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EC number: 205-755-1 | CAS number: 150-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 March 2017 - 31 March 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N-bis(2-hydroxyethyl)glycine
- EC Number:
- 205-755-1
- EC Name:
- N,N-bis(2-hydroxyethyl)glycine
- Cas Number:
- 150-25-4
- Molecular formula:
- C6H13NO4
- IUPAC Name:
- N,N-bis(2-hydroxyethyl)glycine
- Reference substance name:
- N-(2-hydroxyethyl)ethylenediaminetriacetic acid
- EC Number:
- 205-759-3
- EC Name:
- N-(2-hydroxyethyl)ethylenediaminetriacetic acid
- Cas Number:
- 150-39-0
- Molecular formula:
- C10H18N2O7
- IUPAC Name:
- N-{2-[bis(carboxymethyl)amino]ethyl}-N-(2-hydroxyethyl)glycine
- Reference substance name:
- Not specified
- IUPAC Name:
- Not specified
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Dihydrogen oxide
- Test material form:
- solid: crystalline
- Details on test material:
- Appearance: white crystalline powder
Storage conditions: at room temperature protected from light
Constituent 1
impurity 1
impurity 2
impurity 3
- Specific details on test material used for the study:
- Solubility in water: 347 g/L (20°C) (see section 4.8)
Stability in water: not indicated
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Single samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
Frequency at t=0 h and t=48 h
Volume 3.6 mL from the approximate centre of the test vessels
Storage Samples were stored in a freezer (=-15°C) until analysis at the analytical laboratory of the test facility
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Correction for purity: no correction for purity was made.
- Method: Preparation of test solutions started with the highest concentration of 100 mg/L. No other treatment than vigorous shaking was needed to completely dissolve the test item in medium. Lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. All test solutions were clear and colorless at the end of the preparation procedure.
- Controls: test medium without test item or other additives.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, 1820
- Source: in-house laboratory culture with a known history. At least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions.
- Age of parental stock: Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
- Age at study initiation: < 24 hours, from parental daphnids of more than two weeks old.
- Feeding during test: no
ACCLIMATION
- Acclimation period: no
BREEDING:
- Method: each batch was started with newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium (M7) in an all-glass culture vessel. Cultures were max. 4 weeks old. After 7 days of cultivation half of the medium was renewed twice a week.
- Feeding: daily, a suspension of fresh water algae.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L
- Test temperature:
- 20°C
- pH:
- 7.3-7.9
- Dissolved oxygen:
- 9.0-9.2 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0.10, 1.0, 10 and 100 mg/L
Measured concentrations: 100 mg/L: 140 mg/L at t=0; 109 mg/L at t=48 - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Fill volume: 80 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4, for the highest test concentration, 2 for the other test concentrations
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Standard medium used: yes, adjusted ISO medium
- Source/preparation of dilution water: tap water purified by reverse osmosis
- Culture medium different from test medium: yes, M7 was used as culture medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: the test was performed in the dark
EFFECT PARAMETERS MEASURED: mobility at 24 and 48 hours.
- Additional measurements: pH and dissolved oxygen at the beginning and at the end of the test, in the highest test concentration and the control. Temperature of the medium: continuously in a temperature control vessel.
COMBINED LIMIT/RANGE-FINDING STUDY: yes, reported as the final test - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (Feb 2017)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 124 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobility was observed at any of the test concentrations after 24 hours. In the nominal concentration of 100 mg/L, 5% of the daphnids were immobilized after 48 hours of exposure.
- No biological, behavioural or other abnormalities were observed in the control group or any of the test concentrations.
- Analyses of the samples taken from the nominal concentration of 100 mg/L showed a concentration of 140 mg/L at the start of the test. The measured concentration decreased to 78% of initial at the end of the test (109 mg/L). Based on this result, a geometric average exposure concentration of 124 mg/L was used to determine effect parameters.
- Individual pH, temperature and dissolved oxygen values remained within acceptable limits throughout the duration of the study. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- 48h-EC50: 0.48 mg/L, 95%-confidence interval: 0.43-0.53 mg/L
- Other: results fell within the historical range.
Any other information on results incl. tables
Table 1 Measured concentrations in test samples
Time of sampling |
Concentration |
Relative to nominal |
Relative to initial |
|
Nominal |
Analyzed |
|||
0 |
0 |
0.05722 |
n.a. |
|
|
100 |
1403 |
140 |
|
48 |
0 |
n.d. |
n.a. |
n.a. |
|
100 |
109 |
109 |
78 |
1 Samples were stored in the freezer (= -15°C) until the day of analysis.
2 The maximum contribution to the sample at the lowest concentration level was 0.043%.
3 Estimated value, calculated by extrapolation of the calibration curve.
n.d. Not detected.
n.a. Not applicable.
Table 2 Number of introduced Daphnids and incidence of immobility
Time (h) |
Replicate |
Nominal concentration Bicine (mg/L) |
||||
Control |
0.1 |
1.0 |
10 |
100 |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
|
|
|
5 |
|
D |
5 |
|
|
|
5 |
|
Total introduced |
20 |
10 |
10 |
10 |
20 |
|
24 |
A |
0 |
0 |
0 |
0 |
0 |
B |
0 |
0 |
0 |
0 |
0 |
|
C |
0 |
|
|
|
0 |
|
D |
0 |
|
|
|
0 |
|
Total immobilised |
0 |
0 |
0 |
0 |
0 |
|
Effect % |
|
|
|
|
|
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
0 |
0 |
0 |
B |
0 |
0 |
0 |
0 |
0 |
|
C |
0 |
0 |
|
|
0 |
|
D |
0 |
0 |
|
|
1 |
|
Total immobilised |
0 |
0 |
0 |
0 |
1 |
|
Effect % |
0 |
0 |
0 |
0 |
5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- 1). In the control, no daphnids became immobilised or showed other signs of disease or stress. 2). The oxygen concentration at the end of the test was = 3 mg/L in control and test vessels (e.g., =9.1 mg/L).
- Conclusions:
- The 48h-EC50 of the substance towards Daphnia magna, exceeded a mean measured exposure concentration of 124 mg/L.
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