Registration Dossier
Registration Dossier
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EC number: 202-675-9 | CAS number: 98-51-1
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
- Principles of method if other than guideline:
- Oxygen Consumption Test (Huels Method). Oxygen concentration of controls and test replicates are compared after 4 - 6 hours of incubation.
- GLP compliance:
- no
Test material
- Reference substance name:
- 4-tert-butyltoluene
- EC Number:
- 202-675-9
- EC Name:
- 4-tert-butyltoluene
- Cas Number:
- 98-51-1
- Molecular formula:
- C11H16
- IUPAC Name:
- 1-tert-butyl-4-methylbenzene
- Details on test material:
- - Name of test material (as cited in study report): p-tert-butyltoluene
- Analytical purity: 96.3 %
- Lot/batch No.:none, Reaktor C510
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by eThe test substance was emulsified in Nonylphenol (10 EO, 5 PO)
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: obtained from Dr. Reinhard Kanne, Bayer AG, Leverkusen, Germany
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 4.58 h
Test conditions
- Test temperature:
- 24.7 - 25.3 °C
- Nominal and measured concentrations:
- Nominal test concentrations:
1, 10, 100 and 1000 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flask (100 ml)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 5 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 5 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 5 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
