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EC number: 218-507-2 | CAS number: 2167-23-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting date: 15 February 2000 Experimental completion date: 15 February 2000.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- protocol: 2,2-DI-(T-BUTYLPEROXY)BUTANE IN ISODODECANE
- labelling: LUPEROX 220EM50
- Elf Atochem trade name: LUPEROX 220 M50
All denominations correspond to the same test substance.
. batch number:
- protocol and labelling: 1 1579-99 10- 102
. description: colourless liquid
. composition:
- peroxide content: approximately 50%
- isododecane content: approximately 50%
density: 0.8 10 at 20°C
container: one plastic flask
date of receipt: 1 December 1999
storage conditions: at room temperature.
Data relating to the characterization of the test substance are documented in an analytical certificate (presented in appendix 1) provided by the Sponsor. - Analytical monitoring:
- no
- Details on sampling:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/l.
three test substance solutions: activated sludge in dechlorinated water (at 1.6 g/I) and test substance (to obtain final concentrations of 2, 10 and 20 mg/I in 2,2-DI-(T-BUTYLPEROXY)BUTANE).
three reference substance (3,5-dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/l) and reference substance (to obtain final concentrations of 4, 12 and 36 mg/l). - Vehicle:
- no
- Details on test solutions:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/l.
three test substance solutions: activated sludge in dechlorinated water (at 1.6 g/I) and test substance (to obtain final concentrations of 2, 10 and 20 mg/I in 2,2-DI-(T-BUTYLPEROXY)BUTANE).
three reference substance (3,5-dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/l) and reference substance (to obtain final concentrations of 4, 12 and 36 mg/l). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: activated sludge collected from a water treatment works containing effluent from a predominantly domestic origin.
Origin: the water treatment plant Emeraude (SIARR) (76141 Petit-Quevilly, France).
Reason for this choice: activated sludge is recommended in OECD guideline for this test.
Preparation: the inoculum was left to settle and the supernatant rejected. It was then filtered (to remove the biggest particules) and washed with dechlorinated water. Four samples of 50 ml were taken to determine the content in suspended matter (dry weight). The concentration of sludge (8.80 g/l) was then adjusted to 4 g/1 by addition of the appropriate volume of dechlorinated water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 ± 20 mg/I as CaCO3
- Test temperature:
- 22°C
- pH:
- 6.0-8.0
- Nominal and measured concentrations:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/l.
three test substance solutions: activated sludge in dechlorinated water (at 1.6 g/I) and test substance (to obtain final concentrations of 2, 10 and 20 mg/I in 2,2-DI-(T-BUTYLPEROXY)BUTANE).
three reference substance (3,5-dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/l) and reference substance (to obtain final concentrations of 4, 12 and 36 mg/l). - Details on test conditions:
- Reference substance
The reference substance 3,5-dichlorophenol used in the study was supplied by Sigma-Aldrich Chimie, 38297 Saint-Quentin-Fallavier, France.
The reference substance was identified as follows:
. name:
- protocol and labelling: 3,5-Dichlorophenol, 97%
. batch number:
- protocol and labelling: 00410-047
- analytical certificate: 00410
Both batch numbers correspond to the same substance according to a statement dated
2 1 October 1998 supplied by Sigma-Aldrich Chimie.
description: white crystalline powder
quantity and container: 10 g in one smoked glass flask
date of receipt: 22 September 1998
storage conditions: at room temperature and protected from light
purity: 99.4%.
Data relating to the characterization of the reference substance are documented in an analytical
certificate (presented in appendix 1) provided by Sigma-Aldrich Chimie.
Other substances
To prepare the reference substance:
sodium hydroxyde, batch No. 99064 (Prolabo, 94126 Fontenay-sous-Bois, France),
. sulfuric acid, batch No. 9819 1 (Prolabo, 94126 Fontenay-sous-Bois, France).
Preparation
Each test solution was prepared by direct addition of the test substance in the test flask with an appropriate micropipette (the volume added was based on the density) immediately before the start of the test.
The reference solution was prepared by dissolving 0.5 g of the reference substance, 3,5-Dichlorophenol, in 10 ml of NaOH at 1 N. The obtained preparation was then diluted to 30 ml with purified water. Aliquots of H2S04 at 1 N were added while stirring until the point of incipient precipitation was obtained. The solution was made up to 1000 ml using purified water.
The stock solution was agitated for 18 hours. The pH was 7.9 after agitation.
Environmental conditions during culture
The inoculum was maintained under agitation for one day before the test. As the sludge was not used immediately, 50 ml of sewage feed were added, per litre, just before agitation.
Synthetic sewage feed was prepared using dechlorinated water and analytical grade reagents following OECD recommendations.
Temperature: between 20 ± 2°C. The temperature in the culture room was recorded continuously and records retained.
Illumination: the stock vessel was kept away from light by wrapping it in opaque material.
Aeration: clean air was passed through the inoculum at the rate of 0.5 to 1 I/min until use.
pH: was checked before use. The inoculum was buffered, if necessary, to pH 6.0 to 8.0 using sodium bicarbonate solution.
Environmental conditions during the test
Temperature: 22°C.
Illumination: no special precautions were taken.
Dilution water: dechlorinated tap water was used, with a hardness of 280 ± 20 mg/I as CaCO3
Duration of test: 3 hours.
Aeration: air was bubbled through each solution at the rate of 0.5 to 1 I/min until the start of oxygen concentration measurements.
Test system
Groups of test and reference solutions and controls were set up as follows:
two controls containing inoculum at 1.6 g/l,
three test concentrations containing 2,2-DI-(T-BUTYLPEROXY)BUTANE at 2, 10 and 20 mg/I and inoculum at 1.6 g/l,
three reference concentrations containing the reference substance (3,5-Dichlorophenol) at 4, 12 and 36 mg/I and inoculum at 1.6 g/1.
The two control solutions and the three reference concentrations were also those of the CIT Study No. 18957 EAS but were attached to this study.
These solutions were prepared in test flasks and then immediately aerated for 3 hours before being transferred to an oxygen measuring apparatus.
Measurement of oxygen concentration of the above test solutions was determined using an oxygen probe placed into an opaque BOD flask filled with the test or reference solution which was agitated with a magnetic stirrer. The oxygen probe was connected to a meter and thence to a chart recorder.
Test method
Each concentration was prepared separately by adding 16 ml of sewage feed to each of the flasks which were made up to 300 ml with water only in the case of the controls or a nominal mixture of water and test substance (added directly using a micropipette) in the case of the test solutions.
Each mixture was then made up to 500 ml by adding 200 ml of inoculum. The solutions were aerated at between 0.5 to 1 litre of air per minute using a Pasteur-pipette as an aeration device.
Solutions were prepared approximately every 15 minutes for test times of 3 hours.
In order to verify the quality of the inoculum, the controls were prepared at the beginning (first control) and the end (second control) of the test.
After 3 hours of aeration/contact time, the content of the test flask was poured into the measuring apparatus and the oxygen concentration was determined during a few minutes. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Respiration rates of the test and reference solutions are presented in table 1.
The respiration rate of the test solution at the highest concentration in 2,2-DI(T-BUTYLPEROXY)BUTANE (20 mg/1) was equivalent to the respiration rate of the first control (i.e. these rates were within 15% of each other). Therefore, the oxygen consumption rate of the two other test solutions was not determined.
The EC50 calculation of the reference substance is presented in appendix 2.
Based on the inhibition percentage of the respiration rate obtained in the test solution of highest concentration, the 3-hour EC10 of the test substance was also> 20 mg/l (1). - Results with reference substance (positive control):
- As the difference between the two controls was below 15% and the EC50 of 3,5-dichlorophenol was between 5 and 30 mg/I, the test was considered valid.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under our experimental conditions, the 3-hour EC50 of the test substance, expressed as 2,2-DI-(T-BUTYLPEROXY)BUTANE, is >20 mg/l for activated sludge.
- Executive summary:
At the request of Elf Atochem SA, Paris-la-Defense, France, the acute toxicity of the test substance, 2,2-DI-(T-BUTYLPEROXY)BUTANE IN ISODODECANE, on micro-organisms present in activated sludge was evaluated using a 3-hour static test according to OECD guideline (No. 209, 4 April 1984).
Methods
The test solutions were prepared as follows:
two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/l,
three test substance solutions: activated sludge in dechlorinated water (at 1.6 g/I) and test substance (to obtain final concentrations of 2, 10 and 20 mg/I in 2,2-DI-(T-BUTYLPEROXY)BUTANE),
three reference substance (3,5-dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/l) and reference substance (to obtain final concentrations of 4, 12 and 36 mg/l).
The oxygen consumption of these solutions was measured for a few minutes, with a chart recorder, after an aeration of 3 hours.
The EC50 (concentration expected to cause 50% inhibition of the respiration rate) of the test substance and the reference substance was determined considering the oxygen consumption of the controls as 100%.
Results
As the validity criteria were met (the difference between the two controls was below 15% and the EC50 of 3,5-dichlorophenol was between 5 and 30 mg/l), the test was considered valid.
The respiration rate of the test solution at the highest concentration in 2,2-DI(T-BUTYLPEROXY)BUTANE (20 mg/l) was equivalent to the respiration rate of the first control (i.e. these rates were within 15% of each other). Therefore, the oxygen consumption rate of the two other test solutions was not determined.
EC50s, based on nominal concentrations, were as follows:
Substance
3-hour EC50 (mg/l)
95% confidence limits (mg/l)
Test substance
>20 (1)
-
Reference substance
14
12-17
The 3-hour EC10 of the test substance was also> 20 mg/l ( 1).
(1) Expressed as 2,2-DI-(T-BUTYLPEROXY)BUTANE
Conclusion
Under our experimental conditions, the 3-hour EC50 of the test substance, expressed as 2,2-DI-(T-BUTYLPEROXY)BUTANE, is > 20 mg/l for activated sludge.
Reference
EC50s, based on nominal concentrations, were as follows:
Substance |
3-hour EC50 (mg/l) |
95% confidence limits (mg/l) |
Test substance |
>20 (1) |
- |
Reference substance |
14 |
12-17 |
Table 1: Respiration rate in mg O2.l-1.h-1
|
Concentration (mg/l) |
Respiratory rate (mg O2.l-1.h-1) |
Percent inhibition (%) |
Control 1 |
0 |
33.6 |
|
Control 2 |
0 |
33.0 |
|
Reference substance |
4 |
30.9 |
7 |
12 |
16.0 |
52 |
|
36 |
6.9 |
79 |
|
Test substance (1) |
2 |
ND |
|
10 |
ND |
|
|
20 |
33.0 |
1 |
(1) expressed as 2,2-DI-(T-BUTYLPEROXY)BUTANE
ND: not determined
REFERENCE SUBSTANCE: 3-HOUR EC50 ESTIMATION
Concentration (mg/l) |
Percentage inhibition |
4 |
7 |
12 |
52 |
36 |
79 |
Slope: 1.002 Equation: Y = 1.002 x X + 2.354
3-hour EC50 |
Lower limit |
|
Upper limit |
14 |
(12 |
- |
17) |
mg/l
Significance: 95%
Description of key information
Toxicity to microorganisms was determined in an activated sludge respiration inhibition test was performed according to OECD 209 under GLP.
The EC50 of 2,2-di(tert-butylperoxy)butane for activated sludge after 3 hours contact time is greater than 20 mg/L. The EC10 is also greater than 20 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 20 mg/L
Additional information
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