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EC number: 258-380-0 | CAS number: 53126-67-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- from 2007-02-22 to 2007-05-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- The study was conducted in accordance with ISO/IEC 17025.
- Specific details on test material used for the study:
- Batch number: CHA0029466
Active ingredient (a.i.) content, nominal: 100% - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Prior to the test, a batch of reconstituted water was aerated vigorously over a period of 2 h. Final test concentrations were prepared with this aerated water by dilution of stock solutions of the test substance. 2 h before the test, vessels were filled with the formulated media to allow equilibration of pH, oxygen and temperature.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Straus, 1820
- Source: in-house culture
- Age of parental stock: Parental and young Daphnia
- Feeding during test: no feeding
ACCLIMATION
- Acclimation period: Daphnia, which had been selected in size, were acclimatized to the reconstituted water for 4 hours before introduction into the test media.
- Acclimation conditions: same as test
- Type and amount of food: 15 g sera micropan (sera GmbH, D-52525 Heinsberg) homogenized in 1 L deionised water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 ± 0.5 °C
- pH:
- 7.2 - 7.7
- Dissolved oxygen:
- 6.3 - 6.4 mg/L
- Salinity:
- NA
- Conductivity:
- <1.5 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 1, 10 and 100 mg/L
No measured concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Material, size, headspace, fill volume: 100 mL flasks, all-glass, with 50 mL of test medium
- Aeration: no aeration
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (lSO 6341) prepared with distilled water (conductivity <1.5 µS/cm). The final test medium contained 25 mL/L of each of the following four stock solutions: CaCl2 x 2H2O, 11.76 g/L; MgSO4 x7H2O,4.93 g/L; NaHCO3, 2.59 g/L; KCl, 0.23 g/L. The sum of the Ca and Mg ions in this solution is 2.5 mmol/L.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h photoperiod a day
- Light intensity: overhead white fluorescent tubes - Reference substance (positive control):
- yes
- Remarks:
- Periodically conducted reference test with potassium dichromate.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- At all nominal test concentrations no significant effects (<10 % immobilization) were observed after 24 and 48 h of exposure. No significant effects were also observed in the control atler 24 and 48 h of exposure.
- Results with reference substance (positive control):
- Not reported.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No significant effects were observed in the control and the test concentrations. Therefore, the EC50 was determined to be >100 mg/L.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and ISO 6341. The study was conducted in a static system over a period of 48 hours with test concentration of 1, 10 and 100 mg/L. 40 daphnids per test concentration in two replicates and control in three replicates were tested. The test item concentration was not determined, thus, all biological endpoints were based on the nominal concentration. There was no immobilisation observed in twenty daphnids exposed to each test item treated and control group. Further no abnormal behaviour or appearance of test animals was noted. The estimated EC50 value (48 hours) of the test item based on the nominal concentration is >100 mg/L.
Reference
Table 1: lmmobilisation of Daphnia magna after 24 and 48 h of exposure to the test material
Nominal concentration (mg/L) |
Code |
Number of individuals per test vessel |
Immobile Daphnia (No./vessel) 24 h |
Immobile Daphnia (No./vessel) 48 h |
% immobile Daphnia after 24 h of exposure |
% immobile Daphnia after 48 h of exposure |
Control 0 |
A B C |
20 20 20 |
0 0 0 |
0 0 0 |
0 0 0 |
0 0 0 |
1 |
A B |
20 20 |
0 0 |
0 0 |
0 0 |
0 0 |
10 |
A B |
20 20 |
0 0 |
0 1 |
0 0 |
0 5 |
100 |
A B |
20 20 |
0 0 |
0 0 |
0 0 |
0 0 |
Description of key information
No significant effects were observed in the control and the test concentrations for both the target and the source substances. Therefore, the EC50 was determined to be >100 mg/L.
Key value for chemical safety assessment
Additional information
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and ISO 6341. The study was conducted in a static system over a period of 48 hours with test concentration of 1, 10 and 100 mg/L. 40 daphnids per test concentration in two replicates and control in three replicates were tested. The test item concentration was not determined, thus, all biological endpoints were based on the nominal concentration. There was no immobilisation observed in twenty daphnids exposed to each test item treated and control group. Further no abnormal behaviour or appearance of test animals was noted. The estimated EC50 value (48 hours) of the test item based on the nominal concentration is >100 mg/L.
Additionally, a read-across to Dibutyl hydrogen phosphate (CAS 107-66-4) was performed in a weight of evidence approach. A study was performed according to OECD 202 and Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) to assess the acute toxicity of the source item to Daphnia magna STRAUS under semi-static conditions. A range finding test preceded the main test. The following nominal concentrations of the source item were tested in the range finding test: 1, 3.2, 10, 32 and 100 mg/L. In the main test, Daphnia were exposed to the source item added to dilution water at a limit test concentration of nominally 100 mg/L for a period of 48 hours. The main test was conducted as a semi-static test with daily renewal of test medium. During the test a temperature range of 18 - 22 °C was maintained in the test vessels, with a maximum temperature fluctuation of ± 1 °C in each individual test. The temperature, the pH and the oxygen values were measured every day in the freshly prepared media and in the media after 24 hours of exposure. Immobilisation rates and any abnormal behaviour were recorded at 24-hour intervals. Analytically determined values of the test concentration varied not more than 6.2 %, therefore, the EC50 is given as nominal concentration. At the limit concentration no toxic effects against Daphnia were observed at the end of the 48 hour study period. After 48 hours of exposure an EC50 of >100 mg/L (nominal) for the source substance was determined.
Both the target and the source substance showed no effects on Daphnia magna after 48 hour exposure at a limit test concentration of 100 mg/L. The EC50 for both substances was determined to be >100 mg/L. In conclusion, the target substance is not significantly toxic to Daphnia magna up to a concentration of 100 mg/L.
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