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EC number: 226-009-1 | CAS number: 5216-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Carcinogenicity
Administrative data
Description of key information
Carcinogenic
Key value for chemical safety assessment
Carcinogenicity: via oral route
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Study duration:
- chronic
- Species:
- mouse
- System:
- other: many systems involved
- Organ:
- lungs
- lymph node
- mammary gland
- salivary glands
- skin
- stomach
- thymus
Carcinogenicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Carcinogenicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
Under test conditions, the subsatnce shows carcinogenic effects after oral administration at all doses. No information on the mg per kilo of test material administered is available however serious effects involving many organs were seen at all dose levels with a dose dependence.
The toxic effects of this substance are well known: it is in fact listed in Annex VI of the CLP Regulation (EC 1272/2008) with an harmonized classification as Carc. 1B.
Additional information
The authors tested the carcinogenicity of 4-chloro-benzotrichloride (CAS n° 5216-25-1) using a repeated dose methodology exposing female ICR mice to the test substance via oral gavage. The animals were exposed twice a week for 17.5 weeks to 0.05, 0.13, 0.32, 0.8 and 2 µL of the test substance in 1mL of sesame oil. Afterwards the animals were observed for another 14 months. Also control animals were included in the experiment and at the end of the experiment all animals were autopsied and examined for histopathological changes.
Under the test conditions, tumors generally developed earliest in mice exposed to the highest dose. A high incidence of lung tumors and also stomach cancers, skin cancers (squamous cell carcinomas, sarcomas and adenocarcinomas), mammary cancer, salivary gland cancer and malignant lymphoma and thymoma were observed at the end of the experiment especially in the high dose groups. In mice with lung cancer both adenocarcinomas and adenomas were seen in all dose groups with the greatest number (25/31) in the group receiving 0.8 µL of the test substance. Approximately 5 months after first application of the test substance stomach cancers began to develop. The cumulative index of all stomach cancers was less than 25% in each group (exclusion of carcinoma in situ). With the exception of a single differentiated tubular adenocarcinoma in the low dose group, all were epidermoid carcinomas. Furthermore, 11/29 (38%) mice exposed to 2 µL and 3/29 (10%) mice exposed to 0.8µL had malignant lymphoma and thymoma. In the control group only 2 mice out of 26 had tumors whereas the total number of mice with tumors was 6/22, 10/28, 17/22, 27/29 and 25/29 for the doses 0.05, 0.13, 0.32, 0.8 and 2 µL respectively. Hence, the development of tumors happened in a dose responsive manner.
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