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EC number: 246-874-9 | CAS number: 25340-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This is a GLP study.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Diethylbenzene
- EC Number:
- 246-874-9
- EC Name:
- Diethylbenzene
- Cas Number:
- 25340-17-4
- Molecular formula:
- C10H14
- IUPAC Name:
- diethylbenzene
- Details on test material:
- IUCLID4 Test substance: as prescribed by 1.1 - 1.4
Mixed Diethylbenzenes (CAS No. 25340-17-4)
Purity:Not specified
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Details on exposure:
- Type: Micronuclei formation in bone marrow erythrocytes
Vehicle and positive controls were dosed intraperitoneally with peanut oil and cyclophosphamide, respectively.
Number of animals: 18/sex/group, except for the positive control group (5/sex). Bone marrow smears, 5 animals/sex/dose, were made at approximately 24, 48, and 72 hours post-dosing, according to the method of Schmid (1976). The slides were fixed in methanol and stained with 5% Giemsa for approximately 20 minutes. The percentage of PCE was calculated by counting a total of >200 erythrocytes. On each slide, a total of 1000 PCEs were evaluated for the presence of micronuclei.
Schmid, W (1976). The micronucleus test for cytogenetic analysis. In: A. Hollaender (Ed), Chemical Mutagens. - Duration of treatment / exposure:
- Single dose
Doses / concentrationsopen allclose all
- Dose / conc.:
- 1 000 other: mg/kg
- Dose / conc.:
- 2 000 other: mg/kg
- Dose / conc.:
- 4 000 other: mg/kg
- No. of animals per sex per dose:
- 18/sex/group, except for the positive control group (5/sex).
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
Examinations
- Tissues and cell types examined:
- Bone marrow smears
- Details of tissue and slide preparation:
- Bone marrow smears, 5 animals/sex/dose, were made at approximately 24, 48, and 72 hours post-dosing, according to the method of Schmid (1976). The slides were fixed in methanol and stained with 5% Giemsa for approximately 20 minutes.
- Evaluation criteria:
- The percentage of PCE was calculated by counting a total of >200 erythrocytes. On each slide, a total of 1000 PCEs were evaluated for the presence of micronuclei.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- Cytotoxicity was noted in females dosed with 4000 mg/kg and sampled at 48 hours.
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Cytotoxicity: Cytotoxicity was noted in females dosed with 4000 mg/kg and sampled at 48 hours.
Genotoxic effects: No treatment-related effect on micronucleated polychromatic erythroctyes in either sex.
Toxicity NOEL: 1000 mg/kg
Genotoxicity NOEL > 4000 mg/kg
Statistical results: No statistically significant differences between mixed diethylbenzene-exposed animals and controls.
Remarks: Evidence of toxicity was found at > 2000 mg/kg in both sexes. Clinical signs of toxicity observed were decreased motor activity, collapse, labored breathing, convulsions, and weakness. In addition, on Day 1, one male at 2000 mg/kg and three males and one female at 4000 mg/kg died before the scheduled sampling time; two moribund females at 4000 mg/kg were euthanized.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Under the conditions of this study, mixed diethylbenzenes was considered non-genotoxic, since micronuclei were not induced in the bone marrow erythrocytes of mice, even at doses that exceeded the MTD.
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