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EC number: 701-473-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - August 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: Collected from the aeration tank of a sewage treatment plant unit treating predominantly domestic sewage at Siddaganga Institute of Technology, Chandana complex, Tumkur – 572103, India
- Pretreatment: A well-mixed, measured volume of an activated sludge was filtered through a pre-weighed crucible. The crucible was heated to constant mass at 105ºC for 3 hours 14 minutes and then weighed.
- Preparation: Accurately 23.6 mL of the inoculum was added to each of the flasks to attain a concentration of 30 mg/L of suspended solids. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The dilution water was prepared by adding 1 mL of each of four salt solutions to 500 mL of deionised water. This solution was diluted to 1000 mL and mixed well.
- Additional substrate: No
- pH adjusted: No
- Aeration of dilution water: Aeration was applied for 1 hour at 20 ± 2°C.
- Continuous darkness: Yes
- Temperature: 22 ± 2°C
TEST SYSTEM
- Culturing apparatus: Incubator
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Aeration of dilution water for 1 hour
SAMPLING
- Sampling frequency: After 0, 3, 6, 9, 14, 19, 25 and 28 d
- Sample storage before analysis: No
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles
- Toxicity control: 2 bottles - Reference substance:
- acetic acid, sodium salt
- Test performance:
- The test item reached a maximum mean biodegradation value of 80.26% (% of ThCO2) for two bottles within 28 days exceeding the criterium of 60% biodegradation given by the guideline. The differences in replicate values of the removal of the test substance at the end of the test were less than 20% (i.e.1.49%).
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 80.26
- Sampling time:
- 28 d
- Details on results:
- The total CO2 evolution in the control blanks at the end of the test was 1.98 mg/L which is less than 40 mg/L of validity criteria. The CO2 absorber traps were titrated on 29th day after acidifying the test flasks on 28th day. This acidification drives off any dissolved CO2 present in the test vessels. The results of the 0.05N HCl consumed for all the test flasks from the absorber vessels on Day 29 showed no significant change in all test flasks compared with the blank BaOH2 and confirmed that no significant production of CO2 into the absorber vessels occurred after acidifying the test flasks. All the criteria for acceptability of the test were met. Hence, the present study was considered to be valid.
- Results with reference substance:
- The percentage degradation of the procedure control substance (Sodium acetate) attained 62.91% (% of ThCO2) biodegradation by 14 days (greater than 60% degradation).
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The results of the CO2 Evolution Test indicated that the test item is readily biodegradable by OECD criteria. The test item was readily biodegradable within a 10-days window and after 28 days (80.26% of ThCO2).
- Executive summary:
To assess the ready biodegradability of the test item a GLP-compliant CO2 Evolution Test (Modified Sturm Test) according to OECD TG No. 301 B (adopted 17th July 1992) was carried out. During the study, the amount (mg) of CO2 produced was calculated at the start of the experiment on day 0, 3, 6, 9, 14, 19, 25, 28 and 29 respectively. The relative biodegradation values were calculated from the CO2 measurements during the test period of 28 days. The test item reached a maximum mean biodegradation value of 80.26 (% of ThCO2) for two bottles within 28 days which exceeded the criteria of 60% biodegradation. The differences in replicate values of the removal of the test substance at the end of the test were less than 20% (i.e. 1.49%). The percentage degradation of the procedure control substance (Sodium acetate) attained 62.91% (% of ThCO2) biodegradation after 14 days (greater than 60% degradation). The total CO2 evolution in the control blanks at the end of the test was 1.98 mg/L which is less than 40 mg/L of validity criteria. The CO2 absorber traps were titrated on 29th day after acidifying the test flasks on 28th day. This acidification drives off any dissolved CO2 present in the test vessels. The results of the 0.05N HCl consumed for all the test flasks from the absorber vessels on Day 29 showed no significant change in all test flasks compared with the blank BaOH2 and confirmed that no significant production of CO2 into the absorber vessels occurred after acidifying the test flasks. All the criteria for acceptability of the test were met. Hence, the present study was considered to be valid. The results of the CO2 Evolution Test indicated that the test item is readily biodegradable by OECD criteria. The test item was readily biodegradable within a 10-days window and after 28 days (80.26% of ThCO2) under the conditions of the CO2 Evolution test.
Reference
TABLE 1: PERCENTAGE OF DEGRADATION (% ThCO2)
Flask Content |
Flask No. |
Conc. of TOC in mg per litre |
Percentage of Degradation (% ThCO2) |
||||||
Day 3 |
Day 6 |
Day 9 |
Day 14 |
Day 19 |
Day 25 |
Day 28 |
|||
Reference substance- Sodium acetate |
3 |
15
|
11.32 |
18.72 |
40.20 |
63.41 |
74.50 |
84.39 |
92.98 |
4 |
11.12 |
18.12 |
39.40 |
62.41 |
74.17 |
83.59 |
91.92 |
||
Mean |
11.22 |
18.42 |
39.80 |
62.91 |
74.33 |
83.99 |
92.45 |
||
Difference Between the Replicates 3 and 4 |
-1.07 |
||||||||
|
Percentage Difference |
-1.15 |
|||||||
Test Item- LYAF |
5 |
15
|
3.00 |
5.59 |
21.61 |
39.63 |
54.45 |
66.27 |
79.66 |
6 |
3.66 |
6.79 |
22.75 |
40.63 |
55.18 |
67.34 |
80.86 |
||
Mean |
3.33 |
6.19 |
22.18 |
40.13 |
54.82 |
66.81 |
80.26 |
||
Difference Between the Replicates 5 and 6 |
1.20 |
||||||||
|
Percentage Difference |
1.49 |
Description of key information
The results of the CO2 Evolution Test indicated that the test item is readily biodegradable by OECD criteria. The test item was readily biodegradable within a 10-days window and after 28 days (80.26% of ThCO2).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
To assess the ready biodegradability of the test item a GLP-compliant CO2 Evolution Test (Modified Sturm Test) according to OECD TG No. 301 B (adopted 17th July 1992) was carried out. During the study, the amount (mg) of CO2 produced was calculated at the start of the experiment on day 0, 3, 6, 9, 14, 19, 25, 28 and 29 respectively. The relative biodegradation values were calculated from the CO2measurements during the test period of 28 days. The test item reached a maximum mean biodegradation value of 80.26 (% of ThCO2) for two bottles within 28 days which exceeded the criteria of 60% biodegradation. The differences in replicate values of the removal of the test substance at the end of the test were less than 20% (i.e. 1.49%). The percentage degradation of the procedure control substance (Sodium acetate) attained 62.91% (% of ThCO2) biodegradation after 14 days (greater than 60% degradation). The total CO2 evolution in the control blanks at the end of the test was 1.98 mg/L which is less than 40 mg/L of validity criteria. The CO2 absorber traps were titrated on 29th day after acidifying the test flasks on 28th day. This acidification drives off any dissolved CO2 present in the test vessels. The results of the 0.05N HCl consumed for all the test flasks from the absorber vessels on Day 29 showed no significant change in all test flasks compared with the blank BaOH2 and confirmed that no significant production of CO2 into the absorber vessels occurred after acidifying the test flasks. All the criteria for acceptability of the test were met. Hence, the present study was considered to be valid. The results of the CO2 Evolution Test indicated that the test item is readily biodegradable by OECD criteria. The test item was readily biodegradable within a 10-days window and after 28 days (80.26% of ThCO2) under the conditions of the CO2 Evolution test.
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