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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no analytical follow-up; but the results demonstrate the adequacy of the test system

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(5-oxo-L-prolinato-N1,O2)copper
EC Number:
239-471-4
EC Name:
Bis(5-oxo-L-prolinato-N1,O2)copper
Cas Number:
15454-74-7
Molecular formula:
C10H12CuN2O6
IUPAC Name:
bis(5-oxo-L-prolinato-N1,O2)copper

Sampling and analysis

Analytical monitoring:
no
Remarks:
Total organic carbon follow-up was attempted, but failed due to barely detectable values

Test solutions

Vehicle:
yes
Remarks:
reconstituted water with Ca, Mg, Na, K supplies

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
between 140 and 250 mg CaCO3/L
Test temperature:
20°C +/- 2°C
pH:
8-8.3 at t0
Dissolved oxygen:
> 3mg/L
Nominal and measured concentrations:
nominal: 0, 0.1, 0.17, 0.31, 0.56, 1 mg/L
analysed: not applicable
Reference substance (positive control):
not required

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.155 mg/L
Basis for effect:
mobility
Details on results:
% of immobilized Daphnie in control: 0.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Daphnia magna 48h-EC50 (static assay, mobility) is 0.155 mg/L.