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EC number: 203-380-8 | CAS number: 106-27-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-08-26 to 2015-08-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- dated 2013-11-05
- Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: All concentration levels and the control were analytically verified via SPME-GC-FID at the start (0 and 24 hours) and at the end of the exposure (24 and 48 hours)
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (100 mg of the test item in 1L ISO test water) was prepared and stirred at approximately 1100 rpm for 3 hours
- Controls:ISO test water without test item - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Clone 5
- Source: Institute for Water, Soil and Air quality (WaBoLu) in Berlin, Germany
- Feeding during test: No
- Food type: A mix of unicellular algae (e.g., Pseudokirchneriella subcapitata)
- Frequency: At least 5 times per week
- Culture medium: Modified Elendt M4 medium according to Elendt (1990)
ACCLIMATION
- Age at study initation: 2 - 24 hours
- Acclimation period: At least 2 hours in ISO test water
- Acclimation conditions (same as test or not): Same as test - Test type:
- semi-static
- Water media type:
- other: ISO test water according to OECD 202
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Renewal of test item after 24 hours
- Hardness:
- 262 - 267 mg CaCO3/L start of exposure intervals
- Test temperature:
- 20.3 - 20.5 °C start of exposure intervals
- pH:
- 7.66 - 8.18 start of exposure intervals
7.14 - 7.89 end of exposure intervals - Dissolved oxygen:
- 8.54 - 8.77 mg/L start of exposure intervals
4.74 - 8.43 mg/L end of exposure intervals - Conductivity:
- 657 - 672 µS/cm start of exposure intervals
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 6.25, 12.5, 25.0, 50.0, 100 mg/L
Measured concentrations: 0 (control), 3.88, 7.90, 16.3, 35.0, 74.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks
- Type: closed
- Material, size, headspace, fill volume: approx. 130 mL test volume without headspace
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): one time after 24 hours each
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO test water according to OECD 202 (2004)
- Conductivity: 672 µS/cm (0 hours), 657 µS/cm (24 hours)
- Culture medium different from test medium: yes (culture medium: Elendt M4; test medium: ISO test water)
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light (max. 1340 lx)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Number of immobile daphnids after 24 hours and 48 hours
RANGE-FINDING STUDY
- Test concentrations: 1.00, 10.0 and 100 mg/L
- Results used to determine the conditions for the definitive study: Considering 100% immobilisation of the daphnids at 100 mg/L and 0% immobile daphnids below this value, more test concentrations between 10 and 100 mg/L were used, namely 6.25, 12.5, 25, 50 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 7.36 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval: 6.85 - 7.55 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.12 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 7.99 - 8.26 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 16.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.88 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The EC50 value of 2.00 mg/L of the reference item potassium dichromate after 24 hours was within the prescibed concentration range of 0.6 - 2.4 mg/L of quality criteria according to AQS P 9/2 (2000) for daphnids cultured in M4 medium. The EC50 value was also within the recommended range of 0.6 - 2.1 mg/L according to OECD 202 (2004).
- Validity criteria fulfilled:
- yes
- Conclusions:
- An Acute Immobilisation Test with Daphnia magna was carried out according to OECD 202 and under GLP conditions to determine the effect values (e.g., EC50) of the test substance after 24 and 48 hours exposure. After 48 hours exposure, an EC50 value of 8.12 mg/L (geometric mean measured) was calculated.
Reference
Table 1: Results after 24 and 48 hours exposure
Nominal concentration (mg/L) |
Geometric mean measured concentration (mg/L) |
Immobilisation (%) |
Temperature (°C) after 24 hours |
Dissolved oxygen concentration (mg/L) after 48 hours (arithmetic mean) |
pH value after 48 hours (arithmetic mean) |
|
24 hours |
48 hours |
|||||
0 (control) |
0 (control) |
0 |
0 |
20.5 |
8.00 |
7.85 |
6.25 |
3.88 |
0 |
0 |
- |
8.19 |
7.87 |
12.5 |
7.90 |
10 |
35 |
- |
7.96 |
7.82 |
25.0 |
16.3 |
70 |
100 |
- |
6.58 |
7.58 |
50.0 |
35.0 |
40 |
100 |
- |
7.16 |
7.65 |
100 |
74.6 |
100 |
100 |
- |
- |
- |
Description of key information
EC50: 8.12 mg/L (geometric mean measured).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 8.12 mg/L
Additional information
The toxicity of Isoamyl butyrate on Daphnia magna was determined in a reliable GLP study according to OECD 202. Individual of D. magna were exposed to 0 (control), 6.25, 12.5, 25.0, 50.0, 100 mg/L of the test substance, equivalent to geometric mean measured concentrations of 0 (control), 3.88, 7.90, 16.3, 35.0, 74.6 mg/L for 48 hours. Concentrations were analytical verified via SPME-GC-FID. After 48 hours exposure, an EC50 value of 8.12 mg/L (geometric mean measured) was estimated.
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