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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure according to national standards

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German industrial standard test guideline DIN 38 412, part L15.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-cyano-N-methylacetamide
EC Number:
228-705-0
EC Name:
2-cyano-N-methylacetamide
Cas Number:
6330-25-2
Molecular formula:
C4H6N2O
IUPAC Name:
2-cyano-N-methylacetamide
Details on test material:
- Name of test material (as cited in study report): N-Methylacetamid in Loesung
- Analytical purity 13% aqueous solution:

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 8.2 cm (range: 7.2-9.4)
- Weight at study initiation: 5.8 g (range: 4.2-8.9)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day
- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Total hardness: 2.5 mmol/L
Alkalinity:
Test temperature:
20 ±1 °C
pH:
7.2-7.5
Dissolved oxygen:
7.3 - 8.2 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 5.8 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Reference substance (positive control):
yes
Remarks:
Chloracetamid

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 300 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: Caluclated from concentration of aqueous solution (13%)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes