(S)-3-methyl-2-(2-oxotetrahydropyrimidine-1-yl)butyric acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 6-8 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

430-900-2

EC Name:

-

Cas Number:

192725-50-1

Molecular formula:

Hill formula: C9H16N2O3 CAS formula: C9H16N2O3

IUPAC Name:

3-methyl-2-(2-oxo-1,3-diazinan-1-yl)butanoic acid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At 0 and 48 hours. samples were removed from the treatment vessels and anlyzed by the HPLC method. the exposure concentration was achieved.

Test solutions

Vehicle:

no

Details on test solutions:

The test solution was prepared by transferring weighted amounts of A-164220.0 into the glas beakers. The containers were made up to 200 mL with reconsituted water in order to obtain the final concentration of 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Fill in what you can

TEST ORGANISM
- Common name: Crustacean species
- Source: laboratory breeding

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Oberservation of immobilization was made at 0 and 48 hours. Daphnia was considered immobile if not able to swim within 15 seconds of gentle agitation of the test container.

Test conditions

Hardness:

251 mg/L

Test temperature:

monitored during the study

pH:

monitored during the study

Nominal and measured concentrations:

Nominal conc = 100 mg/l
Measured conc = 108.31 mg/l @ 0 hours
Measured conc = 108.18 mg/l @ 48 hours

Details on test conditions:

The values of dissolved oxygen, pH and temperature alwaysreminaed within the acceptable limits.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of A-164220.0 at 24 and 48 hours were higher tha 100mg/L