Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 272-723-1 | CAS number: 68909-93-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993 - 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, without deviations from standard test guidelines. Analytical measurements on test material not conducted except for TOC
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- In range finding test the correct WAF conc. not specified in protocol, the vortex during WAF formulation did not extend correct distance to bottom of vessel, and daphhid wts could not be verified. Deviations not expected to affect integrity of results.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Phosphorodithioic acid, mixed O,O-bis(2-ethylhexyl and iso-Pr) esters, zinc salts
- EC Number:
- 272-723-1
- EC Name:
- Phosphorodithioic acid, mixed O,O-bis(2-ethylhexyl and iso-Pr) esters, zinc salts
- Cas Number:
- 68909-93-3
- Molecular formula:
- Too complex
- IUPAC Name:
- Phosphorodithioic acid, mixed O,O-bis(2-ethylhexyl and iso-Pr) esters, zinc salts
- Details on test material:
- - Name of test material (as cited in study report): CMA 601
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 1.0, 1.5, 2.5, 5.0, and 6.0 mg/L WAF nominal concentrations
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Each of the five WAFs was prepared by combining the appropriate amount of test substance and dilution water in a glass mixing vessel equipped with a magnetic stirrer (the vortex extended from the surface approximately 30 to 50% of the way to the bottom of the mixing vessel) and stirring these mixtures for approximately 24 hours, settling the mixtures for approximately 1 hour, and siphoning the water phase containing the WAFs.
- Eluate: Not applicable
- Differential loading: Preparation of test solutions with individually prepared loading rates
- Controls: Untreated negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable.
- Evidence of undissolved material (e.g. precipitate, surface film, etc):No insoluble material was noted in test vessels containing test substance
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water Flea
- Strain: Daphnia magna
- Source: In house laboratory cultures with a known history. The original culture was obtained from Aquatic Research Organisms on May 26, 1992.
- Age at study initiation (mean and range, SD): juvenile daphnids less than 24 h old
- Weight at study initiation (mean and range, SD): control daphnids had an average wet weight of 0.52 mg at the end of the test
- Length at study initiation (length definition, mean, range and SD): no data available.
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data available.
- Peripheral shell growth removed prior to test initiation: no data available.
- Method of breeding: Prior to testing daphnid cultures were maintained in 100% dilution water under static renewal conditions and they were provided with yeast/trout chow and the freshwater alga Selenastrum capricornutum each day
- Feeding during test : The daphnids received no food during exposure
- Food type: Not applicable.
- Amount: Not applicable.
- Frequency: Not applicable.
ACCLIMATION
- Acclimation period: from parental daphnids derived from in house laboratory cultures
- Acclimation conditions (same as test or not): Not applicable.
- Type and amount of food: Each culture was fed daily with a suspension of freshwater algae
- Feeding frequency: daily
- Health during acclimation (any mortality observed): The daphnids were free of apparent sickness, injuries and abnormalities at the beginning of the test and there was no mortality in the culture during the 10 days before the start of the test.
QUARANTINE (wild caught)
- Duration: Not applicable.
- Health/mortality: Not applicable.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 0, 3, 24, and 48 hours
Test conditions
- Hardness:
- The dilution water had a hardness of 172 mg/L as CaCO3
- Test temperature:
- The dilution water had a hardness of 172 mg/L as CaCO3
- pH:
- There were no treatment related differences in pH. pH values ranged from 8.5 to 8.6 at the start of the test, 8.4 after 24 hours, and from 8.2 to 8.3 after 48 hours.
- Dissolved oxygen:
- There were no treatment related differences in dissolved oxygen concentration (8.3 to 8.8 at start of test, 8.7 to 9.0 after 24 hours, and 8.7 to 9.0 after 48 hours)
- Salinity:
- not applicable/freshwater
- Nominal and measured concentrations:
- Nominal loading rates of 0, 1, 1.5, 2.5, 4.0, and 6.0 mg/L WAF.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Type (delete if not applicable): Vessels were loosely covered
- Material, size, headspace, fill volume: 250 mL glass vessels containing 200 mL of test solution; water depth was approximately 6 cm.
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable.
- Renewal rate of test solution (frequency/flow rate): static.
- No. of organisms per vessel: 10 per test and control vessel; 20 per concentration
No. of vessels per concentration (replicates): duplicate test vessels
- No. of vessels per control (replicates): 2 vessels in control
- No. of vessels per vehicle control (replicates): Not applicable.
- Biomass loading rate: 0.026 g/L in controls at end of test
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Carbon filtered, dechlorinated tap water collected at TR Wilbury Labs in Marblehead Massachusetts. Water was adjusted to a hardness of 172 mg/L and stored in polyethylene tanks where it was aerated and recirculated through particle filters, activated carbon, and ultraviolet sterilizer. Chemical analysis indicated that the water contained less than 1 ug/L organochlorine pesticides and PCBs.
- Total organic carbon: 1.11 mg C/l
- Particulate matter: 0.1 mg/L
- Metals: NDA
- Pesticides: less than 1 ug/L
- Chlorine: Dechlorinated
- Alkalinity: NDA
- Ca/mg ratio: NDA
- Conductivity: Conductivity in the controls ranged from 630 umhos/cm at the start of the test to 640 after 48 hours.
- Culture medium different from test medium: No difference
- Intervals of water quality measurement: N/A
OTHER TEST CONDITIONS
- Adjustment of pH: NA
- Photoperiod: 16 hours light and 8 hours darkness cycle.
- Light intensity: Cool white fluorescent lights that provided a light intensity of 20 uEin/m2sec.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilisation, sublethal effects, and survival, recorded initially, after 3 hours, 24 hours, and 48 hours.
TEST CONCENTRATIONS
- Range finding study. The loading rates to be used in the definitive study were determined by a preliminary range finding study.
- Test concentrations: In the range finding study daphnids were exposed to the WAF of 1, 10, and 100 mg/L mixtures of CMA601 and water.
- Results used to determine the conditions for the definitive study: At the conclusion of the exposure 100% of the daphnids exposed to 10 and 100 mg/L WAFs were dead or immobilized and 10% of the daphnids exposed to the 1 mg/L WAF were immobilized - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 4 to 6 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: NDA
- Observations on body length and weight: NDA
- Other biological observations: NDA
- Mortality of control: 0
- Other adverse effects control: None noted
- Abnormal responses: NDA
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No insoluble material was noted in test vessels containing CMA 601 throughout the test. - Reported statistics and error estimates:
- Results of the toxicity test were interpreted using standard statistical techniques, including the binomial/nonlinear interpolation method
Any other information on results incl. tables
Table 1. Survival Data from the toxicity test with the WAF of 5 concentrations of Test Material and the daphnid, Daphnia magna
|
Number of Survivors |
|||
Nominal Concentration WAF (mg/L) |
0 hour |
3 hour |
24 hour |
48 hour |
0, Control |
10 10 |
10 10 |
10 10 |
10 10 |
1.0 |
10 10 |
10 9 |
10 9 |
10 9 |
1.5 |
10 10 |
10 10 |
10 10 |
10 9 |
2.5 |
10 10 |
10 10 |
10 10 |
10 10 |
4.0 |
10 10 |
10 10 |
10 10 |
10 10 |
6.0 |
10 10 |
10 10 |
10 10 |
6 5 |
Table 2. Sublethal effect data from the definitive toxicity test with the WAF of 5 concentrations of Test Material and the daphnid, Daphnia magna
|
Number Affected |
|||
Nominal Concentration WAF (mg/L) |
0 hour |
3 hour |
24 hour |
48 hour |
0, Control |
0 0 |
0 0 |
0 0 |
0 0 |
1.0 |
0 0 |
0 0 |
0 0 |
0 0 |
1.5 |
0 0 |
0 0 |
0 0 |
0 0 |
2.5 |
0 0 |
0 0 |
0 0 |
4 1 |
4.0 |
0 0 |
0 0 |
0 0 |
0 0 |
6.0 |
0 0 |
0 0 |
0 0 |
1 5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 value of 5.4 mg/L nominal loading rate with 95% confidence interval from 4 to 6 mg/L. The No Observed Effect Concentration was less than 1 mg/L
- Executive summary:
Introduction. A study was performed to assess the acute toxicity of the test material to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals No 202, as well as the US CFR Title 40, Part 797, Section 1300.
Methods.Following a preliminary range finding study, twenty daphnids (2 replicates of 10 animals) were exposed to Water Accommodated Fractions of the test material over a range of nominal loading rates of 1.0, 1.5, 2.5, 4.0, and 6.0 mg/L. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 0, 3, 24 and 48 hours.
Results.The 48 hour EC50 for the test material to Daphnia magna based on nominal loading rates was 5.4 mg/L with 95% confidence interval from 4 to 6 mg/L. The No Observed Effect Concentration was less than 1 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)