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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28 days
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
not specified
GLP compliance:
no
Remarks:
Lab not yet GLP accredited for this end point

Test material

Constituent 1
Chemical structure
Reference substance name:
Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
EC Number:
261-118-8
EC Name:
Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
Cas Number:
58096-47-2
Molecular formula:
C16H26O2
IUPAC Name:
decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
Test material form:
liquid
Specific details on test material used for the study:
CAS No: 58096-47-2
EC No: 261-118-8
Chemical Name: Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate
Trade Name: Clovanyl-3-Formate (CARYOLAN)
Molecular formula: C16H26O2
Molecular weight: 250.379

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic (adaptation not specified)
Details on inoculum:
Inoculum: Domestic sewage
Collected on: 04.12.2017
Treatment: Aeration and activation with 1 gm glucose / 1000ml every day
Check: Using bactyslide (Total bacterial count ~ 10 3 to 10 4 cells/ml
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Test Method: OECD 301F [Respirometric manometry]
Equipment: Lovibond BOD system BD600
Incubator Lovibond thermostatic cabinet
Inoculum: Domestic sewage
Collected on 04.12.2017
Treatment Aeration and activation with 1 gm glucose / 1000ml every day
Check: Using bactyslide (Total bacterial count ~ 10 3 to 10 4 cells/ml
Sample weight in test media: 150 mg
Volume of test media: 56 ml
ThOD of sample: 2.88 mg/mg
Test duration: 28 day
10 Day window: 19th to 28th Dec 2017
Test temperature: 22 ± 1 ˚C
Reference substance
Reference substance:
other: Glucose glutamate

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
ca. 5
Sampling time:
28 d
Remarks on result:
other: Not readily biodegradable
Details on results:
From the test results, it is concluded that the chemical is not readily bio-degradable.

Any other information on results incl. tables

The test chemical Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate was found to be not readily biodegradable.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The test item Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate was found to be not readily biodegradable under the test conditions.
Executive summary:

The test item Decahydro-1,1,7-trimethyl-3a,7-methano-3aH-cyclopentacyclooct-3-yl formate was found to be not readily biodegradable under the test conditions.