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EC number: 241-516-8 | CAS number: 17517-01-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 11, 2010 to March 2, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N-dimethyl-3-(octadecyloxy)propylamine
- EC Number:
- 241-516-8
- EC Name:
- N,N-dimethyl-3-(octadecyloxy)propylamine
- Cas Number:
- 17517-01-0
- Molecular formula:
- C23H49NO
- IUPAC Name:
- dimethyl[3-(octadecyloxy)propyl]amine
- Reference substance name:
- Unknown impurities
- IUPAC Name:
- Unknown impurities
- Test material form:
- liquid
Constituent 1
impurity 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For the determination of the actual test item concentrations, duplicates samples were taken at the start and end of each test medium renewal period. For the 48-hour stability samples, the contents of the respective replicates were combined prior to sampling.
Immediately after sampling, methanol (1 mL methanol per 1-mL sample volume) was added to each sample to stabilize the latter during the storage period. Thereafter, all samples were deep-frozen (at about -20° C).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Reconstituted test water according to ISO 6341was used in the study. It consisted of analytical grade salts dissolved in purified water.
The ratio of Ca:Mg and Na:K was 4:1 and 10:1, respectively, based on molarity.
The test water was aerated prior to the start of the study until oxygen saturation was reached. During the test period, the test water was not aerated.
The following concentrations were tested: dilutions 1:10000, 1:3200, 1:1000, 1:320, 1:100 and 1:32 of a supersaturated stock dispersion with a loading rate of 100 mg/L. Additionally, a control (test Water without test item) was tested in parallel.
Due to the low water solubility of the test item, a dispersion with the loading rate of 100 mg/L was prepared by dispersing 100 mg of the test item (effective weights:100.5 and 100.4 mg for the first and second test medium renewal periods, respectively) in 1000 mL of test water. The dispersion was supported by ultrasonic treatment for 15 minutes and intense stirring by a magnetic stirrer over 96 hours at room temperature in the dark.
The long stirring period of 96 hours was selected according to the results of a pre-experiment (non-GLP) which showed that the maximum concentration of test item was reached after the stirring period of 96 hours.
After the 96-hour stirring period, the supersaturated dispersion of the test item was filtered at first through a Whatman GF/C filter (pore size 1.2 μm), and then through a Whatman GF/F filter (pore size 0.7 μm).Thus, a two-step filtration method was used to separate dissolved and dissolved test item. The negative pressure of the filtration unit was reduced as far as possible. The undiluted filtrate was serially diluted with test water to preparer the test media of all test concentrations. The test media were prepared just before introduction of the daphnids (i.e., start of the test and before the medium renewal) and were filled into equilibrated test vessels.
An equilibration of the test vessels was performed to secure a correct dosage, since in the range-finding tests and pre-experiment to the dosage of the test item, a considerable reduction of the actual test item concentrations in the test media was observed (due to a high adsorption of the test item to the test beakers). Thus, all the beakers prepared were filled with the test medium of the highest test concentration (dilution 1:32) for about 3 hours. After the 3-hour equilibration (immediately before addition of daphnids), the equilibrating medium in each test beaker was replaced with an appropriate freshly prepared test medium.
The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The study was performed with young daphnids of the species Daphnia Magna Straus. A clone of this species (defined by the supplier as clone 5) was originally supplied by the University of Sheffield/ UK in 1992. Since that time, the clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests.
During breeding, daphnids are generally fed three times a week with an algal suspension of the green algae Scenedesmus subspicatus CHODAT, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures and cultivated at Harlan Laboratories under standardized conditions or a mixture of this algal suspension and a commercial fish diet.
At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20ºC
- pH:
- 7.8-8.0
- Dissolved oxygen:
- 8.4 mg/L
- Nominal and measured concentrations:
- Dilution: 1:10000, 1:3200, 1:1000. 1:320, 1:100, 1:32.
Mean measured concentration (µg/L): (n.a.), 2.2, 7.0, 25, 95, 509. - Details on test conditions:
- For each treatment, 20 daphnids were used divided into four replicates of five daphnids each. The volume of test solution provided for each daphnia was 10 mL. Thus, the requirement of the test guidelines for the minimum volume of 2 mL test medium per daphnia was fulfilled. The daphnids were randomly distributed to the test vessels at initiation of the test.
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.8 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 48 hours of exposure, no immobilized test organisms were determined in the control and up to and including the mean measured concentration of 2.2 µg/L. At the next two higher mean measured concentrations of 7.0 and 25 µg/L, the immobilization rate increased to 45 and 95% respectively.
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the entire test duration.
The test is considered to be valid, as in the control, not more than 10% of the daphnids should show immobilization or other signs of disease or stress (e.g., discoloration or unusual behavior such as trapping at the surface water). Furthermore, the dissolved concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels. - Results with reference substance (positive control):
- For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in September 2009 (48-hour EC50: 1.1 mg/L, study C63680) indicated that the sensitivity of the test organisms was within the internal historical range (48-hour EC50 from 1996 to 2009: 0.53-1.1 mg/L).
Any other information on results incl. tables
Effect of the test substance on the Mobility of Daphnia magna
Dilution (loading rate: 100 mg/L) | Mean measured concentration (µg/L) | No. of Daphnids tested | Immobilized daphnids after 24 hours No. |
Immobilized daphnids after 24 hours % |
Immobilized daphnids after 48 hours No. |
Immobilized daphnids after 48 hours % |
Control | - | 20 | 0 | 0 | 0 | 0 |
1: 10000 | n.a | 20 | 0 | 0 | 0 | 0 |
1:3200 | 2.2 | 20 | 0 | 0 | 0 | 0 |
1:1000 | 7.0 | 20 | 8 | 40 | 9 | 45 |
1:320 | 25 | 20 | 9 | 45 | 19 | 95 |
1:100 | 95 | 20 | 20 | 100 | 20 | 100 |
1:32 | 509 | 20 | 20 | 100 | 20 | 100 |
pH Values in the Treatments
Dilution (loading rate: 100 mg/L) | Exposure time 0h NEW |
Exposure time 24 h OLD |
Exposure time 24 h NEW |
Exposure time 48 h OLD |
Control | 7.8 | 7.9 | 7.9 | 7.9 |
1:10000 | 7.8 | 7.9 | 7.9 | 7.9 |
1:3200 | 7.8 | 7.9 | 7.9 | 7.9 |
1:1000 | 7.8 | 7.9 | 7.9 | 8.0 |
1:320 | 7.8 | 8.0 | 7.9 | 8.0 |
1:100 | 7.9 | 8.0 | 7.9 | 8.0 |
1:32 | 7.9 | 8.0 | 7.9 | 8.0 |
Dissolved Oxygen Concentrations (mg/L) in the Treatments
Dilution loading rate: 100 mg/L | Exposure time 0h NEW |
Exposure time 24h OLD |
Exposure time 24h NEW |
Exposure time 48h OLD |
Control | 8.5 | 8.6 | 8.4 | 8.4 |
1:10000 | 8.6 | 8.5 | 8.4 | 8.4 |
1:3200 | 8.7 | 8.4 | 8.6 | 8.6 |
1:1000 | 8.7 | 8.5 | 8.6 | 8.6 |
1:320 | 8.5 | 8.6 | 8.5 | 8.7 |
1:100 | 8.6 | 8.6 | 8.4 | 8.5 |
1:32 | 8.6 | 8.7 | 8.4 | 8.5 |
Temperature (ºC) in the Treatments
Dilution loading rate: 100 mg/L | Exposure time 0h NEW |
Exposure time 24h OLD
|
Exposure time 24h NEW
|
Exposure time 48h OLD |
Control |
20 |
20 |
20 |
20 |
1:10000 |
20 |
20 |
20 |
20 |
1:3200 |
20 |
20 |
20 |
20 |
1:1000 |
20 |
20 |
20 |
20 |
1:320 |
20 |
20 |
20 |
20 |
1:100 |
20 |
20 |
20 |
20 |
1:32 |
20 |
20 |
20 |
20 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of test item was calculated to be 9.8 μg/L (0.0098 mg/L) with 95% confidence limit of 6.1 and 16 μg/L (0.0061 and 0.016 mg/L).
- Executive summary:
The acute toxicity of the test item N,N-Dimethyl-3-octadccyloxypropylamine to Daphnia magna was determined in a 48-hour semi-static test according to the EU Commission Directive 92/69/EEC, Part C.2, the Commission Regulation (EC) No 440/2008, Part C.2 and the OECD Guideline for Testing of Chemicals,No.202 (2004).
Due to the low water solubility of the test item, a dispersion of the test item with the loading rate of 100 mg/L was continuously stirred at room temperature in the dark over 96 hours before each test media preparation. Then, the dispersion was filtered using a two step filtration. The dilutions l:10000, 1:3200, 1:1000, 1:320, 1:100 and l:32 of the supersaturated dispersion were used as test media (after a preceding 3-hour equilibration of all test beakers with the test medium of the highest test concentration (dilution l:32) for correct dosage). A control was tested in parallel.
The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixture, 2000.
At the start of the renewal periods (day 0/day l), the measured concentrations of the test item in the analyzed test media (dilutions l:3200, 1:1000, 1:320, 1:100 and l:32) amounted to 5.0/2.0, 17/7.0 48/22, 156/65 and 633/393 μg/L. At the end of the renewal periods (day 1/day 2), the measured concentrations were l.4/1.5, 4.3/4.5, 22/14, 104/59 and 603/409 μg/L, respectively.
The biological results were based on the mean measured concentrations of the test item in the test media calculated as geometric means of the concentrations measured at the start and end of the renewal periods.
The 48-hour EC50 of test item was calculated to be 9.8 μg/L (0.0098 mg/L) with 95% confidence limit of 6.1 and 16 μg/L (0.0061 and 0.016 mg/L).
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