Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-140-7 | CAS number: 78-76-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 December 2016 - 12 January 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-bromobutane
- EC Number:
- 201-140-7
- EC Name:
- 2-bromobutane
- Cas Number:
- 78-76-2
- Molecular formula:
- C4H9Br
- IUPAC Name:
- 2-bromobutane
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in the report): JNJ-47961173-AAA
- Physical state: Liquid
- Colour: Colourless
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: M15IB3251
- Expiration date of the lot/batch:07 April 2017 (retest date)
- Purity: 100% (based on gas chromatographic purity)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature
- Stability under test conditions: stable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source: municipal sewage treatment plant receiving predominantly domestic sewage, 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands.
- Storage conditions: sludge was kept under continuous aeration until further treatment
- Preparation of inoculum for exposure: Before use, the sludge was allowed to settle (30 minutes) and the supernatant liquid was used as inoculum.
- Pretreatment: no
- Concentration of sludge: the concentration of suspended solids was determined to be 5 g/L in the concentrated sludge.
- Water filtered: tap-water pruified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon - Duration of test (contact time):
- ca. 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 57 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Details on study design:
- TEST CONDITIONS
- Composition of medium: test water prepared according to test guidelines, analytical grade salts dissolved in tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon.
* mineral stock solution A: 8.5 g KH2PO4, 21.75 g K2HPO4, 67.20 g Na2HPO4.12H2O, 0.5 gNH4Cl dissolved in 1 L Milli-Q water, pH 7.4 ± 0.2
* mineral stock solution B: 22.50 g MgSO4.7H2O dissolved in 1 L Milli-Q water
* mineral stock solution C: 36.4 g CaCl2.2H2O dissolved in 1 L Milli-Q water
* mineral stock solution D: 0.25 g FeCl3.6H2O dissolved in 1 L Milli-Q water
* Final test medium: 10 mL of solution A and 1 mL of solutions B, C and D per L of test medium
- Additional substrate: no
- Test temperature: 20-22°C
- pH: 7.3-7.7, measured prior to testing.
- pH adjusted: no
- Aeration of dilution water: The test was performed in sealed bottles with a headspace of air.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Sealed glass serum bottles with capacity of 160 mL
- Number of culture flasks/concentration: 2
* test substance and inoculum: 5 replicates
* inoculum blank: 5 replicates
* positive control: 3 replicates
* toxicity control: 3 replicates
- Abiotic control: containing untreated medium, test item and sterilising agent
- Measuring equipment: Each sample was analysed in triplicates using Shimadzu TOC-VCPH total organic carbon analyzer combined with a Shimadzu ASI-V autosampler.
SAMPLING
- Sampling frequency: all the solutions were sampled on day 1, 7 and 14. Inoculum blank and test item additionally on day 21 and 28.
- Sampling method: 7 M NaOH was injected into each bottle, after which they were shaken for 1 h. The bottles were removed from the shaker and allowed to settle and the supernatant was used for IC analysis.
CONTROL AND BLANK SYSTEM
The test consisted of five groups:
- Inoculum blank: containing inoculated medium.
- Procedure control: containing inoculated medium and reference item.
- Test item: containing inoculated medium and test item.
- Toxicity control: containing inoculated medium, reference item and test item.
- Abiotic control: containing untreated medium, test item and sterilising agent
Reference substance
- Reference substance:
- other: 1-Octanol
Results and discussion
- Preliminary study:
- -The mean amount of TIC present in the blank controls at the end of the test was < 3 mg C/L (1.8 mg C/L).
- Test performance:
- The mean percentage degradation of the reference item has reached the level for ready biodegradability (>60%) by 14 days (83%). The mean amount of TIC present in the blank controls at the end of the test was < 3 mg C/L (1.8 mg C/L). Since all criteria for validity of the test were met, this study was considered to be valid.
Between 24-Dec-2016 and 27-Dec-2016 the temperature has periodically been above the range as stated in the study plan (20±1°C). Evaluation: The temperature has only been above the specified range momentarily, and never for longer than 15 minutes. Since all validity criteria were met, it can be stated that this deviation has had no significant impact on this study.
% Degradation
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 19
- Sampling time:
- 28 d
- Remarks on result:
- other: relative biodegradation values calculated from the IC measurements
- Details on results:
- -The criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
BOD5 / COD results
- Results with reference substance:
- -The reference item has reached the level for ready biodegradability (>60%) by 14 days (83%) confirming the suitability of the medium and test conditions.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The relative biodegradation values calculated from the IC measurements performed during the test period revealed 19% biodegradation of JNJ-47961173-AAA (2-bromobutane). Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met. Based on the results of the test, it can be concluded that JNJ-47961173-AAA (2-bromobutane) was not readily biodegradable. The results of the test can be considered reliable without restriction.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.