Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 816-285-7 | CAS number: 1263133-33-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 002
- Cas Number:
- 1263133-33-0
- Test material form:
- solid
- Details on test material:
- Purity: 97.04%
Impurities: Not reported
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance was directly weighed into each test flask and pure water was added.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Supplied by municipal sewage treatment plant (Bensheim, Germany)
- Conditioning: The activated sludge used was used as collected, but coarse particles were removed by settling for a short period (15 minutes) and then the upper layer decanted. During holding prior to use the sludge was fed daily with 50 mL synthetic sewage per litre and kept aerated at room temperature overnight. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight determined. Based on the sludge dry matter, calculated amounts of wet sludge were suspended in pure water to yield a concentration equivalent to 3 g/L on dry weight basis. This level gave a concentration of 1.5 g/L suspended solids in the test medium. The pH of the activated sludge was 7.2 and therefore no adjustment was necessary.
Study design
- Test type:
- not specified
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2°C
- pH:
- 6.3 to 8.0
- Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks of approximately 1 litre volume and Karlsruher flasks of 250 mL volume.
- Aeration: With compressed air (1.017 litre per minute)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control: Six controls (pure water, synthetic sewage and inoculum, but without addition of the test item) were tested in parallel.
- Sludge concentration (weight of dry solids per volume): 1.5 g/L suspended solids in the test medium.
- Nitrification inhibitor used: N-allylthiourea
OTHER TEST CONDITIONS
- Adjustment of pH: The pH of this solution was adjusted to 7.0 with NaOH.
EFFECT PARAMETERS MEASURED: Respiration rate (oxygen consumption) was measured for 10 minutes after a 3 hour exposure period. Shorter evaluation periods were also possible and done over the most linear part of the measurement. Results were expressed as percent inhibition relative to the control. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Total respiration:
In comparison to the inoculum controls the respiration rates of the activated sludge up to concentrations of 320 mg/L were not inhibited. The highest test concentrations of 1000 mg/L showed a small inhibition; the inhibition was 1.6%, -13.9%, -8.7%, -2.4% and 23.0% for test item concentrations of 10, 32, 100, 320 and 1000 mg/L, respectively.
Respiration without nitrification (Heterotrophic Respiration):
The inhibition of the heterotrophic respiration of the activated sludge was only moderately and not dose-related inhibited up to concentrations of 1000 mg/L; the inhibition was 25.2%, 26.7%, 21.9%, 29.5% and 32.8% for test item concentrations of 10, 32, 100, 320 and 1000 mg/L, respectively.
Respiration based on nitrification:
In comparison to the inoculum controls, the respiration rates of the activated sludge based on nitrification was not inhibited for all test item concentrations up to 1000 mg/L; the inhibition was -52.1%, -105.9%, -78.2%, -74.8% and 0.5% for test item concentrations of 10, 32, 100, 320 and 1000 mg/L, respectively. - Results with reference substance (positive control):
- The 3-hour EC50 for total respiration was found to be 6.9 mg/L (lower cl: 4.0 mg/L, higher cl: 11.7 mg/L) thus lying in the range of 2 - 25 mg/L recommended by the test guidelines. The 3-hour EC50 for heterotrophic respiration was found to be 12.0 mg/L (lower cl: 2.6 mg/L, higher cl: 58.1 mg/L) thus lying in the range of 5 - 40 mg/L recommended by the test guidelines. For the oxygen uptake due to nitrification the 3-hour EC50 was 2.3 mg/L (lower cl: 1.0 mg/L, higher cl: 5.1 mg/L) lying in the range of 0.1 - 10 mg/L recommended by the test guidelines. The result confirms the suitability of the activated sludge used.
- Reported statistics and error estimates:
- For normal distribution of the data, the Shapiro-Wilk`s Test was used, the variance homogeneity was tested by the Levene`s Test. Subsequently, the Williams Multiple Sequential t-test Procedure (one-sided smaller) with Levenberg-Marquardt optimization was used to determine the 3-hour ECx and their 95 %-confidence limits and LOEC and NOEC values, if possible. For nitrification respiration of the test item, Dunnett’s multiple t-Test (one-sided smaller) with Levenberg-Marquadt optimization was used to determine the 3-hour ECx and their 95 %- confidence limits and LOEC and NOEC values if possible.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- NOEC: 320 mg/L (total respiration)
NOEC: <10 mg/L (heterotrophic respiration)
NOEC: ≥1000 mg/L (nitrification respiration) - Executive summary:
The effects of the test substance on activated sludge were determined in a 3-hour exposure laboratory study conducted under OECD Guideline 209. Activated sludge from the aeration tank of a municipal sewage treatment plant was exposed to the test substance at concentrations of 10, 32, 100, 320 and 1000 mg/L and 6 untreated controls. The same test design was repeated with the nitrification inhibitor Nallylthiourea. The test substance had no inhibiting toxic effects on the total respiration of the activated sludge up to the concentration of 320 mg/L. The inhibition was around 23% at concentrations of 1000 mg/L. Heterotrophic respiration of the activated sludge was only moderately inhibited up to concentrations of 1000 mg/L. No dose-related changes in inhibition were noticed.
The NOEC was determined to be 320 mg /L for total respiration, <10 mg /L for heterotrophic respiration and was determined to be ≥1000 mg/L for nitrification respiration.
The 3-hour EC50 could not be established for total, heterotrophic and nitrification respiration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.