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EC number: 459-270-7 | CAS number: 2568-33-4 MBD
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 May 2005 - 15 July 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- On day 2 of the definitive test, the maximum temperature in the incubator was 25.1°C, which is
slightly above that stated in the study protocol (25°C). - Deviations:
- yes
- Remarks:
- As the validity criteria for this type of study were met this is not considered to have affected the integrity or validity of the study.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- On day 2 of the definitive test, the maximum temperature in the incubator was 25.1°C, which is
slightly above that stated in the study protocol (25°C). - Deviations:
- yes
- Remarks:
- As the validity criteria for this type of study were met this is not considered to have affected the integrity or validity of the study.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Appearance: Clear, colourless liquid
Storage conditions: Room temperature in the dark
Batch number: 52834
Expiry date: December 2006
Purity: 99% - Analytical monitoring:
- yes
- Details on sampling:
- For samples whose nominal concentration of the test item was greater than 25 mg/l, the aqueous samples were diluted with methanol (1 ml to 20 ml) to bring the expected concentration within the calibration range.
For samples whose nominal concentration of the test item was less than 25 mg/l, the aqueous samples were partially evaporated and then diluted with methanol (1 ml to 20 ml) to bring the expected concentration within the calibration range. - Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: The test media were prepared from aqueous dilution in which the test substance was added directely to algal culture medium. If further dilution was required, sterile culture medium was used.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: CCAP 278/4
- Age of inoculum (at test initiation): three days
- Method of cultivation: Sterile algal nutrient medium
ACCLIMATION
- Culturing media and conditions (same as test or not): yes - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24.2 ± 0.9°C
- pH:
- 7.80 - 10.30
- Nominal and measured concentrations:
- Nominal concentrations: 4.27, 9.39, 20.7, 45.5 & 100 mg/l
Mesured concentations: 3.45, 7.97, 16.0, 45.2, 95.8 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: Conical flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Conical flasks (250 ml)
- Initial cells density: 1 x 10^4 cells/ml (nominal)
- Control end cells density: 1 x 10^4 cells/ml (nominal)
- No. of organisms per vessel: 1 x 10^4 cells/ml (nominal)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous illumination
- Light intensity and quality: 6158 lux provided by “cool white”
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: haemacytometer
- Other: The presence of any abnormal cells
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study:The results of this test indicated that the test substance inhibited the growth of Selenastrum by 42% compared to the control group when prepared at a nominal concentration of 100 mg/l. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 95.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- not determinable
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 95.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 95.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 95.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- The measurements of water quality (temperature and pH) in control and test flasks remained within acceptable limits throughout the study.
- Results with reference substance (positive control):
- The results of the most recent laboratory reference test using potassium dichromate indicated that its 72-hour EbC50 to Selenastrum capricornutum was 0.92 mg/l; this was within the range typically obtained in this laboratory (0.3 to 1 mg/l).
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours of exposure to the test item, the EbC50 and ErC50, could not be determined but must be > 95.8 mg/l.
The “no observed effect concentration” (NOEC) was >95.8 mg/l.
Reference
Description of key information
After 72 hours of exposure to the test item, the EbC50 and ErC50, could not be determined but must be > 95.8 mg/l.
The “no observed effect concentration” (NOEC) was >= 95.8 mg/l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 95.8 mg/L
- EC10 or NOEC for freshwater algae:
- 95.8 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.