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EC number: 270-931-7 | CAS number: 68510-93-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Apr - 12 May 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 04 Apr 1984
- Deviations:
- yes
- Remarks:
- Only four test concentrations instead of the recommended five concentrations.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Directive 84/449/EWG
25 Apr 1984
- Qualifier:
- according to guideline
- Guideline:
- other: "Letale Wirkung beim Zebrabärbling Brachydanio rerio", Empfehlung des Umweltbundesamtest zur Durchführung der Prüfung nach §4 Abs. 1 Nr. 6 der Verordnung über Anmeldeunterlagen und Prüfnachweise nach dem Chemikaliengesetz Texte 16/84, Umweltbundesamt
- Version / remarks:
- 01 May 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-Naphthalenesulfonic acid, 6-diazo-5,6-dihydro-5-oxo-, ester with phenyl(2,3,4-trihydroxyphenyl)methanone
- EC Number:
- 270-931-7
- EC Name:
- 1-Naphthalenesulfonic acid, 6-diazo-5,6-dihydro-5-oxo-, ester with phenyl(2,3,4-trihydroxyphenyl)methanone
- Cas Number:
- 68510-93-0
- Molecular formula:
- all potential esters of C10HH5O3N2SCl and C13H10O4
- IUPAC Name:
- 3-benzoyl-2-hydroxy-6-({[5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl 5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalene-1-sulfonate; 4-benzoyl-2,3-bis({[5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl 5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalene-1-sulfonate; 4-benzoyl-2,3-dihydroxyphenyl 5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalene-1-sulfonate
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10, 22, 50, and 100 mg/L
- Sampling method: At test begin and after 48 and 96 h, 100 mL were sampled from the center of the tank using a glass screw cap bottle.
- Sample storage conditions before analysis: All analyses were performed on the same day samples were taken.
Test solutions
- Vehicle:
- yes
- Remarks:
- Tween 80
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A paste of the test substance was prepared using 0.095 mL Tween 80/L dilution water, which was homogenized with an Ultra-Turrax and ultrasonic bath and added to the test tanks under constant stirring with a glass rod. Then the entire tank content was stirred for ca. 2 h with a KPG-stirrer and glass rod. The test medium presented itself as an orange to red solution or turbid dispersion with substance deposition at the bottom of the tank.
- Controls: Yes, 0 mg/L test substance in test medium (reconstituted water).
- Chemical name of vehicle: Tween 80
- Concentration of vehicle in test medium:0.095 mL Tween 80/L
- Evidence of undissolved material: The test preparation was turbid and substance deposition was observed at the bottom of the test tank.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation: ca. 4 months (Hatching date: 25 Dec 1988; Delivery date: 22 Mar 1989).
- Length at study initiation: mean over all test groups = 2.7 cm (SD = 0.23 cm; Min = 2.3, Max = 3.2 cm)
- Maintenance of the brood fish: The fish were held in dilution water for at least 14 d before test start under the following conditions: Temperature: 22 ± 1 °C; Oxygen: ≥ 80% saturation value; Photoperiod: 12 h; Biomass loading: ≤ 1 g fish/L water; Feeding: twice a day, ad libitum; Food type: Tetra Min, Tetra Werke, Melle, Germany
FEEDING DURING TEST
No feeding during test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- control: 21.8 - 22.9
treatment: 21.8 - 22.9 - pH:
- control: 7.4 - 8.2
treatment: 7.5 - 8.1 - Dissolved oxygen:
- control: 6.8 - 9.5
treatment: 5.3 - 8.6 - Nominal and measured concentrations:
- Control, vehicle control, 10, 22, 50, and 100 mg/L (nominal)
NA, NA, 9.4, 22.3, 50.2, and 95.9 mg/L (mean measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: test tank
- Material, size, headspace, fill volume: Glass test tanks (Length = 30 cm, Width = 22 cm; Height = 22 cm) filled with 10 L test solution.
- Aeration: Before the test the dilution water (reconstituted water) was aerated until oxygen saturation was reached.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: ≤ 1 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water, according to ISO/DIS 7346/1
- Culture medium different from test medium: Same as test.
- Intervals of water quality measurement: The pH value, oxygen concentration and temperature were measured after 2 - 4, 24, 48, 72, and 96 h. Test item concentrations were measured after 0, 48, and 96 h.
OTHER TEST CONDITIONS
- Photoperiod: 12 h light/12 h dark
- Light intensity: 700 Lux
- Other: The test tanks were placed in a water bath made of Hostalit Z with a transparent Plexiglass screen. The water bath was adjusted to 22 ± 1 °C. No aeration of tanks during test.
EFFECT PARAMETERS MEASURED:
- Mortality: after 2-4, 24, 48, 72, and 96 h
- Aspect & behaviour: after 2-4, 24, 48, 72, and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: Yes.
- Results used to determine the conditions for the definitive study: Yes. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 22 - <= 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: Behavioural abnormalities were observed at the lowest concentration and higher and after 2-4 h and later. These included: increased activity, braced gills, irregular breathing, disequilibrium, heightened startle response, cramps, lethargy, trembling, spinning, etc.
- Mortality of control: 0 % (control), 0% (vehicle control)
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The substance deposited at the bottom of the tank. - Reported statistics and error estimates:
- A Probit analysis was not possible with the available data. Therefore, a range was provided as LC50 value.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
ANALYTICAL RESULTS
The measured substance concentrations were > 80% of the nominal concentrations (Table 1). All results correspond to the degradation product. Degradation was complete after 2 h.
Table 1. Measured concentrations in test solutions.
Nominal
Mean measured concentration
[mg/L]
[mg/L]
%
10
9.4
94.0
22
22.3
101.4
50
50.2
100.4
100
95.9*
95.6
* mean of 0 and 48 h
BIOLOGICAL RESULTS
Due to the deep orange colour of the test solutions the observation of symptoms was limited in the 22 – 100 mg/L treatments. At the tested concentrations, altered swimming behavior and impaired breathing was observed. Some fish also showed alterations in behavior and physical aspect. Dead fish paled, had reddened gills and substance deposition on their body surface.
Table 2. Mortality after 96 h.
Concentration
Mortality
[mg/L]
Absolute
Relative [%]
0
0/30
0
0*
0/10
0
10
0/10
0
22
0/10
0
50
8/10
80
100
10/10
100
0* = vehicle control with 0.095 mL Tween 80/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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