Fatty acids, coco, iso-Bu esters

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

20- 22 Jan 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

GLP - Guideline study, tested with the source substance isopropyl palmitate (CAS 142-91-6). According to the ECHA guidance document “Practical guide 6: How to report read-across and categories (March 2010)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl palmitate

EC Number:

205-571-1

EC Name:

Isopropyl palmitate

Cas Number:

142-91-6

Molecular formula:

C19H38O2

IUPAC Name:

isopropyl palmitate

Details on test material:

- Name of test material (as cited in study report): Isopropylpalmitate
- Physical state: clear liquid
- Analytical purity: 94.3%
- Impurities (identity and concentrations): C-chain distribution
- Lot/batch No.: 27043
- Expiration date of the lot/batch: February 1999

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 1000 and 3000 mg/L
- Sampling method: at 0, 24 and 48 hours

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1000 and 3000 mg were weighed into medium and stirred for 24 hours, after two hours of rest an oily film was developed, the clear part of this solution was carefully removed and used for testing

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: BioInternational B.V.,NJ Horn, The Netherlands
- Age at study initiation: 6 - 24 hours
- Feeding during test: none

ACCLIMATION
- Acclimation conditions: M4 medium, 20°C, 900 Lux, 16/8 hours light/darkness regime
- Type and amount of food: Spirulina
- Feeding frequency: daily, last feed 4.5 hours prior to testing

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19.5 - 20.1 °C

pH:

8.0 - 8.1

Dissolved oxygen:

99 - 101% satuaration

Nominal and measured concentrations:

Nominal: control, 1000 and 3000 mg/L
Measured after 0 hours: control, 0.72 and 1.68 mg/L
Measured after 24 hours: control, 0.30 and 2.35 mg/L
Measured after 48 hours: control, 0.08 and 0.41 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL beakers
- Type (delete if not applicable): closed with watch glasses
- Material, size, headspace, fill volume: 50 mL test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light/darkness
- Light intensity: 900 Lux

EFFECT PARAMETERS MEASURED: immobility after 24 and 48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 3 000 mg/L

Nominal / measured:

nominal

Conc. based on:

other: separated water phase

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50/LC50: EC50(24h)=1.5 mg/L

Any other information on results incl. tables

No immobilisation occurred at any concentration (0%), however daphnids swam at the surface in concentrations 1000 and 3000 mg/L after 24 and 48 hours. Only the dissolved material was applied to avoid physical influence of the test substance. Analytical retrieval of solved substance were below 0.1%.