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EC number: 435-360-1 | CAS number: 144447-11-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 August 2011 to 17 August 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- Deviations:
- no
- Principles of method if other than guideline:
- not relevant
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 435-360-1
- EC Name:
- -
- Cas Number:
- 144447-11-0
- Molecular formula:
- C20H22O5
- IUPAC Name:
- 4-methoxyphenyl 4-[4-(oxiran-2-yl)butoxy]benzoate
- Details on test material:
- Identification: Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester
Chemical name: 4-methoxyphenyl-4(5,6-epoxyhexyloxy)benzoate
Molecular formula: C20H22O5
Molecular weight: 342.39
CAS Number: 144447-11-0
Description: White to off-white powder
Batch: 1001501002
Purity: 98.2%
Test substance storage: At room temperature in the dark
Stability under storage conditions: Stable
Expiry date: 01 February 2013
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Stability in water: Not indicated
Solubility in water: 0.586 mg/L (NOTOX project 497269)
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for possible analysis were taken from all test concentrations and the control according to the schedule below. In addition, filter containing undissolved residue was kept for possible analysis.
Frequency: at t=0 h and t=48 h
Volume: 2 ml from the approximate centre of the test vessels
Storage: Samples were stored in a freezer until analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The batch of Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester tested was a white to off-white powder with a purity of 98.2% and not completely soluble in test medium at the loading rate initially prepared.
Preparation of test solutions started with a loading rate of 100 mg/l applying a 2-day period of magnetic stirring to ensure maximum dissolution in test medium. The obtained mixture containing undissolved material was filtered through a 0.45 µm membrane filter (Whatman, RC55). The Water Soluble Fraction (WSF) was used as the highest test concentration. The lower test concentrations were prepared by subsequent dilutions of the WSF in test medium. The final test solutions were all clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Source: In-house laboratory culture with a known history.
- Age at study initiation: < 24 hours
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- 19.2-20.9
- pH:
- 7.9-8.0
- Dissolved oxygen:
- 8.9-9.1
- Salinity:
- not measured
- Nominal and measured concentrations:
- Nominal :
Solutions containing 0.1, 1.0, 10 and 100% of the filtrate prepared at a loading rate of 100 mg/l.
Measured:
The measured concentration in the sample taken from the undiluted filtrate at the start of the test was 0.35 mg/l. During the exposure period the measured concentration had decreased to 0.26 mg/l
(75% of initial).
Based on these results, the geometric mean exposure concentration was calculated to be 0.30 mg/l. - Details on test conditions:
- Test duration: 48 hours
Test type: Static
Test vessels: 100 ml, all-glass
Medium: Adjusted ISO medium
Number of daphnids: 20 each in the control and the highest concentration, 10 each in the remaining concentrations
Loading: 5 per vessel containing 80 ml of test solution
Light: 16 hours photoperiod daily
Feeding: No feeding
Aeration: No aeration of the test solutions.
Introduction of daphnids: Within 15 minutes after preparation of the test solutions. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: -
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: -
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: .
- Details on results:
- Behavioural abnormalities: no
- Immobility of control: no immobility was observed.
- Other adverse effects control: no
Behavioural abnormalities: no
- Immobility of control: no immobility was observed.
- Other adverse effects control: no - Results with reference substance (positive control):
- The 24h-EC50 was 0.58 mg/l with a 95% confidence interval between 0.53 and 0.68 mg/l.
The 48h-EC50 was 0.41 mg/l with a 95% confidence interval between 0.36 and 0.46 mg/l. - Reported statistics and error estimates:
- No EC50 could be calculated because the test substance proved to be non-toxic (EC50 > maximum soluble concentration tested).
Any other information on results incl. tables
Acute immobilisation of daphnids after 24 and 48 hours
Test substance1 % WSF |
Vessel number
|
Number Daphnia exposed |
Response at 24 h |
Response at 48 h |
||
number |
Total % |
number |
Total % |
|||
control |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 0 0 |
0 |
0.1 |
A B |
5 5 |
0 1 |
10 |
0 1 |
10 |
1.0 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
10 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
100 (0.30) |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 1 0 |
5 |
1. Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester
() – between brackets the average exposure concentration is given [mg/l]
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester did not induce acute immobilisation of Daphnia magna at a geometric mean concentration of 0.30 mg/l, i.e. an initially prepared loading rate of 100 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 was beyond the range tested, i.e. exceeded a geometric mean exposure concentration of 0.30 mg/l (an initially prepared loading rate of 100 mg/l).
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