Reaction mass of N,N-ethanediylbis- Hexadecanamide, N-[2-[(1-oxohexadecyl)amino]ethyl]-Octadecanamide, Hexadecanoic acid, 12-hydroxystearic acid, reaction products with ethylenediamine, N,N-ethanediylbis-Octadecanamide and Octadecanoic acid, 12-hydroxystearic acid, reaction products with ethylenediamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline Study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle: Tween 80

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Laboratory culture: No, activated sludge from a Municipal sewage treatment plant, Hildesheim
- Preparation of inoculum for exposure: 2 x washed with drinking water (dechlorinated)
- Initial biomass concentration: 2 g/L (dry weight) sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

21 °C

Nominal and measured concentrations:

Nominal concentrations: Control, 100, 180, 320, 580, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration : 1
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 1



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic effluent according to Guideline

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: EC50 = 47 %

The inhibition of the degradation activity of activated sludge is not anticipated, the EC10 is > 1000 mg/L.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Refer to chapter 13

Reason / purpose for cross-reference:

read-across source

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Validity criteria fulfilled:

yes

Conclusions:

The inhibition of the degradation activity of activated sludge is not anticipated (EC10 > 1000 mg/L).

Executive summary:

The toxicity of the Read Across Source substance Amides, C16-C18 (even), N,N'-ethylenebis to activated sludge was investigated in one study according to OECD 209 (Clariant, 1989). The results from this test show no effects on the respiration rate of activated sludge up to the highest nominal test concentration of 1000 mg/L. Therefore, disturbances in the biodegradation process of activated sludge is not anticipated (NOEC >= 1000 mg/L).

Description of key information

The inhibition of the degradation activity of activated sludge is not anticipated (EC10 > 1000 mg/L).

The toxicity of the Read Across Source substance Amides, C16-C18 (even), N,N'-ethylenebis to activated sludge was investigated in one study according to OECD 209 (Clariant, 1989). The results from this test show no effects on the respiration rate of activated sludge up to the highest nominal test concentration of 1000 mg/L. Therefore, disturbances in the biodegradation process of activated sludge is not anticipated (NOEC >= 1000 mg/L).