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Diss Factsheets
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EC number: 813-880-3 | CAS number: 2055396-18-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Some information in this page has been claimed confidential.
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test performed under GLP, according to guidelines, no CoA available.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): glycine N-N bis [2-[bis(carboxymethyl) amine] ethyl] - pentapotasium salt
- Physical state: white powder
- Analytical purity: 97.0%
- Impurities (identity and concentrations): water
- Lot/batch No.: ???
- Storage condition of test material: In original container at room temperature and protected from strong light and moisture
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from the aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage
- Preparation of inoculum for exposure: the sample was allowed to settle and the supernatant filtered through Whatman GFA filter paper (first 250 ml discarded) - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The nutrient medium of the closed bottle test contained per litre of deionized water: 8.5 mg KH2PO4, 21.75 mg K2HPO4, 33.4 mg Na2HPO4.2H2O, 1.7 mg/l NH4Cl, 22.5 mg MgSO4.7H2O, 27.5 mg CaCl2, 0.25 mg FeCl3.6H2O.
- Test temperature: 23 +/- 1 °C
- pH adjusted: no
- Aeration of mineral medium: yes
- Inoculum concentration: 1 drop of inoculum per litre
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: water bath
- Number of culture flasks/concentration:
8 bottles containing only mineral medium (without inoculum, without test or reference substance)
8 bottles containing only inoculum = inoculum blank
8 bottles containing test substance and inoculum =test
8 bottles containing sodium benzoate and inoculum = reference control
- Method used to create aerobic conditions: aeration by agitation
- Measuring equipment: oxygen meter
SAMPLING
- Sampling frequency: day 0, 5, 21 ,28
- Sampling method: the bottles were discarded after the oxygen measurement.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: no
- Reference control: yes
CALCULATIONS:
THOD = 1.0 mg/mg
THOD sodium benzoate = 1.67 mg/mg
Oxygen consumption (mg/l) (BOD) = mean oxygen concentration (mg/l) inoculum blank - mean oxygen concentration (mg/l) test (or reference)
Biodegradation (%) = BOD/THOD *100
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Of the reference substance 67% biodegraded at day 5 and 89% at day 28
Any other information on results incl. tables
Mean oxygen depletion and percentages biodegradation values
O2 depleation (mg/l) | Degradation (%) | |||||
day 5 | day 21 | day 28 | day 5 | day 21 | day 28 | |
Dilution water without inoculum | 0.25 | 0.3 | 0.35 | - | - | - |
Dilution water with inoculum | 0.35 | 0.325 | 0.550 | - | - | - |
Test substance | 0.5 | 0.125 | -0.025 | 10 | 2 | 0 |
Standard substance | 3.325 | 4.75 | 4.425 | 67 | 95 | 89 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- endogenous respiration of <0.6 mg/l; biodegradation of reference substance; >0.5 mg/l of oxygen in bottles during the test and <20% differences between replicates at day 28.
- Conclusions:
- Glycine N-N bis[2-[bis (carboxymethyl) amine] ethyl] -pentapotasium was biodegraded 0% at day 28 in the closed bottle test and can therefore not be classified as readily biodegradable. No biodegradation in this test does not necessarily mean that the test substance is not biodegradable under environmental conditions, but that more work will be necessary to establish this.
- Executive summary:
Glycine N-N bis [2- [bis (carboxymethyl) amine] ethyl]-pentapotassium salt attained insignificant degradation within 28 days and, cannot, therefore be considered as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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