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EC number: 946-877-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 18 to 22 November (preliminary); from 2 to 6 December, 1996 (main)
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 17 July, 1992
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: Guppy
- Length at study initiation: 1 - 3 cm
- Fasting before test: fish were last fed 24 hours before the start of the definitive test.
BREEDING
- Breeding: parental and young fish were held in two 250 l glass aquaria.
- Illumination: 14 h a day
- temperature: 23 ± 2 °C
- Medium: continuosly aerated and filtered tap water from Schlleren.The bottom of the aquaria was covered with gravel (less than 1.5 cm). Every month, one third of the total volume of the aquaria was replaced by fresh tap water.
- Feeding frequency during acclimation: Sera micropam, Sera vipan, daily. The
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: fish were held in the batch used for the test.
- Health: mortality was less than 0.1 %. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- Test vessels: 21 - 22
Control:: 21 - 22 - pH:
- Test vessels: 8.0 - 8.8
Control: 8.0 - 8.6 - Dissolved oxygen:
- Test vessels: 6.2 - 8.6 mg/l
Control: 6.3 - 8.3 mg/l - Nominal and measured concentrations:
- 4, 8, 16, 32 and 64 mg/l, nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 l aquaria, all-glass.
- Aeration: aerated.
- No. of organisms per concentration: 7 fish.
- No. of vessels per concentration: one replicate.
- No. of vessels per control: one replicate.
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: tap water from Zürich-Schkkeren.
OTHER TEST CONDITIONS
- Photoperiod: 14 hours a day, supplied by overhead white fluorescent tubes.
EFFECT MEASURED
Fish were considered to be dead when they showed no reaction after touching the caudal fin or no visible breathing movements.
Observations: were made after 2, 24, 48, 72 and 96 hours of exposure.
EFFECT PARAMETERS MEASURED
Oxygen, pH, temperature: prior to the addition of fish to the test medium and after 2, 24, 48, 72 and 96 hours of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0
- Range-finding: prior to the definitive test a range-finding test with concentrations of 1, 10 and 100 mg/l and 3 fish per test vessel was conducted. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The no-observed effect concentration (NOEC) was 8 mg/l since the one fish that died was considered not to represent a significant effect as defined by the validity criteria of the test.
100 % mortality (LC100) was seen at the nominal concentration of 32 mg/l.
The following mortalities (of a total of 7 fish) were observed in the definitive test at the different test substance concentrations after 96 hours of exposure: 64 mg/l (7), 32 rng/l (7),16 mg/l (4), 8 mg/l (1) and g mg/l (1).Non mortality was observed in the control after 96 h of expesure.
Other toxic effects than mortality, e.g. loss of oordination, hypoactivity and swimming on the back, were not observed.
RANGE FINDING
100 % mortality was observed at 100 mg/l after 4 hours of exposure. 33 % mortality was observed at 10 mg/l after 48 hours of exposure. No mortality was observed at 1 mg/l and in the control after 96 hours of exposure. - Sublethal observations / clinical signs:
Incidence of dead fish observed at different points in time after start of exposure.
Nominal concentration (mg/l) Number of dead fish observed at different points in time after start of exposure 2 hrs 24 hrs 48 hrs 72 hrs 96 hrs Control 0 0 0 0 0 0 Test item 4 0 1 0 0 0 Test item 8 0 1 0 0 0 Test item 16 0 1 2 1 0 Test item 32 3 4 -a) -a) -a) Test item 64 6 1 -a) -a) -a) a) All fish died
- Conclusions:
- LC50 (96h): 14.3 mg/l (nominal)
- Executive summary:
The acute lethal toxicity (LC50) of test item to Poecilla reticulata (Guppy) was investigated under static exposure conditions over a period of 96 hours. 7 fish per concentration were exposed to the test substance. The nominal concentrations at test item as selected by a range finding test were 4, 8, 16, 32 and 64 mg/l, respectivety. The individual test concentrations were prepared by dilution of a stock solution. No chemical analyses of the test concentrations were conducted.
The following mortalities (of a total of 7 fish) were observed in the definitive test at the different test substance concentration after 96 h of exposure: 64 mg/l (7), 32 rng/l (7),16 mg/l (4), 8 mg/l (1) and g mg/l (1). Non mortality was observed in the control after 96 h of exposure.
Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the bank, were not observed.
Based on these data the noninal lethal concentration (LC50) of test item to Poecilla reticulata was calculated to be 14.3 mg/l.
The no-observed effect concentration (NOEC) was 8 mg/l since the one fish that died was considered not to represent a significant effect as defined by the validity criteria of the test.
100 % mortality (LC100) was seen at the nominal concentration of 32 mg/l.
Conclusion
LC50 (96h): 14.3 mg/l (nominal)
Reference
Description of key information
LC50 (96h): 14.3 mg/l (nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 14.3 mg/L
Additional information
The acute lethal toxicity of test item to Poecilla reticulata was investigated under static exposure conditions over a period of 96 hours. The individual test concentrations were prepared by dilution of a stock solution. No chemical analyses of the test concentrations were conducted. The noninal lethal concentration (LC50) was calculated to be 14.3 mg/l; the no-observed effect concentration (NOEC) was 8 mg/l, while the 100 % mortality (LC100) was seen at the nominal concentration of 32 mg/l. Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the bank, were not observed.
An old test performed on the substance under registration is also available and indicate a LC50 value at 48 hours of 2 mg/l. The LC0 was indicated to be 1 mg/l, while the LC100 was idicated at 5 mg/l (reference, 1977). There are no details on testing method and procedures followed; only the sheet of results is available, thus reliability cannot be assigned and the data has not been used for classification.
Data on the structural analogous Similar Substance 02 have also been taken into consideration, in order to clarify the potential toxicity of the substance to fish; the read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
The acute lethal toxicity (LC50) of Similar Substance 02 to rainbow trout was investigated under semi-static exposure conditions, over a period of 96 hours. The nominal concentration for the definitive test was established on the basis of the range-finding study outcomes: the substance was tested at 1.0, 10 and 100 mg/l, under static conditions; 100 % of mortality was recorded at the highested tested concentration. Thus, the limit test was conducted at 10 mg/l. During the definitive test, no deaths occurred either in the test vessels, neither in the control one.
REFERENCE
See attachment
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