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EC number: 614-598-1 | CAS number: 68553-12-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6 May to 6 November 1998 (experimental dates)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- draft
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: 813771
- Expiration date of the lot/batch: No data
- Purity test date: No data
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, over silica gel in the dark
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: No data
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No data
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and nominal 2.0 mg/L
- Sampling method: Water samples taken from control and each replicate test vessel at 0h (fresh), 24, 48 and 72 h (old and fresh), 96h (old)
- Sample storage conditions before analysis: Duplicate samples taken and frozen (~-20oC) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test solutions prepared by direct dispersion in water. Test material was dispersed in dechlorinated tap water with high shear mixing (7800 rpm for 5 mins), then volume adjusted to 2 litres. Stock dispersion further dispersed to 22 litres with propeller stirring for approximately 24h
- Eluate: Dechlorinated tap water
- Differential loading: No
- Controls: Dechlorinated tap water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None recorded by visual inspection - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: No data
- Source: Parkwood Trout Farm, Harrietsham, Kent , UK
- Age at study initiation (mean and range, SD): No data
- Length at study end of definitive study (length definition, mean and SD): 4.6 cm (SD = 0.2 cm)
- Weight at study end of definitive study (mean and SD): 1.13 g (SD = 0.17 g)
- Method of breeding: Not applicable
- Maintenance of the brood fish: Not applicable
ACCLIMATION
- Acclimation period: 21 October to 02 November 1998
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food during acclimation: Commerical trout pellets
- Feeding frequency during acclimation: Discontinued 48 h prior to study
- Health during acclimation (any mortality observed): 1% mortality in 7 days prior to test
FEEDING DURING TEST
- Food type: Not fed during test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- No data
- Test temperature:
- 13 +/-1oC
- pH:
- 7.2 - 7.9
- Dissolved oxygen:
- 8.4 - 9.8
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal concentration: Control and 2.0 mg/L
Measured concentration: 1.4 mg/L (dispersed with test system),- Details on test conditions:
- TEST SYSTEM
- Test vessel: Covered to reduce evaporation
- Material, size, headspace, fill volume: Glass test vessels , 22 L fill volume
- Aeration: Not aerated
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate): Not applicable
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): Not applicable
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8h Light:dark with 20 min transition period
- Light intensity: No data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test
- Range finding study
- Test concentrations: 0.2, 1.0 & 2.0 mg/L (nominal)
- Results used to determine the conditions for the definitive study: No mortality or symptoms observed, therefore limit test at 2.0 mg/L (nominal) for definitive study- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: <0.22 mg/L bioavailable
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: <0.22 mg/L bioavailable
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: <0.22 mg/L bioavailable
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: <0.22 mg/L bioavailable
- Sublethal observations / clinical signs:
Nominal concn
(mg/L)
Total no. organisms tested
Total no. immobilised
Observations
% effect
24h
48h
24h
48h
Control
10
0
0
0
0
0
Control
10
0
0
0
0
0
2.0
10
0
0
0
0
0
2.0
10
0
0
0
0
0
2.0
10
0
0
0
0
0
No sub-lethal effects observed
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to rainbow trout, based on mortality, gave a 96 hour LC50 of >2.0 mg/L (based on nominal test concentrations) and a NOEC of 2.0 mg/L, although the test substance is not toxic to aquatic organisms at the limit of solubility, which is was less than the LoQ.
- Executive summary:
In a 96-h acute toxicity study rainbow trout, Oncorhynchus mykiss, were exposed to a single test concentration under semi-static conditions in accordance with the OECD test guideline 203 and in compliance with GLP. The nominal test concentrations were a control and a 2.0 mg/L test solution prepared. However, the amount of bioavailable (dissolved) test material was shown to be <LoQ (0.22 mg/L) by analysis of filtered test samples during preliminary work. The 96 hour LC50, based on nominal test concentrations was >2.0 mg/L. and the NOEC was 2.0 mg/L, although the test substance is not toxic to aquatic organisms at the limit of solubility, which is was less than the LoQ.
Reference
Description of key information
In a 96-h acute toxicity study rainbow trout, Oncorhynchus mykiss, were exposed to a single test concentration under semi-static conditions in accordance with the OECD test guideline 203 and in compliance with GLP. The nominal test concentrations were a control and a 2.0 mg/L test solution prepared. The time-weighted mean measured concentration was 1.4 mg/L. However, the amount of bioavailable (dissolved) test material was shown to be <LoQ (0.22 mg/L) by analysis of filtered test samples during preliminary work. The 96 hour LC50, based on nominal test concentrations was >2.0 mg/L. and the NOEC was 2.0 mg/L, although the test substance is not toxic to aquatic organisms at the limit of solubility, which is was less than the LoQ.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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