Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Some information in this page has been claimed confidential.
Administrative data
- Endpoint:
- hydrolysis
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is readily biodegradable
Cross-reference
- Reason / purpose for cross-reference:
- data waiving: supporting information
Reference
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- Temperature ranged from 17.7-24.2 degrees C. The pH of the sodium acetate control was not measured after 14 days. The amount of K2HPO4 in the nutrient solution was 21.7g. None of these deviations are thought to have influenced the results.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsubishi-Kagaku Foods Corporation, Batch # 2828911A
- Expiration date of the lot/batch: Aug. 27, 2004
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Solubility and stability of the test substance in the solvent/vehicle: >= 10 mg/L
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final preparation of a solid: The test substance was dissolved in 1000 mL of demineralized water to make a stock solution. From this solution, final solutions of 2.5 and 5.0 mg ThOD/L were made.
FORM AS APPLIED IN THE TEST (if different from that of starting material): Solution - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Oxidation ditch in Hazerswoude, the Netherlands
- Storage conditions: Aerated until use.
- Storage length: 5 days
- Concentration of sludge: 5 mL/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 1.38 mg/L
- Based on:
- test mat.
- Initial conc.:
- 2.77 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: potassium dihydrogen phosphate 8.5 mg/L, dipotassium hydrogen phosphate 21.7 mg/L, disodium monohydrogen phosphate heptahydrate 33.4 mg/L, ammonium chloride 0.5 mg/L, magnesium sulphate heptahydrate 22.5 mg/L, calcium chloride dihydrate 36.4 mg/L, iron (III) chloride hexahydrate 0.25 mg/L
- Test temperature: 17.7-24.2 degrees C
- pH: 6.2-7.3
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 293 ml BOD bottles
- Number of culture flasks/concentration: 16
- Method used to create aerobic conditions: aeration of mineral medium
- Measuring equipment: oxygen electrode
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
- Sampling frequency: Days 0, 7, 14, 21, and 28
- Sampling method: oxygen electrode
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
STATISTICAL METHODS: Average oxygen consumption and standard deviations were calculated. - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69
- Sampling time:
- 21 d
- Remarks on result:
- other:
- Remarks:
- 1.38 mg/L test substance
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 64
- Sampling time:
- 21 d
- Remarks on result:
- other:
- Remarks:
- 2.77 mg/L test substance
- Details on results:
- Inoculum blanks showed no more than 1.50 mg O2/L degradation. The inoculum activity control showed complete biodegradation. The toxicity control showed 38-70% degradation, and thus was vallid.
- Key result
- Parameter:
- BOD5
- Value:
- 1.08 g O2/g test mat.
- Remarks on result:
- other:
- Remarks:
- 1.38 mg/L test concentration
- Key result
- Parameter:
- BOD5
- Value:
- 1.04 g O2/g test mat.
- Remarks on result:
- other:
- Remarks:
- 2.77 mg/L test concentration
- Results with reference substance:
- The reference substance results were valid with over 60% degradation in 10 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance was 64-69% biodegraded in 28 days and met the 10-day window requirement and thus is readily biodegradable.
- Executive summary:
The test substance LMD was tested for biodegradability in a 28-day closed bottle test using concentrations of 1.38 and 2.77 mg/L. The results of the toxicity controls, reference substance, inoculum controls, and incolum activity controls were valid. The test substance was 64-69% degraded in 28 days and met the 10-day window requirement. The test substance is therefore readily biodegradable.
Percent Biodegradation Based on the ThOD/COD (Average)
Initial Concentration Test Substance |
Day 0 |
Day 7 |
Day 14 |
Day 21 |
Day 28 |
1.38 mg/L |
0 |
63 |
67 |
69 |
60 |
2.77 mg/L |
0 |
54 |
63 |
64 |
57 |
Sodium acetate 4.0 mg/L |
0 |
69 |
78 |
n.d. |
n.d. |
2.77 mg/L and 4.0 mg/L sodium acetate |
0 |
61 |
70 |
n.d. |
n.d. |
n.d. – not determined
Data source
Materials and methods
Results and discussion
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.