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EC number: 226-615-6 | CAS number: 5437-98-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989 - 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4'-methoxyacetoacetanilide
- EC Number:
- 226-615-6
- EC Name:
- 4'-methoxyacetoacetanilide
- Cas Number:
- 5437-98-9
- Molecular formula:
- C11H13NO3
- IUPAC Name:
- N-[4-(methoxyacetyl)phenyl]acetamide
- Details on test material:
- Purity: > 99 %
Constituent 1
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: 1. test 2,8 +- 0.18 cm; 2. test 3,0 +-0,39 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice a day ad libitum
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21,0 - 22,2 °C
- pH:
- 7,6 - 8,2
- Dissolved oxygen:
- 6,3 - 8,7 mg O2/L
- Nominal and measured concentrations:
- nominal (mg/L) measured (mg/L)
220 211
500 491 - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass container (30 cm x 22 cm x 24 cm) calibrated to 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
OTHER TEST CONDITIONS
- Photoperiod: 12 hours illumination from upside
- Light intensity: about 700 Lux - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LD50 is 331,7 mg/L.
- Executive summary:
The test item was tested for acute toxicity to Brachydanio rerio according to OECD guideline 203 for 96 hours. Concentrations of 220 mg/L and 500 mg/L were applied . For each concentration 10 fish were used. The 220 mg/L-group showed only symptoms but no letality. The 500 mg/L-group showed symptoms and letality. Out of the results a LD50 value of 331,7 mg/L could be determined.
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