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EC number: 208-634-1 | CAS number: 536-45-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Toxicity control of the study Biodegradation in water OECD 301 F
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 - 31 Aug 2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data based on the inhibition control of a ready biodegradability study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The amounts of test item (102.7 mg/L) and reference item (104.1 mg/L) were directly weighed into the test flasks. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source of inoculum/activated sludge: Aerobic activated sludge from sewage treatment plant Bensheim, Germany
- Storage conditions: aerated
- Storage length: aerated for 2 days
- Preparation of inoculum for exposure: Activated sludge was washed by centrifugation and the supernatant liquid phase was decanted; solid material was resuspended in test water and centrifuged again and repeated three times; based on the the ration of wet sludge to its dry weight calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated for 2 days.
- Pretreatment: An aliquot of sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
- Concentration of sludge: 3.5 g dry material per litre - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Remarks on exposure duration:
- Test item is not inhibitory to aerobic activated sludge microorganisms since degradation was 30% after 14 days (and 46% after 28 d).
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: according the OECD guideline 301
- Test temperature: 22 ± 1 °C
- pH: 7.5 to 8.2
- Continuous darkness: yes
- Other: continuous stirring
TEST SYSTEM
- Culturing apparatus and measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany (Manometric Test System, 500 mL fill volume in flasks)
- Number of culture flasks/concentration: 2
- Details of trap for CO2: potassium hydroxide solution (45%)
SAMPLING
- Sampling method: change of pressure in the test flasks was measured by means of a manometric method
CONTROL AND BLANK SYSTEM
- Inoculum control: 2 replicates
- Abiotic sterile control: 1 replicate (poisoned with HgCl2)
- Toxicity control: 1 replicate
- Other: procedure control - 1 replicate - Reference substance (positive control):
- yes
- Remarks:
- sodium bezoate
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 102.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Result of toxicity control from ready biodegradability test
- Results with reference substance (positive control):
- Test item is not inhibitory to aerobic activated sludge microorganisms since degradation was >25% within 14 days (30% after 14 d and 46% after 28 d).
- Validity criteria fulfilled:
- yes
- Conclusions:
- NOEC (14d) = 102.7 mg/L (activated sludge, OECD 301 F)
Reference
The toxicity control attained 30% degradation after 14 days of incubation (based on ThODNH4 of Sodium anisate: 1.562 mg O2/mg test item and ThODNH4 of sodium benzoate: 1.666 mg O2/mg reference item).
“If in a toxicity test, containing both the test and reference substance, less than 35% degradation (based on total DOC) or less than 25% (based on total ThOD or ThCO2) occurred within 14 days, the test substance can be assumed to be inhibitory.” (OECD guideline 301)
Since more than 25% degradation occurred in the toxicity control, the substance is with high probability not toxic to aquatic microorganisms. The test item concentration in the toxicity control of 102.7 mg test item/L can be used as NOEC value for the toxicity to aquatic microorganisms.
Description of key information
NOEC (14d) = 102.7 mg/L (activated sludge, OECD 301 F)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 102.7 mg/L
Additional information
Since no studies on the toxicity to aquatic microorganisms are available a ready biodegradability study is used to derive a NOEC for the toxicity to aquatic microorganisms. If a compound degrades well in a ready biodegradability test, or does not inhibit the degradation of a positive control at a certain concentration, this concentration can be used as a NOEC value. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
A substance can be assumed to be not inhibitory to aquatic microorganisms, if in the toxicity control of a ready biodegradation test, more than 25% degradation based on ThOD occurred within 14 days (OECD guideline 301F). For the test item Sodium Anisate (CAS 536-45-8) a biodegradation test according to OECD guideline 301F is available. The test includes a toxicity control, which contains 102.7 mg/L of the test item and 104.1 mg/L mg/L of sodium benzoate. The toxicity control attained 30% degradation after 14 days of incubation. Hence, the substance is not toxic to aquatic microorganisms in the toxicity control and the test item concentration of 102.7 mg/L can be used as NOEC.
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