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EC number: 915-009-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)
- Principles of method if other than guideline:
- In this Zahn-Wellens biodegradation study, the toxicity of the test compound to the activated sludge microorganisms was assessed.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- Samples filtered through 0.45 micron pore-size filters.
- Vehicle:
- not specified
- Details on test solutions:
- Biological testing medium containing calcium, magnesium, and iron cations at 0.25, 0.1, and 0.001 mmoles/L.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The inoculum was either non-adapted activated sludge collected at the municipal water treatment plant in Prague or an activated sludge adapted at different sludge age in the range of 5–30 days, which corresponds to the relations in medium- to low-load biological water treatment plants
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 d
- Nominal and measured concentrations:
- 100 mg/L DTPA (acid form)
- Details on test conditions:
- Two methods were combined for evaluating the complete biodegradability: the method of assaying dissolved organic carbon (EN ISO 7827) suitable for assessing ready biodegradability (X0 1⁄4 30 mg l-1, S01⁄4 DOC 50 mg l-1) and the Zahn-Wellens Test (EN ISO 9888) for assessing potential biodegradability (X0 1⁄4 30 mg l-1, S01⁄4 DOC 50 mg l-1).
- Duration:
- 30 d
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: DOC removal
- Details on results:
- Checks for inhibition and for abiotic processes were performed in parallel with biodegradability tests according to standards (EN ISO 7827 and EN ISO 9888). The results of these checks were negative in all cases. At the concentrations under study, the substances had no inhibitory effects on biological processes and no evidence of abiotic decomposition was obtained.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- DTPA did not biodegrade in municipal activated sludge with sludge ages ranging from 5 to 30 days. DTPA was not inhibitory to biological processes at a concentration of 100 mg/L (acid form).
- Executive summary:
DTPA did not biodegrade in municipal activated sludge with sludge ages ranging from 5 to 30 days. DTPA was not inhibitory to biological processes at a concentration of 100 mg/L (acid form).
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)
- Principles of method if other than guideline:
- In this Zahn-Wellens biodegradation study, the toxicity of the test compound to the activated sludge microorganisms was assessed.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- Samples filtered through 0.45 micron pore-size filters.
- Vehicle:
- not specified
- Details on test solutions:
- Biological testing medium containing calcium, magnesium, and iron cations at 0.25, 0.1, and 0.001 mmoles/L.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The inoculum was either non-adapted activated sludge collected at the municipal water treatment plant in Prague or an activated sludge adapted at different sludge age in the range of 5–30 days, which corresponds to the relations in medium- to low-load biological water treatment plants
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 d
- Nominal and measured concentrations:
- 100 mg/L EDTA (acid form)
- Details on test conditions:
- Two methods were combined for evaluating the complete biodegradability: the method of assaying dissolved organic carbon (EN ISO 7827) suitable for assessing ready biodegradability (X0 1⁄4 30 mg l-1, S01⁄4 DOC 50 mg l-1) and the Zahn-Wellens Test (EN ISO 9888) for assessing potential biodegradability (X0 1⁄4 30 mg l-1, S01⁄4 DOC 50 mg l-1).
- Duration:
- 30 d
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: DOC removal
- Details on results:
- Checks for inhibition and for abiotic processes were performed in parallel with biodegradability tests according to standards (EN ISO 7827 and EN ISO 9888). The results of these checks were negative in all cases. At the concentrations under study, the substances had no inhibitory effects on biological processes and no evidence of abiotic decomposition was obtained.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EDTA did not biodegrade in municipal activated sludge with sludge ages ranging from 5 to 30 days. EDTA was not inhibitory to biological processes at a concentration of 100 mg/L (acid form).
- Executive summary:
EDTA did not biodegrade in municipal activated sludge with sludge ages ranging from 5 to 30 days. EDTA was not inhibitory to biological processes at a concentration of 100 mg/L (acid form).
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)
- Principles of method if other than guideline:
- In this Zahn-Wellens biodegradation study, the toxicity of the test compound to the activated sludge microorganisms was assessed.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- Samples filtered through 0.45 micron pore-size filters.
- Vehicle:
- not specified
- Details on test solutions:
- Biological testing medium containing calcium, magnesium, and iron cations at 0.25, 0.1, and 0.001 mmoles/L.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- The inoculum was either non-adapted activated sludge collected at the municipal water treatment plant in Prague or an activated sludge adapted at different sludge age in the range of 5–30 days, which corresponds to the relations in medium- to low-load biological water treatment plants
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 d
- Nominal and measured concentrations:
- 100 mg/L HEDTA (acid form)
- Details on test conditions:
- Two methods were combined for evaluating the complete biodegradability: the method of assaying dissolved organic carbon (EN ISO 7827) suitable for assessing ready biodegradability (X0 1⁄4 30 mg l-1, S01⁄4 DOC 50 mg l-1) and the Zahn-Wellens Test (EN ISO 9888) for assessing potential biodegradability (X0 1⁄4 30 mg l-1, S01⁄4 DOC 50 mg l-1).
- Duration:
- 30 d
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: DOC removal
- Details on results:
- Checks for inhibition and for abiotic processes were performed in parallel with biodegradability tests according to standards (EN ISO 7827 and EN ISO 9888). The results of these checks were negative in all cases. At the concentrations under study, the substances had no inhibitory effects on biological processes and no evidence of abiotic decomposition was obtained.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- HEDTA did not biodegrade in municipal activated sludge with sludge ages ranging from 5 to 30 days. HEDTA was not inhibitory to biological processes at a concentration of 100 mg/L (acid form).
- Executive summary:
HEDTA did not biodegrade in municipal activated sludge with sludge ages ranging from 5 to 30 days. HEDTA was not inhibitory to biological processes at a concentration of 100 mg/L (acid form).
Referenceopen allclose all
Description of key information
The toxicity to microorganisms was not determined as emission to STP is unlikely.
Also, toxicity to microorganisms is unlikely because none of the chelating agents are toxic to microorganisms (see provided studies) and these chelating agents will capture the metal ions, reducing any toxicity of the captured metal ions.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.