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EC number: 256-370-0 | CAS number: 49556-16-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28.03.2011 to 31.03.2011
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Hexanoic acid, 3,5,5-trimethyl-, tin (2+)salt (2:1)
- EC Number:
- 700-567-0
- Cas Number:
- 1231728-34-9
- Molecular formula:
- C18H34O4Sn
- IUPAC Name:
- Hexanoic acid, 3,5,5-trimethyl-, tin (2+)salt (2:1)
- Test material form:
- liquid: viscous
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The M4 medium (control) and the test substance preparation (100 mg/L M4 medium) were filtered through membrane filter. 10 mL (5 mL for TIC analysis) filtrate of the test substance preparation and the control were filled into the measuring vessel and firmly closed with aluminium foil. Then, they were placed in the sampling unit and measured automatically as triplicate determination with the TC and TIC analyser.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was found to be poorly water soluble. Therefore, 100 mg test substance were added to 1000 mL M4 medium and stirred for 24 h. The test substance preparation was then filtered through a filter paper. The control (M4 medium) was treated in the same way.
- Controls: M4 medium served as control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): After filtration, the test substance preparation was present as a clear solution
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea (Daphnia magna)
- Strain: Straus
- Source: the organisms were held in the testing laboratory and descended from a population received from 'Landesumweltamt (NRW)', Düsseldorf (Germany) on 30.05.2006
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: The daphnids were held in 2 L beakers filled with ca. 1400 mL M4 medium. 15-20 adult organisms were held in one beaker. The breeding beakers were kept at an artificial light-dark cycle of 16 to 8 h. The breeding beakers were placed in a temperature controlled laboratory at 20 +/- 2°C. The breeding medium was renewed three times a week (Monday, Wednesday, and Friday).
- Feeding during test: no
ACCLIMATION
- Acclimation period: not necessary
- Acclimation conditions (same as test or not): holding/breeding conditions the same as in the test
- Type and amount of food: during holding/breeding the daphnids were fed with Desmodesmus subspicatus from Monday to Friday
- Feeding frequency: daily
- Health during acclimation (any mortality observed): yes
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- at test start: 21.3°C (control), 21.3°C (test substance solution)
after 24 h: 21.1°C (control), 21.1°C (test substance solution)
at test termination: 21.1°C (control), 21.1°C (test substance solution) - pH:
- at test start: 8.09 (control), 7.79 (test substance solution)
after 24 h: 8.20 (control), 7.75 (test substance solution)
at test termination: 8.33 (control), 8.01 (test substance solution) - Dissolved oxygen:
- at test start: 6.18 mg/L (control), 5.88 mg/L (test substance solution)
after 24 h: 5.93 mg/L (control), 5.98 mg/L (test substance solution)
at test termination: 6.09 mg/L (control), 6.04 mg/L (test substance solution) - Nominal and measured concentrations:
- 100 mg/L nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass/50 mL/-/30 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5 (one additional beaker was used for measurement of temperature, oxygen saturation, and pH value)
- No. of vessels per control (replicates): 5 (one additional beaker was used for measurement of temperature, oxygen saturation, and pH value)
- No. of vessels per vehicle control (replicates): no vehicle used
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, oxygen saturation, and pH value were measured daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
- Light intensity: 700 +/- 50 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation (daily)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: only one concentration tested (100 mg/L)
- Justification for using less concentrations than requested by guideline: limit test
- Range finding study: not reported - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality:
- EC50/LC50: 24 h EC50=1.0 mg/L (range given in the guideline: 0.6-2.1 mg/L)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance preparation of nominal 100 mg/L induced no immobilisation to the exposed Daphnia within 48 h of exposure (48 h NOEC >=100 mg/L; 48 h EC50>100 mg/L).
- Executive summary:
The toxicity of the analogue substance hexanoic acid, 3,5,5-trimethyl-, tin (2+)salt (2:1) was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) and EU Method C.2 (Acute Toxicity for Daphnia). In this limit test, daphnids were exposed to a nominal test substance concentration of 100 mg/L over a period of 48 h under static conditions. As the test substance was poorly water soluble a water accommodated fraction was prepared (dissolving of test substance in M4 medium, stirring for 24 h, filtration). DOC analysis showed that no loss of substance occurred during the exposure period. At the concentration tested no immobilisation of daphnids was observed (48 h NOEC>=100 mg/L; 48 h EC50>100 mg/L).
The study is regared as reliable with restrictions (only one concentration was tested) and satiesfies the requirements of the guideline.
Synopsis
Test organism/age: Daphnia magna Straus/<24 h
Test type: static
48 h NOEC: >=100 mg/L
48 h EC50>100 mg/L (values refer to nominal concentrations)
Endpoint(s) affected: mobility
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