Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-729-9 | CAS number: 87-19-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-10-04 to 2012-12-18
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois de Bay, Satigny, Switzerland) was used.
- Preparation of inoculum for exposure: The sludge is collected in the morning, washed three times in mineral medium (by centrifuging at 1000g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to guideline
- Additional substrate: no
- Solubilising agent: no
- Test temperature: 21.4°C-22.3°C
- pH of medium: 7.4 ± 0.2
- pH of medium adjusted: yes, with phosphoric acid or potassium hydroxide
- Suspended solids concentration: 1.53 g dw/L
TEST SYSTEM
- Number of culture flasks/concentration: 6
2 flasks with inoculum and test substance; conc. of test substance: 20 mg/l;
2 flasks with only inoculum;
2 flasks with inoculum and reference substance; conc. of reference substance: 99 mg/l;
SAMPLING
- Sampling frequency: daily
- Reported values: on days 1, 2, 3, 12, 21, 28 and 35
- Sampling method: every day, the oxygen consumption of each flask is recorded and correct temperature and stirring checked. At the end of the test period, the pH of each flask is measured again.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Abiotic sterile control: no
- Toxicity control: no - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80
- Sampling time:
- 28 d
- Remarks on result:
- other: More information on the results can be found at "Any other information on results incl. tables" field
- Details on results:
- Reference substance: degradation of the reference substance exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion). Moreover, the repeatability validity criterion (not more than 20% difference between replicates) is fulfilled for the flasks containing the test substance. Therefore, the test is considered valid.
Test item 10-day window: the criterion is fulfilled (15% biodegradation on day 2 and 75% on day 12). - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance undergoes 80% biodegradation after 28 days in the test conditions. The 10-day window criterion is also fulfilled (15% biodegradation on day 2 and 75% on day 12).
The test substance should be regarded as readily biodegradable according to this test. - Executive summary:
The biodegradability of the test item was examined according to the manometric respirometry test method C.4 -D and according to OECD guideline 301F. After 28 days, the biodegradation was found to be 80%.
The 10 -day window criterion is also fulfilled: 15% biodegradation on day 2 and 75% on day 12. Hence, the test item should be considered to be readily biodegradable. Moreover, the test substance did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
The degradation of the reference substance exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
Reference
Biodegradability: % biodegradation of test substance
replicate | days | ||||||
1 | 2 | 3 | 12 | 21 | 28 | 35 | |
1 | 6 | 6 | 42 | 75 | 80 | 78 | 77 |
2 | 5 | 24 | 46 | 75 | 79 | 81 | 80 |
mean | 5 | 15 | 44 | 75 | 79 | 80 | 79 |
Biodegradability: % biodegradation of reference substance
replicate | days | ||||
5 | 7 | 14 | 21 | 28 | |
1 | 72 | 77 | 85 | 89 | 88 |
2 | 78 | 81 | 95 | 98 | 97 |
mean | 75 | 79 | 90 | 93 | 93 |
Description of key information
Readily biodegradable (80% biodegradation after 28 days; OECD 301 F)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
In a Manometric Respirometry Test (OECD 301 F) the test item undergoes 80% biodegradation after 28 days. In this GLP test, the 10 -day window criterion is also fulfilled: 15% biodegradation on day 2 and 75% on day 12. Hence, the test item should be considered to be readily biodegradable. Moreover, the test substance did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
The degradation of the reference substance exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.