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EC number: 946-533-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- October 29, 2001 - December 7, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - The samples that are taken for analysis: From the first experiment: testvessels with the concentrations: 25; 40 and 63 mg/L
at t=0 and at t=24h
- Sampling method: TOC measurement; of the old and new test solution - Vehicle:
- no
- Details on test solutions:
- A stock solution containing 100,3 mg/l in deionized water was prepared. Its TOC was determined. The TOC was 19,05 mg/L, corresponding to a carbon content of 19%. On each day of the pre-test, a stock solution containing 1000 mg/l in drinking water was prepared. The stock solution then was used to prepare solutions of the concentrations to be tested. On each day of the main study, a stock solution containing 10.000 mg/l in drinking water was prepared. The stock solution then was used to prepare solutions of the concentrations to be tested.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Strain: Hamilton Buchanan
- Source: UBA, Berlin (second generation offspring)
- Age at study initiation (mean and range, SD): approx. 4 months
- Length at study initiation (length definition, mean, range and SD): 2 +/- 1 cm
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: warm water fish food, daphnia, artemisia, tubifex or ground liver
- Feeding frequency: 3 times daily
- Health during acclimation (any mortality observed): < 5% - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- Not reported
- Test temperature:
- 23 +/- 2 degrees Celsius
- pH:
- 7,9 - 8,2 (measured)
- Dissolved oxygen:
- Stayed above 7 mg/l throughout the test
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 0, 16, 19, 22, 25, 40 and 63 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria (10 liter)
- Aeration: yes, 1/s
- Renewal rate of test solution (frequency/flow rate): 1/24 h
- No. of organisms per vessel (pre-test): 3
- No. of organisms per vessel (main test): 7
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 hours - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: expressed as solid content
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 21.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 19 - 25 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: expressed as solid content
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 7.4-9.8
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 9.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: expressed as solid content
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- Not applicable
- Sublethal observations / clinical signs:
Mortalities in the pre-test:
Conc. (mg/L)
Total of fish used
Dead fish; t=24h
Dead fish; t=48h
Dead fish; t=72h
Dead fish; t=96h
actual
cumul.
actual
cumul.
actual
cumul.
actual
cumul.
Blank
3
0
0
0
0
0
0
0
0
1
3
0
0
0
0
0
0
0
0
10
3
0
0
0
0
0
0
0
0
100
3
3
3
0
3
0
3
0
3
Mortalities in the main test:
Conc. (mg/L)
Total of fish used
Dead fish; t=24h
Dead fish; t=48h
Dead fish; t=72h
Dead fish; t=96h
actual
cumul.
actual
cumul.
actual
cumul.
actual
cumul.
Blank (1stexperiment)
7
0
0
0
0
0
0
0
0
Blank (2ndexperiment)
7
0
0
0
0
0
0
0
0
16 (1stexperiment)
7
0
0
0
0
1
1
0
1
19 (2ndexperiment)
7
0
0
0
0
0
0
0
0
22 (2ndexperiment)
7
0
0
0
0
4
4
2
6
25 (1stexperiment)
7
7
7
-
7
-
7
-
7
40 (1stexperiment)
7
7
7
-
7
-
7
-
7
63 (1stexperiment)
7
7
7
-
7
-
7
-
7
Determined TOC-concentrations:
Stock solution (100,3 mg/l): 19,05 mg/L
Nominal conc. Mg/L
TOC-concentration in mg/L (old/new test solution)
0h
24h
48h
72h
96h
new
old
new
old
new
old
new
old
Blank
0
0.02
-
-
-
-
-
-
25
2.49
8.03
-
-
-
-
-
-
40
4.40
4.94
-
-
-
-
-
-
63
9.64
3.17
-
-
-
-
-
-
100*
15.40
20.29
-
-
-
-
-
-
*This solution was aerated for 24 hours and then the TOC was measured.
- Validity criteria fulfilled:
- yes
- Remarks:
- (no mortalities occurred in the controls; concentration of dissolved oxygen stayed above 7 mg/L or 81% throughout the test)
- Conclusions:
- 96h-LC50: 21.8 mg/L (based on test material)
96h-LC50: 8.5 mg/L (based on solid content) - Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- December 12, 1994 - January 13, 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- no analytical monitoring
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Cited as Directive 92/69/EEC, C.1
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- See section Any other information on materials and methods.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- See section Any other information on materials and methods
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- 180 to 208 ppm as CaCO3
- Test temperature:
- 14.7 to 15.4°C (definitive test)
- pH:
- 7.8 to 8.5
- Dissolved oxygen:
- 61 to 95% of saturation
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- See section Any other information on materials and methods
- Details on test conditions:
- See section Any other information on materials and methods
- Reference substance (positive control):
- yes
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: expressed as solid content
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: expressed as solid content
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 3 - 6 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 15.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: expressed as solid content
- Basis for effect:
- mortality (fish)
- Details on results:
- Validity criteria fulfilled: yes (0% mortality in the control).
- Results with reference substance (positive control):
- Lindane: CL50-24 h: 0.055 ppm (w/w) (nominal concentration) - 95% confidence interval: 0.027-0.068 mg/l (calculated with the binomial method). The data are in conformity with the historical data of the laboratory.
- Reported statistics and error estimates:
- Not applicable
- Sublethal observations / clinical signs:
Toxicity to fish (definitive test):
Concentrations ppm (w/w) expressed as solid content
N° vesselNumber of dead fish
3h
24h
48h
72h
96h
Control1
0
0
0
0
0
2
0
0
0
0
0
103
0
0
5
-
-
4
0
0
5
-
-
175
0
4
1
-
-
6
0
3
3
-
-
327
4
1
-
-
-
8
4
1
-
-
-
609
5
-
-
-
-
10
5
-
-
-
-
10011
5
-
-
-
-
12
5
-
-
-
-
Toxicity to fish (complementary test):
Concentrations ppm (w/w) expressed as solid content
N° vesselNumber of dead fish
3h
24h
48h
72h
96h
Control1
0
0
0
0
0
2
0
0
0
0
0
23
0
0
0
0
0
4
0
0
0
0
0
35
0
0
0
0
0
6
0
0
0
0
0
67
0
1
2
2
-
8
0
2
2
1
-
109
5
5
-
-
-
10
5
5
-
-
-
1711
5
5
-
-
-
12
5
5
-
-
-
Toxicity to fish (definitive + complementary test):
Concentrations ppm (w/w) expressed as solid content
Test
N° vesselMortality (cumulated) %
24h
48h
72h
96h
ControlD
1 + 2
0
0
0
0
C
1 + 2
0
0
0
0
D+C
-
0
0
0
0
2
C
3 + 4
0
0
0
0
3
C
5 + 6
0
0
0
0
6
C
7 + 8
30
70
100
-
10D
3 + 4
0
100
-
-
C
9 + 10
100
-
-
-
D + C
-
50
100
-
-
17D
5 + 6
60
100
-
-
C
11 +12
100
-
-
-
D + C
-
80
100
-
-
32
D
7 + 8
100
-
-
-
60
D
9 + 10
100
-
-
-
100
D
11 + 12
100
-
-
-
D: definitive test
C: complementary test
D + C: mean of the mortality definitive test + complementary test
Physical-chemical measurements (definitive test):Concentrations
ppm (w/w) expressed as solid content
N° vesselpH
Dissolved oxygene (%)
24 h
48h
72h
96h
24 h
48h
72h
96h
Control1
7.8
7.9
7.9
7.9
68
69
68
70
2
7.8
7.9
7.9
7.9
63
67
68
71
103
7.8
8.1
-
-
68
87
-
-
4
7.8
8.2
-
-
68
86
-
-
175
8.0
8.3
-
-
73
93
-
-
6
8.0
8.3
-
-
68
93
-
-
327
8.3
-
-
-
90
-
-
-
8
8.4
-
-
-
90
-
-
-
609
8.4
-
-
-
89
-
-
-
10
8.4
-
-
-
91
-
-
-
10011
8.4
-
-
-
95
-
-
-
12
8.4
-
-
-
94
-
-
-
Physical-chemical measurements (complementary test):
Concentrations
ppm (w/w) expressed as solid content
N° vesselpH
Dissolved oxygene (%)
24 h
48h
72h
96h
24 h
48h
72h
96h
Control1
7.9
8.0
8.0
7.9
65
73
71
69
2
7.9
8.0
7.9
7.9
61
67
63
65
23
8.0
8.0
7.9
7.9
62
67
69
65
4
8.0
8.1
7.9
8.0
61
68
70
72
35
7.9
8.0
7.9
7.9
65
69
69
68
6
7.9
8.0
7.9
7.9
65
65
66
65
67
8.0
8.2
8.3
-
65
78
94
-
8
8.0
8.3
8.3
-
66
86
94
-
109
8.3
-
-
73
-
-
-
10
8.4
-
-
78
-
-
-
1211
8.5
-
82
-
-
-
12
8.5
-
-
92
-
-
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96h-LC50: 10.6 mg/L (based on test material)
96h-LC50: 4.2 mg/L (based on solid content) - Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2007-06-05 to 2007-06-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: highest concentration and control
- Sampling Method: 2 mL from the approximate centre of the test vessel at t=0 h, t = 24 h and t = 96 h
- Sample storage conditions before analysis: Samples were stored in a freezer until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of test solutions started with two solutions of 100 mg a.i./L (198 mg/L, based on formulation) applying 16 minutes of magnetic stirring to accelerate the dissolving of the test substance in the test medium, after which the solutions were pooled. Part of the solution was used as highest test concentration and part was used to prepare the lower test concentrations.
- Controls: Test medium without test substance or other additives
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The final test solutions were all clear and colourless. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Length at study initiation (length definition, mean, range and SD): 2.7 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.56 ± 0.1 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water.
- Feeding during test: No feeding from 24 hours prior to the test and during the total test period.
ACCLIMATION
- Acclimation period: At least 12 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: pelleted fish food (Nutra 3.0 T, TROUW NUTRITION, Putten, The Netherlands)
- Feeding frequency: Daily
- Health during acclimation (any mortality observed):In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3
- Test temperature:
- control: 20.7 - 21.4°C
100 mg/L: 20.9 - 21.1°C - pH:
- control: 7.4 - 7.6
100 mg/L: 7.2 - 7.5 - Dissolved oxygen:
- control: 5.9 (aeration introduced on day 2) - 8.9 mg/L
100 mg/L: 6.0 (aeration introduced on day 2) - 8.9 mg/L - Nominal and measured concentrations:
- nominal: 0, 0.1, 1.0, 10 and 100 mg/L
Analysis of the samples taken during the combined limit/range-finding test showed that measured concentrations were stable and in agreement with nominal throughout the 96-hour test period (96-98%) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 10 L, all-glass, containing 9 L of test solution.
- Aeration: The test media were not aerated, except for a period of 22.5 hours during nominal days 2 and 3 of the test.
- No. of organisms per vessel: 3 (0.1, 1, 10 mg a.i./L), 7 (control and 100 mg a.i./L)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: Control and 100 mg a.i./L: 0.44 g fish/L; 0.10, 1.0 and 10 mg a.i./L: 0.19 mg fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, nitrate and nitrite concentration and ammonia concentration: once a week. Temperature: daily.
In addition, pH and temperature were measured before transferring the fish to the test system.
OTHER TEST CONDITIONS
- Adjustment of pH: yes, pH 7.7 ± 0.3
- Photoperiod: 16 h/d
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortality and sublethal effects at 4, 24, 48, 72 and 96 hours. In addition, every afternoon from day 0 and every morning from day 1 to observe for any dead or severely distressed fish.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Test concentrations: 0, 0.1, 1, 10 and 100 mg a.i./L - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality or clinical effects were observed at any of the concentrations tested.
- Results with reference substance (positive control):
- - Results with reference substance valid: Yes
96h LC50 = 0.15 mg/L (95% ci 0.10 - 0.22 mg/L)
range of historical data: 96h LC50 0.10 - 0.46 mg/L - Sublethal observations / clinical signs:
Analysis of the samples taken during the combined limit/range-finding test showed that measured concentrations were stable and in agreement with nominal throughout the 96-hour test period (96-98%).
All test conditions remained within the ranges prescribed by the protocol (pH: 6.0-8.5, constant within 1 unit; temperature 20-24°C, constant within 2°C; oxygen > 60% of air saturation). Aeration was introduced after 48 hours of exposure, as the oxygen concentration tended to drop below the optimum level for testing with carp, i.e. below 5 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present test Amphopropionate C8 induced no visible effects in carp at or below 100 mg a.i./L. Hence, the 96 h LC50, exceeded 100 mg/L, based on active ingredient concentrations.
- Executive summary:
In a 96 h acute toxicity study according to OECD 203 guideline (1992) and EU Method C.1 (1992), carp (Cyprinus carpio) were exposed to Amphopropionate C8 (50.6% a.i.) at nominal concentrations of 0 (control), 0.1, 1.0, 10 and 100 mg a.i./L under static conditions. Analysis of the samples taken during the combined limit/range-finding test showed that measured concentrations were stable and in agreement with nominal throughout the 96-hour test period (96-98%). 3 test organisms were exposed to 0.1, 1 and 10 mg a.i./L and 7 test organisms to 0 mg a.i./L (control) and 100 mg a.i./L.
The 96 h LC50 was > 100 mg a.i./L. The EC50 and NOEC values, based on mortality/sublethal effects, were both > 100 mg a.i./L. No visible sublethal effects were observed under the conditions of the present test.
This toxicity study is classified as acceptable and satisfies the guideline requirement for an OECD 203 guideline and EU Method C.1 toxicity study.
Results Synopsis
Test organism size/age: Cyprinus carpio (mean length: 2.7 ± 0.1 cm; mean weight: 0.56 ± 0.1 g)
Test Type: static
LC50: > 100 mg a.i./L
NOEC: > 100 mg a.i./L
Endpoint(s) Effected: mortality and sublethal effects
Referenceopen allclose all
Description of key information
96 h LC50 = 4.2 mg/L (Oncorhynchus mykiss; OECD TG 203; RL2; GLP) read-across: Amphoacetates C8-C18
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4.2 mg/L
Additional information
No experimental data on acute toxicity to fish are available for the target substance Amphopropionates C12-18. However, short-term toxicity studies are available for the closely related source substances Amphopropionate C8 and Amphoacetates C8-C18. A justification for read-across is given in the Endpoint summary Aquatic toxicity.
In a 96 h acute toxicity study according to OECD Guideline 203 (1992) and EU Method C.1 (1992), carp (Cyprinus carpio) were exposed to Amphopropionate C8 (50.6% a.i.) at nominal concentrations of 0 (control), 0.1, 1.0, 10 and 100 mg a.i./L under static conditions. Analysis of the samples taken during the combined limit/range-finding test showed that measured concentrations were stable and in agreement with nominal throughout the 96-hour test period (96-98%). 3 test organisms were exposed to 0.1, 1 and 10 mg a.i./L and 7 test organisms to 0 mg a.i./L (control) and 100 mg a.i./L.
The 96 h LC50 was > 100 mg a.i./L. The EC50 and NOEC values, based on mortality/sublethal effects, were both > 100 mg a.i./L. No visible sublethal effects were observed under the conditions of the present test.
In a 96 h acute toxicity study according to OECD Guideline 203 and EU Method C.1, rainbow trout (Oncorhynchus mykiss) were exposed to Amphoacetates C8-C18 at nominal concentrations of 0 (control), 10, 17, 32, 60 and 100 mg/L in the definitive test and 0 (control), 2, 3, 6, 10 and 17 mg/L in the complementary test under semi-static conditions. Analytical monitoring was not performed.
The 96 h LC50 based on test material was 10.6 mg/L. The 96 h LC50 based on solids content was 4.2 mg/L.
In a 96 h acute toxicity study according to OECD Guideline 203 and EU Method C.1, zebra fish (Danio rerio) were exposed to Amphoacetates C8-C18 at nominal concentrations of 0 (control), 16, 19, 22, 25, 40 and 63 mg/L under semi-static conditions.
The 96 h LC50 based on test material was 25 mg/L. The 96 h LC50 based on solids content was 8.5 mg/L.
The 96 h LC50 of acrylic acid to Oncorhynchus mykiss was reported to be 27 mg/L.
For chemical safety assessment the lowest 96 h LC50 of 4.2 mg/L will be used.
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