum water holding capaity (MWHC) and this achieved via the dosing of each treatment group with the test solution dose, plus additional water as required.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Moisture:
- 35%
- Details on test conditions:
- Soil: LUFA 2.3
sand content: 60.8%
pH water: 7.1
organic carbon content: 0.98%
microbial biomass: 1.9% of total organic carbon
replicates: 3 for both test
Each replicate for both tests was contained in a 2.0 L plastic container (16.7 x 16.7 x 9 cm) and perforated with a single 3 mm diameter hole to enable aerobic conditions to previal throughout the test.
soil amended with ground lucerne (0.5% w:w) for the nitrification assay.
The products of the process of nitrification were extracted from the soil on Day 0, 7, 14 and 28.
Substrate-induceed (glucose) respiration measurements were made on Day 0, 7, 14 and 28 by measuring the carbon dioxide evolution over a 12 hour period. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 other: L/ha
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 10 other: L/ha
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- respiration rate
- Details on results:
- The soil nitrification phase was determined on Day 28 as the difference between the control (water) mean and the treatments was less than 25%. The nitrate level in the 2 L Actisil/ha dry soil test rate was -3.2% from the control mean and the 10 L Actisil/ha dry soil rate was +1.7% at Day 28. The average rate of production of nitrate from Day 0 to Day 28 for the 2 L Actisil/ha test rate was -5.6% different from the control mean and a difference of 3.4% was recorded in the 10 L Actisil/ha treatment group. The percentage variation winthin the control vessels for nitrate concentration (mg/kg dry soil) between replicates ranged from -0.7% to +1.2% on Day 2, thus fulfilling the validity criterion for control treatment replication.
The soil respiration phase was determined on Day 28, according to the guidelines. the difference between the mean control (deionised water) rate of carbon dioxide production (mgCO2/kg/hr) and in any treamtnet group was less than 25% by Day 28. The caron dioxide produced by the 2 L Actisil/ha dry soil basis was +10.8% from the mean of the control group and, similarly, the 10 L Actisil/ha dry soil group was +19.6% on Day 28.
The percentage variation within the control samples (deionised water) for carbon dioxide production (mg Co2/kg/hr) ranged from -5.2% to +5.7% on Day 28. This data fulfils the validity criterion that the variation between control replicates from the average should not be greater than 15%.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At Day 28, average soil carbon respiration rates and nitrate production in soils treated with 2 L/ha and 10 L/ha Actisil dry soil were not greater than 25% from the control means. For this reason it is considered that the test item, Actisil, at the rates shown is unlikely to have any long term effect on the soil microflora which are repsonsible for carbon breakdown and nitrification in the soil.
The study satisfied the OECD 216 (2000) and OECD 217 (2000) validity criterion that the variation between replicate control samples (deionised water) should not be greater than +- 15% at Day 28 when the difference between the controls and the treatments was tested.
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