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EC number: 259-470-2 | CAS number: 55067-15-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-02-14 to 1990-03-12
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- 5 strains tested but not including E. coli
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1983
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-[4-[[2-(cyanoimino)hexahydro-4,6-dioxo-5-pyrimidyl]azo]phenyl]-6-methylbenzothiazole-7-sulphonic acid, compound with 2,2',2''-nitrilotris[ethanol] (1:1)
- EC Number:
- 259-470-2
- EC Name:
- 2-[4-[[2-(cyanoimino)hexahydro-4,6-dioxo-5-pyrimidyl]azo]phenyl]-6-methylbenzothiazole-7-sulphonic acid, compound with 2,2',2''-nitrilotris[ethanol] (1:1)
- Cas Number:
- 55067-15-7
- Molecular formula:
- C19 H13 N7 O5 S2 . C6 H15 N O3
- IUPAC Name:
- 2-[4-[[2-(cyanoimino)hexahydro-4,6-dioxo-5-pyrimidyl]azo]phenyl]-6-methylbenzothiazole-7-sulphonic acid, compound with 2,2',2''-nitrilotris[ethanol] (1:1)
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Op. 604/89
- Expiration date of the lot/batch: December, 1991
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in solvent: room temperature, protected from light
- Stability under test conditions: 2 hours stable in water and DMSO
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium strain TA 1535, TA 1537, TA 1538, TA 98, TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction from rat liver, Aroclor 1254 induced
- Test concentrations with justification for top dose:
- 10.0; 100.0; 333.3; 1000; and 5000 µg/plate
- Vehicle / solvent:
- - Vehicle/solvent used: deionized water
- Justification for choice of solvent/vehicle: good solubility of the test substance in water
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylene-diamine
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 72 hours at 37 °C
- Expression time (cells in growth medium): 72 hours at 37 °C
SELECTION AGENT (mutation assays): histidine
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: all revertant cells
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- A test article is considered as mutagen if in strain TA 100 the number of reversions is at least twice as high and in strains TA 1535, TA 1537, TA 1538, and TA 98 it is at least three times higher as compared to the spontaneous reversion rate.
Also, a dose-dependent increase in the number of revertants is regarded as an indication of possibly existing mutagenic potential of the test article regardless whether the highest dose induced the above described enhancement factors or not. - Statistics:
- No appropriate statistical method is available.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1: Number of revertants per plate (mean of three plates), experiment 1
strain TA 1535 |
strain TA 1537 |
Strain TA 1538 |
strain TA 98 |
strain TA 100 |
||||||
conc. [µg] |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
Negative control |
6 |
7 |
5 |
5 |
19 |
18 |
18 |
23 |
73* |
82 |
Solvent control |
7 |
8 |
4 |
5 |
16 |
19 |
17 |
22 |
79 |
75 |
10 |
8 |
7 |
5 |
5 |
14 |
18 |
19 |
26 |
77 |
68 |
100 |
5 |
5 |
4 |
6 |
15 |
17 |
19 |
26 |
71 |
74 |
333.3 |
5 |
8 |
5 |
3 |
18 |
20 |
19 |
21 |
72 |
57 |
1000 |
6 |
6 |
4 |
6 |
16 |
18 |
17 |
27 |
69 |
53 |
5000 |
5 |
7 |
2 |
5 |
17 |
20 |
19 |
26 |
73 |
70 |
Positive control sodium azide (10 µg) |
738 |
|
146 |
|
1475 |
|
760 |
|
656 |
|
Positive control 2-Aminoanthracene (10 µg) |
|
200 |
|
37 |
|
78 |
|
1440 |
|
1236 |
*Unexpected low revertant rate
Table 2: Number of revertants per plate (mean of three plates), experiment 2
strain TA 1535 |
strain TA 1537 |
Strain TA 1538 |
strain TA 98 |
strain TA 100 |
||||||
conc. [µg] |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
-MA |
+MA |
Negative control |
21 |
16 |
6 |
8 |
15 |
17 |
15 |
30 |
75 |
99 |
Solvent control |
21 |
17 |
6 |
9 |
21 |
23 |
15 |
27 |
78 |
88 |
10 |
29 |
21 |
7 |
6 |
22 |
16 |
18 |
27 |
71 |
79 |
100 |
19 |
22 |
7 |
9 |
21 |
30 |
14 |
24 |
66 |
84 |
333.3 |
16 |
21 |
4 |
10 |
21 |
30 |
13 |
31 |
73 |
88 |
1000 |
18 |
20 |
5 |
10 |
22 |
32 |
15 |
32 |
67 |
94 |
5000 |
17 |
16 |
6 |
8 |
24 |
22 |
16 |
43 |
71 |
80 |
Positive control sodium azide (10 µg) |
1335 |
|
217 |
|
1345 |
|
1522 |
|
1291 |
|
Positive control 2-Aminoanthracene (10 µg) |
|
65 |
|
107 |
|
547 |
|
1667 |
|
573 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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