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Diss Factsheets
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EC number: 201-891-0 | CAS number: 89-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The LC50 on embryonic stages of zebrafish (Danio rerio) after 96h of exposure to the test item under semi-static conditions was calculated to be 14 mg/L (confidence interval 13.7-14.3 mg/L). The highest tested concentration with no mortality was 4.47 mg/L and the lowest concentration with 100% mortality was 53.4 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 14 mg/L
Additional information
The acute toxicity of the test item on embryonic stages of zebrafish (Danio rerio) was tested according to the OECD guideline 236 and GLP.
With regard to the test item properties, the study was conducted under semi-static conditions and in the dark. 20 eggs were exposed to the following nominal concentrations of the test item 2.13 - 4.70 - 10.3 - 22.7 - 50 mg/L and to the reference substance. For the control 48 eggs were used including 4 internal control per tested concentration plate. The followig effects coagulation of embryos, absence of somite formation, non-detachment of the tail or lack of heart beat are considered as lethal endpoints which indicate mortality.
The concentrations of the test item were verified by LC-MS/MS at the start of the exposure (0 and 72 h) and at the end of exposure interval (24 and 96h) in all tested concentrations and the control. The measured concentrations were within 102 to 119% of the nominal concentrations at the start of the exposure. At the end of the exposure intervals (24h and 96h) they strongly decreased with the decreasing nominal concentrations resulting in a range of 23% to 105% of nominal values. The geometric mean measured concentrations were calculated to be : 1.43 - 4.47 10.4 - 23.1 - 53.4 mg/L.
Water quality parameters (pH, oxygen saturation and temperature) measured at the start and at the end of exposure were within the acceptable limits. The validity criteria of the OECD Guideline 236 were fulfilled.
The NOEC of the test item was 4.47 mg/L. The LC50 on embryonic stages of zebrafish after 96h of exposure to the test item under semi-static conditions was calculated to be 14 mg/L ( Confidence interval : 13.7 - 14.3 mg/L).
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